BACKGROUND The prognosis of gastric cancer is extremely poor.Metabolic reprogramming involving lipids has been associated with cancer occurrence and progression.AIM To illustrate fatty acid metabolic mechanisms in gas...BACKGROUND The prognosis of gastric cancer is extremely poor.Metabolic reprogramming involving lipids has been associated with cancer occurrence and progression.AIM To illustrate fatty acid metabolic mechanisms in gastric cancer,detect core genes,develop a prognostic model,and provide treatment options.METHODS Raw data from The Cancer Genome Atlas and Gene Expression Omnibus databases were collected and analyzed.Differentially expressed fatty acid metabolism genes were identified and incorporated into a risk model based on least absolute shrinkage and selection operator regression analysis.Then,patients from The Cancer Genome Atlas were assigned to high-and low-risk cohorts according to the mean value of the risk score as the threshold,which was verified in the Gene Expression Omnibus database.Relationships between chemotherapeutic sensitivity and tumor microenvironment features were assessed.RESULTS An integrated evaluation was performed in this study.Fatty acid metabolismrelated genes were used to construct the risk model.Patients classified into the high-risk cohort were considered to be resistant to chemotherapy based on results of the“pRRophetic”R package.Patients in the high-risk cohort were associated with type Ⅰ/Ⅱ interferon activation,increased inflammation level,immune cell infiltration,and tumor immune dysfunction based on the exclusion algorithm,indicating the potential benefit of immunotherapy in these patients.CONCLUSION We constructed a fatty acid-related risk score model to assess the comprehensive fatty acid features in gastric cancer and validated its vital role in prognosis,chemotherapy sensitivity,and immunotherapy.展开更多
Alginate oligosaccharides(AOS), belonging to the class of functional marine oligosaccharides, are low-molecular polymers linked by β-1,4-mannuronic acid(M) and α-1,4-guluronic acid(G), which could be classically obt...Alginate oligosaccharides(AOS), belonging to the class of functional marine oligosaccharides, are low-molecular polymers linked by β-1,4-mannuronic acid(M) and α-1,4-guluronic acid(G), which could be classically obtained by enzymatic hydrolysis of alginate. With low viscosity and good water solubility, as well as anti-oxidant, immune regulation, anti-bacterial and antiinflammatory activities, AOS have been widely used in medical science and functional food, green agriculture and other fields. As new bio-feed additives, AOS have broad potential applications in animal husbandry. In this review, the sources of alginate, chemical structure and preparation methods of AOS, and their biological activities and application in livestock and poultry are summarized. We expect this review could contribute to lay a foundation of application and further research for AOS in livestock and poultry.展开更多
NEOPLASMS derive from small intestine are rareand most cases are adenocarcinomas andcarcinoid.1 Squamous cell carcinoma of smallintestine is even rarer and only few casesreported in literature.1 In this article, we re...NEOPLASMS derive from small intestine are rareand most cases are adenocarcinomas andcarcinoid.1 Squamous cell carcinoma of smallintestine is even rarer and only few casesreported in literature.1 In this article, we report a case of a68-year-old male who underwent a laparotomy due toperforation of the small intestine and was diagnosed withsquamous cell carcinoma of the small intestine.展开更多
Objective:Complex ureteral obstruction is refractory to conventional urological intervention.This report describes a case of laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for tr...Objective:Complex ureteral obstruction is refractory to conventional urological intervention.This report describes a case of laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for treating complex ureteral obstruction.Methods:Right-side multiple ureteral stones and complicating ureteral obstruction failed an initial attempt of ureteroscopy lithotripsy with simultaneous percutaneous nephroscopy in a 23-year-old male.Laparoscopic ureterolysis with ureteroscopy and percutaneous nephroscopy was used simultaneously to dissect the periureteral adhesions with the patient placed in the Galdakao-modified supine Valdivia position.The ureter was incised to allow the insertion of a ureteral catheter through the twisted ureter,and a guide wire was advanced into the pelvis using ureteroscopy.A double-J stent was placed into the right-side ureter using antegrade percutaneous nephroscopy.Results:The laparoendoscopic procedure lasted 330 min with an estimated bleeding volume of 100 mL.The patient underwent an uneventful postoperative course,and postoperative followup radiography confirmed good positioning of the double-J stent.The double-J stent was removed 3 months after operation.The patient remained asymptomatic within a 13-month follow-up period.Conclusion:Laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy is an effective and safe treatment option for complex ureteral obstruction.展开更多
