A new artificial diet for the diamondback moth,Plutella Xylostella(L), had been selected out successfully. The diet contained the following constituents: soybean powder, wheat germ, wheat bran powder, brewer's ye...A new artificial diet for the diamondback moth,Plutella Xylostella(L), had been selected out successfully. The diet contained the following constituents: soybean powder, wheat germ, wheat bran powder, brewer's yeast and other constituents. So far, the diamondback; moth had been reared on this artificial diet for 25 generations and still mantained its normal biological characteristics. Under 25±1℃ and 60%￣75%RH with 12 h PH,the results of rearing the diamondback moth on the diet as follows:egg hatch,81. 3%￣94. 7 %; survival from eggsto pupae, 55. 0% ￣ 76. 7 % 1 pupal survival, 79. 7 % ￣ 100 % ; adult emergence, 80% ￣ 100 %; fecundity 94. 7￣ 144.4 eggs/ ;pupal weight, 90. 4￣110. 8 mg/20 pupae; average days to adult: , 10. 2; 13. 1. The diet is not only simpler on the constituents but also have better r-earing results and more rearing generations.展开更多
The cDNA containing full encoding region or E1 antigen or HCV was cloned into an expression plasmid pRSETHisB. The recombinant plasmid PRSETE1 was introduced into the BL21 (DE3) strain or E. coli. The engineering bact...The cDNA containing full encoding region or E1 antigen or HCV was cloned into an expression plasmid pRSETHisB. The recombinant plasmid PRSETE1 was introduced into the BL21 (DE3) strain or E. coli. The engineering bacteria harhouring the pRSETEI was cultivated in 2YT medium at 37℃.When the Expression of E1 protein was induced by 1 mmol IPTG, the bacteria was killed and the number of living cell was droned down from 107 to 103 cell/mL one hour post induction. suggest that E1 protein is poisoned to E. coli. However, the 26kD polypeptide or E1 fussion still synthesized in appropriate condition. The expression level was about 10% or total protein 4 h after Inducing. Th. E1 protin was purfied by Ni2+-NTAAgarose column chromatography to homogeneous. The purified E1 protein was sensitive and specific in reaction with anti-HCV antibody in sera.展开更多
Human Cytomegalovirus (HCMV) DNA polymerase gene was overexpressed in insect cells using the baculovirus transfer system. A6. 2 kb HCMV Rsr Ⅱ-EcoRI DNA fragment with intact HCMV pci gene coding sequence was engineere...Human Cytomegalovirus (HCMV) DNA polymerase gene was overexpressed in insect cells using the baculovirus transfer system. A6. 2 kb HCMV Rsr Ⅱ-EcoRI DNA fragment with intact HCMV pci gene coding sequence was engineered into NheI site of vector pBlueBac under the control of polyhedrin promoter of Autographa californica nuclear polyhedrosis virus (AcNPV). Recombinant AcNPV carried HCMV pci gene was generated by cotransfection of Spodoptera frugiperta cell (SF21) with AcNPV DNA and baculovirus transfer vector with HCMV pci gene. In fection of SF21 cell with recombinant virus lead to the expression of 140 kD peptide of HCMV specific DNA polymerase at the level approximately 2 mg per 108 cells. The polypeptide was purified from the infected SF21 cells by a series of column chromatography to homogeneity. The purified enzyme had a molecular weight of 140 kD and reacted with antiserum specific for HCMV DNA polymerase. It exhibited both 3'-5'and 5'-3' exonuclease activities.This enzyme is also sensitive to phosphono acetate.展开更多
文摘A new artificial diet for the diamondback moth,Plutella Xylostella(L), had been selected out successfully. The diet contained the following constituents: soybean powder, wheat germ, wheat bran powder, brewer's yeast and other constituents. So far, the diamondback; moth had been reared on this artificial diet for 25 generations and still mantained its normal biological characteristics. Under 25±1℃ and 60%￣75%RH with 12 h PH,the results of rearing the diamondback moth on the diet as follows:egg hatch,81. 3%￣94. 7 %; survival from eggsto pupae, 55. 0% ￣ 76. 7 % 1 pupal survival, 79. 7 % ￣ 100 % ; adult emergence, 80% ￣ 100 %; fecundity 94. 7￣ 144.4 eggs/ ;pupal weight, 90. 4￣110. 8 mg/20 pupae; average days to adult: , 10. 2; 13. 1. The diet is not only simpler on the constituents but also have better r-earing results and more rearing generations.
文摘The cDNA containing full encoding region or E1 antigen or HCV was cloned into an expression plasmid pRSETHisB. The recombinant plasmid PRSETE1 was introduced into the BL21 (DE3) strain or E. coli. The engineering bacteria harhouring the pRSETEI was cultivated in 2YT medium at 37℃.When the Expression of E1 protein was induced by 1 mmol IPTG, the bacteria was killed and the number of living cell was droned down from 107 to 103 cell/mL one hour post induction. suggest that E1 protein is poisoned to E. coli. However, the 26kD polypeptide or E1 fussion still synthesized in appropriate condition. The expression level was about 10% or total protein 4 h after Inducing. Th. E1 protin was purfied by Ni2+-NTAAgarose column chromatography to homogeneous. The purified E1 protein was sensitive and specific in reaction with anti-HCV antibody in sera.
文摘Human Cytomegalovirus (HCMV) DNA polymerase gene was overexpressed in insect cells using the baculovirus transfer system. A6. 2 kb HCMV Rsr Ⅱ-EcoRI DNA fragment with intact HCMV pci gene coding sequence was engineered into NheI site of vector pBlueBac under the control of polyhedrin promoter of Autographa californica nuclear polyhedrosis virus (AcNPV). Recombinant AcNPV carried HCMV pci gene was generated by cotransfection of Spodoptera frugiperta cell (SF21) with AcNPV DNA and baculovirus transfer vector with HCMV pci gene. In fection of SF21 cell with recombinant virus lead to the expression of 140 kD peptide of HCMV specific DNA polymerase at the level approximately 2 mg per 108 cells. The polypeptide was purified from the infected SF21 cells by a series of column chromatography to homogeneity. The purified enzyme had a molecular weight of 140 kD and reacted with antiserum specific for HCMV DNA polymerase. It exhibited both 3'-5'and 5'-3' exonuclease activities.This enzyme is also sensitive to phosphono acetate.
