Objective: Previous studies have shown that glioma cells induced the migration of nerve stem cells (NSCs) in vivo. This study was exploring whether this situation could happen in vitro. Methods: Supematant from 05...Objective: Previous studies have shown that glioma cells induced the migration of nerve stem cells (NSCs) in vivo. This study was exploring whether this situation could happen in vitro. Methods: Supematant from 05 glioma cell lines or astrocytes cultured in serum-free medium growing in logarithmic phase were separately placed in lower chambers and NSCs were placed in the upper chambers of Trans-well culture system. After 36 h co-incubation, these NSCs spheres occurred on the middle membrane were counted. Results: Results demonstrated that the supematant from 05 glioma cell culture, not from the astrocytes, enhanced the migration of NSCs ( P 〈0.01). Conclusion: Some components in 05 glioma cell culture can attract the migration of NSCs.展开更多
马克思·约瑟夫·冯·皮腾科菲尔(Max Joseph von Pettenkofer,1818-1901),19世纪德国杰出的化学家和卫生科学家,专攻环境卫生与传染病学研究。他极力反对疾病细菌学说,认为疾病发生和流行与外在环境的失调、紊乱有不可忽...马克思·约瑟夫·冯·皮腾科菲尔(Max Joseph von Pettenkofer,1818-1901),19世纪德国杰出的化学家和卫生科学家,专攻环境卫生与传染病学研究。他极力反对疾病细菌学说,认为疾病发生和流行与外在环境的失调、紊乱有不可忽视的关系。通过研究环境因素与霍乱的关系,他提出了霍乱理论。此外,他创立了世界上第一个卫生科学专业,提出了很多预防传染病的方法,在传染病预防上做出了巨大贡献。他是现代卫生科学开拓者和先驱者之一,被誉为现代卫生科学的奠基人。展开更多
文摘Objective: Previous studies have shown that glioma cells induced the migration of nerve stem cells (NSCs) in vivo. This study was exploring whether this situation could happen in vitro. Methods: Supematant from 05 glioma cell lines or astrocytes cultured in serum-free medium growing in logarithmic phase were separately placed in lower chambers and NSCs were placed in the upper chambers of Trans-well culture system. After 36 h co-incubation, these NSCs spheres occurred on the middle membrane were counted. Results: Results demonstrated that the supematant from 05 glioma cell culture, not from the astrocytes, enhanced the migration of NSCs ( P 〈0.01). Conclusion: Some components in 05 glioma cell culture can attract the migration of NSCs.
文摘马克思·约瑟夫·冯·皮腾科菲尔(Max Joseph von Pettenkofer,1818-1901),19世纪德国杰出的化学家和卫生科学家,专攻环境卫生与传染病学研究。他极力反对疾病细菌学说,认为疾病发生和流行与外在环境的失调、紊乱有不可忽视的关系。通过研究环境因素与霍乱的关系,他提出了霍乱理论。此外,他创立了世界上第一个卫生科学专业,提出了很多预防传染病的方法,在传染病预防上做出了巨大贡献。他是现代卫生科学开拓者和先驱者之一,被誉为现代卫生科学的奠基人。