Objective To investigate trichorhinophalangeal syndrome 1 gene(TRPS-1)expression patterns in different subtypes of breast cancer and its correlations with other genes and survival using microarray data sets.Methods Th...Objective To investigate trichorhinophalangeal syndrome 1 gene(TRPS-1)expression patterns in different subtypes of breast cancer and its correlations with other genes and survival using microarray data sets.Methods The transcripts of TRPS-1 and its role in survival in breast cancer were analyzed using published microarray data sets—Netherlands Cancer Institute(NKI)cohort and Wang cohort.Results TRPS-1 expression was lower in basal-like breast cancer.The mRNA levels of TRPS-1negatively correlated with Slug(Pearson correlation coefficient=0.1366,P=0.0189 in NKI data set and Pearson correlation coefficient=0.1571,P=0.0078 in Wang data set),FOXC1(Pearson correlation coefficient=0.1211,P=0.0376 in NKI data set and Pearson correlation coefficient=0.1709,P=0.0037 in Wang data set),and CXCL1(Pearson correlation coefficient=0.1197,P=0.0399 in NKI data set and Pearson correlation coefficient=0.3436,P<0.0001 in Wang data set),but positively correlated with BRCA1(Pearson correlation coefficient=0.1728,P=0.0029 in NKI data set and Pearson correlation coefficient=0.1805,P=0.0022 in Wang data set).Low TRPS-1 expression associated with poor overall survival(hazard ratio 1.79,95%CI of ratio 0.9894 to 3.238,P=0.054)and relapse-free survival(hazard ratio 1.913,95%CI of ratio1.159 to 3.156,P<0.05).The low TRPS-1 mRNA levels predicted poor outcome in breast cancer patients by the 70-gene signature.Conclusion The strong expression of TRPS-1 may serve as a good prognostic marker in breast cancer.展开更多
文摘BACKGROUND The prognosis of gastric cancer is extremely poor.Metabolic reprogramming involving lipids has been associated with cancer occurrence and progression.AIM To illustrate fatty acid metabolic mechanisms in gastric cancer,detect core genes,develop a prognostic model,and provide treatment options.METHODS Raw data from The Cancer Genome Atlas and Gene Expression Omnibus databases were collected and analyzed.Differentially expressed fatty acid metabolism genes were identified and incorporated into a risk model based on least absolute shrinkage and selection operator regression analysis.Then,patients from The Cancer Genome Atlas were assigned to high-and low-risk cohorts according to the mean value of the risk score as the threshold,which was verified in the Gene Expression Omnibus database.Relationships between chemotherapeutic sensitivity and tumor microenvironment features were assessed.RESULTS An integrated evaluation was performed in this study.Fatty acid metabolismrelated genes were used to construct the risk model.Patients classified into the high-risk cohort were considered to be resistant to chemotherapy based on results of the“pRRophetic”R package.Patients in the high-risk cohort were associated with type Ⅰ/Ⅱ interferon activation,increased inflammation level,immune cell infiltration,and tumor immune dysfunction based on the exclusion algorithm,indicating the potential benefit of immunotherapy in these patients.CONCLUSION We constructed a fatty acid-related risk score model to assess the comprehensive fatty acid features in gastric cancer and validated its vital role in prognosis,chemotherapy sensitivity,and immunotherapy.
基金the National Key R&D Program of China (2016YFD0500501)the National Natural Science Foundation of China (31772641)the Basic Science and Research Funding of Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, China (2018-YWF-YB-11)。
文摘Alginate oligosaccharides(AOS), belonging to the class of functional marine oligosaccharides, are low-molecular polymers linked by β-1,4-mannuronic acid(M) and α-1,4-guluronic acid(G), which could be classically obtained by enzymatic hydrolysis of alginate. With low viscosity and good water solubility, as well as anti-oxidant, immune regulation, anti-bacterial and antiinflammatory activities, AOS have been widely used in medical science and functional food, green agriculture and other fields. As new bio-feed additives, AOS have broad potential applications in animal husbandry. In this review, the sources of alginate, chemical structure and preparation methods of AOS, and their biological activities and application in livestock and poultry are summarized. We expect this review could contribute to lay a foundation of application and further research for AOS in livestock and poultry.
