Cytotoxicity and genotoxicity assessment of Euphorbia hirta in MCF-7cell line model using comet assay

Objective:To evaluate the cytotoxicity and genotoxicity activity of Euphorbia hirta(E.hirta)in MCF-7 cell line model using comet assay.Methods:The cytotoxicity of E.hirta extract was investigated by employing brine shrimp lethality assay and the genotoxicity of E.hirta was assessed by using Comet assay.Results:Both toxicity tests exhibited significant toxicity result.In the comet assay,the E.hirta extract exhibited genotoxicity effects against MCF-7 DNA in a time-dependent manner by increasing mean percentage of DNA damage.The extract of E.hirta showed significant toxicity against brine shrimp with an LC_(50)value of 620.382μg/mL(24 h).Comparison with positive control potassium dichroniate signifies that cytotoxicity exhibited by the methanol extract might have moderate activity.Conclusion:The present work confirmed the cytotoxicity and genotoxicity of E.hirta.However,the observed toxicity of E.hta extracts needs to be confirmed in additional studies.

Antioxidant activity of methanol extracts of different parts of Lantana camara

Objective:To investigate the antioxidant activity of methanolic extracts of Lantana camara(L.camara)various parts and the determination of their total phenolics content.Methods:The extract was screened for possible antioxidant activities by free radicaI scavenging activity(DPPH),xanthine oxidase inhibition activity and Griess—llosvay method。Results:The results showed that all the plant parts possessed antioxidant properties including radical scavenging,xanthine oxidase ildfibition and nitrites seavenging activities.The antioxidative activities were correlated with the total phenol.The leaves extract of L.camara was more effective than that of other parts.Conclusions:This study suggests that L.camara extracts exhibit great potential for antioxidant activity and may be useful for their nutdtional and medicinal functions.