Objective:We investigated the effects of exogenous AP-2α gene on SW620 cell cycle, apoptosis and proliferation. Methods:The Plasmid pcDNA3.1(+)-AP-2α was transfected into colorectal carcinoma SW620 cells line by lip...Objective:We investigated the effects of exogenous AP-2α gene on SW620 cell cycle, apoptosis and proliferation. Methods:The Plasmid pcDNA3.1(+)-AP-2α was transfected into colorectal carcinoma SW620 cells line by liposome mediation for transient expression. After AP-2α transfected SW620 cells, the exogenous AP-2α mRNA and protein express were determined by the method of Real-time PCR and Western blot. In order to elucidate the effect of expression of exogenous AP-2α gene on the colorectal cancer cell SW620, the proliferation rates were analyzed by growth curves for cells including SW620, pcDNA3.1(+)/SW620 and pcDNA3.1(+)-AP-2α/SW620. At same time, the apoptotic rate of cells and the cell cycle analysis were also tested by flow cytometry. Results: The mRNA and protein expressions of AP-2α gene could be enhanced by transfecting of pcDNA3.1(+)-AP-2α recombinant plasmid into SW620 cell. The cell growth rates of SW620 cells transfected with pcDNA3.1(+)/AP-2α were slower than those transfected with pcDNA3.1(+) or untransfected. The apoptotic rates were increased compared with pcDNA3.1(+)/SW620 and SW620 (P<0.05), which indicated that over-expression of AP-2α gene could efficiently inhibit the growth of SW620 cells and induce apoptosis. FCM analyses indicated that the cells being arrested in G1 phase. Conclusion: AP-2α gene can be efficiently transfected into SW620 cells and over-expression AP-2α protein in the transfected cells. AP-2α induces G1 arrest and apoptosis, suppressive effect on cell growth.展开更多
MicroRNAs (miRNAs) constitute a class of small non-coding RNAs that function as posttranscriptional gene regulators. The dysregulation of miRNAs has been linked to a series of diseases, including various types of canc...MicroRNAs (miRNAs) constitute a class of small non-coding RNAs that function as posttranscriptional gene regulators. The dysregulation of miRNAs has been linked to a series of diseases, including various types of cancer. Since their discovery in the miRNAs of cancer patients, there has been a steady increase in the study of miRNAs as stable, noninvasive biomarkers. Although several challenges remain to be concerned, miRNAs could be useful, non-invasive biomarkers for colorectal cancer diagnostics and prognosis. In this review, we summarized the discovery of miRNAs and their potential as biomarkers. We discussed their possible structural, function and further emphasized the significance of miRNAs in colorectal cancer (CRC).展开更多
The mechanical properties of nano-scale Cu/FeS composite were simulated by molecular dynamics (MD) simulation in this paper. Through the analysis on the stress-strain curves, the results of MD simulation were in goo...The mechanical properties of nano-scale Cu/FeS composite were simulated by molecular dynamics (MD) simulation in this paper. Through the analysis on the stress-strain curves, the results of MD simulation were in good agreement with mechanisms of macroscopic deformation. When the size of particles was smaller than a certain value, the relationship between yield strength and size, which was different from the large size crystals abided by the contrary Hall-Petch relationship. Based on the discussion of nano-scale Cu/FeS composite, some interesting conclusions were obtained. For example, the "S" type curves were discovered in stress-strain curves and the anisotropy of FeS was very evident when the exposures of reinforcing phase (FeS) were different and so on. The basic theories and calculations of the composite that contains nano-scale particles were discussed. At the same time, a new modeling building method of composites, which was close to actual experiences, were considered in this paper.展开更多
文摘Objective:We investigated the effects of exogenous AP-2α gene on SW620 cell cycle, apoptosis and proliferation. Methods:The Plasmid pcDNA3.1(+)-AP-2α was transfected into colorectal carcinoma SW620 cells line by liposome mediation for transient expression. After AP-2α transfected SW620 cells, the exogenous AP-2α mRNA and protein express were determined by the method of Real-time PCR and Western blot. In order to elucidate the effect of expression of exogenous AP-2α gene on the colorectal cancer cell SW620, the proliferation rates were analyzed by growth curves for cells including SW620, pcDNA3.1(+)/SW620 and pcDNA3.1(+)-AP-2α/SW620. At same time, the apoptotic rate of cells and the cell cycle analysis were also tested by flow cytometry. Results: The mRNA and protein expressions of AP-2α gene could be enhanced by transfecting of pcDNA3.1(+)-AP-2α recombinant plasmid into SW620 cell. The cell growth rates of SW620 cells transfected with pcDNA3.1(+)/AP-2α were slower than those transfected with pcDNA3.1(+) or untransfected. The apoptotic rates were increased compared with pcDNA3.1(+)/SW620 and SW620 (P<0.05), which indicated that over-expression of AP-2α gene could efficiently inhibit the growth of SW620 cells and induce apoptosis. FCM analyses indicated that the cells being arrested in G1 phase. Conclusion: AP-2α gene can be efficiently transfected into SW620 cells and over-expression AP-2α protein in the transfected cells. AP-2α induces G1 arrest and apoptosis, suppressive effect on cell growth.
文摘MicroRNAs (miRNAs) constitute a class of small non-coding RNAs that function as posttranscriptional gene regulators. The dysregulation of miRNAs has been linked to a series of diseases, including various types of cancer. Since their discovery in the miRNAs of cancer patients, there has been a steady increase in the study of miRNAs as stable, noninvasive biomarkers. Although several challenges remain to be concerned, miRNAs could be useful, non-invasive biomarkers for colorectal cancer diagnostics and prognosis. In this review, we summarized the discovery of miRNAs and their potential as biomarkers. We discussed their possible structural, function and further emphasized the significance of miRNAs in colorectal cancer (CRC).
基金supported by the National Basic Research Program of China (973 Program) under Grant No. 2008CB617609the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China under Grant No.2007BAE23B02
文摘The mechanical properties of nano-scale Cu/FeS composite were simulated by molecular dynamics (MD) simulation in this paper. Through the analysis on the stress-strain curves, the results of MD simulation were in good agreement with mechanisms of macroscopic deformation. When the size of particles was smaller than a certain value, the relationship between yield strength and size, which was different from the large size crystals abided by the contrary Hall-Petch relationship. Based on the discussion of nano-scale Cu/FeS composite, some interesting conclusions were obtained. For example, the "S" type curves were discovered in stress-strain curves and the anisotropy of FeS was very evident when the exposures of reinforcing phase (FeS) were different and so on. The basic theories and calculations of the composite that contains nano-scale particles were discussed. At the same time, a new modeling building method of composites, which was close to actual experiences, were considered in this paper.