基金Supported by Young Scientists Fund of National Natural Science Foundation of China(81101707)Zhejiang Traditional Chinese Medicine Foundation Project(2014ZB119)Science and Technology Bureau of Jiaxing(2012AY1071-2)
文摘NEOPLASMS derive from small intestine are rareand most cases are adenocarcinomas andcarcinoid.1 Squamous cell carcinoma of smallintestine is even rarer and only few casesreported in literature.1 In this article, we report a case of a68-year-old male who underwent a laparotomy due toperforation of the small intestine and was diagnosed withsquamous cell carcinoma of the small intestine.
基金supported by grants from the Shanghai Municipal Hospitals’Project for Emerging and Frontier Technology(No.SHDC12010115)Chinese Military Major Project for Clinical High-tech and Innovative Technology(No.2010gxjs057)the Project for the Key Discipline of Shanghai(No.2013046).
文摘Objective:Complex ureteral obstruction is refractory to conventional urological intervention.This report describes a case of laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy for treating complex ureteral obstruction.Methods:Right-side multiple ureteral stones and complicating ureteral obstruction failed an initial attempt of ureteroscopy lithotripsy with simultaneous percutaneous nephroscopy in a 23-year-old male.Laparoscopic ureterolysis with ureteroscopy and percutaneous nephroscopy was used simultaneously to dissect the periureteral adhesions with the patient placed in the Galdakao-modified supine Valdivia position.The ureter was incised to allow the insertion of a ureteral catheter through the twisted ureter,and a guide wire was advanced into the pelvis using ureteroscopy.A double-J stent was placed into the right-side ureter using antegrade percutaneous nephroscopy.Results:The laparoendoscopic procedure lasted 330 min with an estimated bleeding volume of 100 mL.The patient underwent an uneventful postoperative course,and postoperative followup radiography confirmed good positioning of the double-J stent.The double-J stent was removed 3 months after operation.The patient remained asymptomatic within a 13-month follow-up period.Conclusion:Laparoscopic ureterolysis with simultaneous ureteroscopy and percutaneous nephroscopy is an effective and safe treatment option for complex ureteral obstruction.
基金Supported by grants from Chinese National Science Fund for Young Scholars(81101707)Traditional Chinese Medicine Foundation Project of Zhejiang Province(2011ZA104)Science and Technology Bureau of Jiaxing(2012AY1071-2)
文摘Objective To investigate trichorhinophalangeal syndrome 1 gene(TRPS-1)expression patterns in different subtypes of breast cancer and its correlations with other genes and survival using microarray data sets.Methods The transcripts of TRPS-1 and its role in survival in breast cancer were analyzed using published microarray data sets—Netherlands Cancer Institute(NKI)cohort and Wang cohort.Results TRPS-1 expression was lower in basal-like breast cancer.The mRNA levels of TRPS-1negatively correlated with Slug(Pearson correlation coefficient=0.1366,P=0.0189 in NKI data set and Pearson correlation coefficient=0.1571,P=0.0078 in Wang data set),FOXC1(Pearson correlation coefficient=0.1211,P=0.0376 in NKI data set and Pearson correlation coefficient=0.1709,P=0.0037 in Wang data set),and CXCL1(Pearson correlation coefficient=0.1197,P=0.0399 in NKI data set and Pearson correlation coefficient=0.3436,P<0.0001 in Wang data set),but positively correlated with BRCA1(Pearson correlation coefficient=0.1728,P=0.0029 in NKI data set and Pearson correlation coefficient=0.1805,P=0.0022 in Wang data set).Low TRPS-1 expression associated with poor overall survival(hazard ratio 1.79,95%CI of ratio 0.9894 to 3.238,P=0.054)and relapse-free survival(hazard ratio 1.913,95%CI of ratio1.159 to 3.156,P<0.05).The low TRPS-1 mRNA levels predicted poor outcome in breast cancer patients by the 70-gene signature.Conclusion The strong expression of TRPS-1 may serve as a good prognostic marker in breast cancer.
