The WRKY gene family is most widely known as being the key plant transcription factor family involved in various stress responses and affecting plant growth and development.In this study,a total of 86 members of the C...The WRKY gene family is most widely known as being the key plant transcription factor family involved in various stress responses and affecting plant growth and development.In this study,a total of 86 members of the CsWRKY genes were identified from the tea plant genome.Most of these genes contain several important Cis-regulatory elements in the promoter regions associated with multiple stress-responses.These genes were further classified into three groups,I,II,and III,each with 21,58,and 7 members,respectively.We showed evidence that tandem duplications,but not the whole genome duplication,are likely to drive the amplification of CsWRKY genes in tea plants.All the 86 CsWRKY genes showed differential expression patterns either in different tissues,or under exposure to diverse abiotic stresses such as drought,cold acclimation,and MeJA treatments.Additionally,the functional roles of two genes,CsWRKY29 and CsWRKY37,were examined under cold stress;and the silencing of these genes resulted in tea plant phenotypes susceptible to cold stress.Moreover,transgenic Arabidopsis lines overexpressing CsWRKY29 and CsWRKY37 genes showed higher survival rates and lower malondialdehyde levels under freezing treatment than the wild type plants.The core findings from this work provide valuable evolutionary pattern of WRKY gene family and underpinning the underlying regulatory roles of CsWRKY29 and CsWRKY37 from tea plants that conferred cold tolerance in transgenic Arabidopsis plants.展开更多
Tea plants grow in acidic soil, but to date, their intrinsic mechanisms of acidic stress tolerance have not been elucidated. Here, we assessed the tea plant response to growth on NHt4 nutrient media having different p...Tea plants grow in acidic soil, but to date, their intrinsic mechanisms of acidic stress tolerance have not been elucidated. Here, we assessed the tea plant response to growth on NHt4 nutrient media having different p H and iron levels. When grown in standard NHt4 nutrient solution(iron insufficient, 0.35 mg Là1 Fe2t), tea roots exhibited significantly lower nitrogen accumulation, plasma membrane Ht-ATPase activity, and protein levels; net Htefflux was lower at pH 4.0 and 5.0 than at pH 6.0. Addition of30 mg Là1 Fe2t(iron sufficient, mimicking normal soil Fe2tconcentrations) to the NHt4 nutrient solution led to more efficient iron plaque formation on roots and increased root plasma membrane Ht-ATPase levels and activities at p H 4.0 eland 5.0, compared to the p H 6.0 condition. Furthermore,plants grown at pH 4.0 and 5.0, with sufficient iron,exhibited significantly higher nitrogen accumulation than those grown at pH 6.0. Together, these results support the hypothesis that efficient iron plaque formation, on tea roots, is important for acidic stress tolerance. Furthermore,our findings establish that efficient iron plaque formation is linked to increased levels and activities of the tea root plasma membrane Ht-ATPase, under low pH conditions.展开更多
基金the National Natural Science Foundation of China(32172626)the Anhui Provincial Natural Science Foundation(2208085MC72,1908085MC75)the Anhui University Collaborative Innovation Project(GXXT-2020-080).
文摘The WRKY gene family is most widely known as being the key plant transcription factor family involved in various stress responses and affecting plant growth and development.In this study,a total of 86 members of the CsWRKY genes were identified from the tea plant genome.Most of these genes contain several important Cis-regulatory elements in the promoter regions associated with multiple stress-responses.These genes were further classified into three groups,I,II,and III,each with 21,58,and 7 members,respectively.We showed evidence that tandem duplications,but not the whole genome duplication,are likely to drive the amplification of CsWRKY genes in tea plants.All the 86 CsWRKY genes showed differential expression patterns either in different tissues,or under exposure to diverse abiotic stresses such as drought,cold acclimation,and MeJA treatments.Additionally,the functional roles of two genes,CsWRKY29 and CsWRKY37,were examined under cold stress;and the silencing of these genes resulted in tea plant phenotypes susceptible to cold stress.Moreover,transgenic Arabidopsis lines overexpressing CsWRKY29 and CsWRKY37 genes showed higher survival rates and lower malondialdehyde levels under freezing treatment than the wild type plants.The core findings from this work provide valuable evolutionary pattern of WRKY gene family and underpinning the underlying regulatory roles of CsWRKY29 and CsWRKY37 from tea plants that conferred cold tolerance in transgenic Arabidopsis plants.
基金primarily supported by the Science Foundation for Anhui Province (KJ2017A126) to Z.X.the Open Fund of State Key Laboratory of Tea Plant Biology and Utilization at Anhui Agricultural University (SKLTOF20170112) to W.H.and Z.X.+1 种基金supported by the National Natural Science Foundation of China (grant number 11008389)the National Key Basic Research and Development Project (973) (11000206) to X.W
文摘Tea plants grow in acidic soil, but to date, their intrinsic mechanisms of acidic stress tolerance have not been elucidated. Here, we assessed the tea plant response to growth on NHt4 nutrient media having different p H and iron levels. When grown in standard NHt4 nutrient solution(iron insufficient, 0.35 mg Là1 Fe2t), tea roots exhibited significantly lower nitrogen accumulation, plasma membrane Ht-ATPase activity, and protein levels; net Htefflux was lower at pH 4.0 and 5.0 than at pH 6.0. Addition of30 mg Là1 Fe2t(iron sufficient, mimicking normal soil Fe2tconcentrations) to the NHt4 nutrient solution led to more efficient iron plaque formation on roots and increased root plasma membrane Ht-ATPase levels and activities at p H 4.0 eland 5.0, compared to the p H 6.0 condition. Furthermore,plants grown at pH 4.0 and 5.0, with sufficient iron,exhibited significantly higher nitrogen accumulation than those grown at pH 6.0. Together, these results support the hypothesis that efficient iron plaque formation, on tea roots, is important for acidic stress tolerance. Furthermore,our findings establish that efficient iron plaque formation is linked to increased levels and activities of the tea root plasma membrane Ht-ATPase, under low pH conditions.