In this Letter, we present a high-speed volumetric imaging system based on structured illumination and an electrically tunable lens（ETL）, where the ETL performs fast axial scanning at hundreds of Hz. In the system,a...In this Letter, we present a high-speed volumetric imaging system based on structured illumination and an electrically tunable lens（ETL）, where the ETL performs fast axial scanning at hundreds of Hz. In the system,a digital micro-mirror device（DMD） is utilized to rapidly generate structured images at the focal plane in synchronization with the axial scanning unit. The scanning characteristics of the ETL are investigated theoretically and experimentally. Imaging experiments on pollen samples are performed to verify the optical cross-sectioning and fast axial scanning capabilities. The results show that our system can perform fast axial scanning and threedimensional（3D） imaging when paired with a high-speed camera, presenting an economic solution for advanced biological imaging applications.展开更多
基金supported by the National Natural Science Foundation of China(NSFC),General Program(No.51375415)the Development of a Flexure-based Optical Scanning System and a Multimodal Nonlinear Endomicroscope for in vivo Biological Studiesthe HKSAR Research Grants Council(RGC)General Research Fund(CUHK 14202815)
文摘In this Letter, we present a high-speed volumetric imaging system based on structured illumination and an electrically tunable lens（ETL）, where the ETL performs fast axial scanning at hundreds of Hz. In the system,a digital micro-mirror device（DMD） is utilized to rapidly generate structured images at the focal plane in synchronization with the axial scanning unit. The scanning characteristics of the ETL are investigated theoretically and experimentally. Imaging experiments on pollen samples are performed to verify the optical cross-sectioning and fast axial scanning capabilities. The results show that our system can perform fast axial scanning and threedimensional（3D） imaging when paired with a high-speed camera, presenting an economic solution for advanced biological imaging applications.
