Neurons can be abstractly represented as skeletons due to the filament nature of neurites.With the rapid development of imaging and image analysis techniques,an increasing amount of neuron skeleton data is being produ...Neurons can be abstractly represented as skeletons due to the filament nature of neurites.With the rapid development of imaging and image analysis techniques,an increasing amount of neuron skeleton data is being produced.In some scienti fic studies,it is necessary to dissect the axons and dendrites,which is typically done manually and is both tedious and time-consuming.To automate this process,we have developed a method that relies solely on neuronal skeletons using Geometric Deep Learning(GDL).We demonstrate the effectiveness of this method using pyramidal neurons in mammalian brains,and the results are promising for its application in neuroscience studies.展开更多
Loop-mediated isothermal ampliifcation （LAMP） is a novel nucleic acid diagnostic method that can amplify rapidly a target template under isothermal conditions. In this study, a LAMP assay for rapid detection of Chin...Loop-mediated isothermal ampliifcation （LAMP） is a novel nucleic acid diagnostic method that can amplify rapidly a target template under isothermal conditions. In this study, a LAMP assay for rapid detection of Chinese giant salamander ranavirus（CGSRV） was developed from culture isolates and clinical samples. The LAMP assay was developed by designing one set of four speciifc primers, targeting the major capsid protein （MCP） gene of CGSRV. Reaction time and temperature were optimal for 40 min at 62°C. The developed LAMP assay is speciifc and highly sensitive for CGSRV detection, the detection limit could reach about 5 copies of cloned viral genomic fragments. The sensitivity of the LAMP assay was about 1000 and 10-fold higher than that of both conventional and nested PCR, respectively. The LAMP ampliifcation produces a typical ladder-like pattern of products on an agarose gel that can be visually inspected after addition of ethidium bromide. The LAMP assay was evaluated further with clinical samples, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for the detection of CGSRV.展开更多
Chinese giant salamander ranavirus （CGSRV） is an emerging pathogen in captive populations of the Chinese giant salamander （Andrias davidianus）. We processed 140 morbid Chinese giant salamanders from seven captive ...Chinese giant salamander ranavirus （CGSRV） is an emerging pathogen in captive populations of the Chinese giant salamander （Andrias davidianus）. We processed 140 morbid Chinese giant salamanders from seven captive breeding populations over five years, and describe the disease associated with CGSRV infection. The most common gross signs were significant swelling of the legs and coelomic cavity, erythema of the legs and ventrum in juveniles; cutaneous erosions and ulcerations in adults, particularly the limbs and the head; and marked petechial or ecchymotic hemorrhages of the internal organs, particularly the liver, spleen and kidney. Histological examination showed degeneration, necrosis, and inflammation in many organs, particularly in the organs where hemorrhage was observed. There was evidence of eosinophilic inclusion bodies in degenerated and necrotic cells. We identified virus particles and empty capsids without viral nucleoid in the inclusion bodies using electron microscopy. Virus particles were hexagonal or round shape, and appeared in paracrystalline arrays, aggregates, or singly. All enveloped viral particles were 140-160 nm. Polymerase chain reaction followed by sequencing verified that the virus particles were CGSRV. These results collectively support that CGSRV was the etiologic agent responsible for these mass die-offs of the Chinese giant salamander. The pathology described herein will be useful in diagnosing cases of ranaviral disease caused by CGSRV, and provide evidence that this pathogen is a significant threat to the Chinese giant salamander.展开更多
Introduction:Granuloma annulare(GA)is a benign,noninfectious granulomatous disorder of unknown etiology,characterized by an annular arrangement of erythematous small nodules and plaques,classically localized on dorsal...Introduction:Granuloma annulare(GA)is a benign,noninfectious granulomatous disorder of unknown etiology,characterized by an annular arrangement of erythematous small nodules and plaques,classically localized on dorsal surfaces of the hands and feet.GA shows palisading granulomas with a central zone of necrobiotic collagen and mucin deposition surrounded by a palisade of histiocytes.Here,we reported a 24-year-old man with GA combined with tuberculosis and cutis laxa.Case presentation:A 24-year-old man was presented with skin annular lesions on the trunk and extremities without subjective symptoms for three years.The annular lesions first arose in the trunk that slowly progressed to most parts of the body and cutis laxa-like lesions appeared on the left inguinal region with inguinal hernia two years ago.At the same time,the patient was diagnosed with pulmonary tuberculosis due to the hemoptysis for two years and the sputum culture for tuberculosis bacillus was positive.He was treated with triple anti-tuberculosis drugs regularly.Partial remission of the skin lesions was noted since then.Pathological examination showed similar histological changes in the interstitial pattern of GA.Discussion:Tuberculosis was a possible etiologic factor in GA because of the coincidental occurrence,granulomatous histologic features,and positive tuberculin skin tests.In fact,some cases of GA with chronic pulmonary tuberculosis responding to a specific anti-tuberculosis therapy were reported.It is worth noting that tuberculids,generalized exanthems in the skin of tuberculous patients possibly resulting from hypersensitivity reactions to the tubercle bacillus,may mimic GA.Furthermore,our patient also showed extremely characteristic slack skin in posterior lumbar and groin,which was deemed to be related to reduction and destruction of elastic fibers.Conclusion:Generalized interstitial GA with tuberculosis and cutis laxa is vary rare,which is worth being aware of the possibility of such a occurrence.展开更多
Traditional one-way imaging methods become invalid when a target object is completely hidden behind scattering media. In this case, it has been much more challenging, since the light wave is distorted twice.To solve t...Traditional one-way imaging methods become invalid when a target object is completely hidden behind scattering media. In this case, it has been much more challenging, since the light wave is distorted twice.To solve this problem, we propose an imaging method, so-called round-trip imaging, based on the optical transmission matrix of the scattering medium. We show that the object can be recovered directly from the distorted output wave, where no scanning is required during the imaging process. We predict that this method might improve the imaging speed and have potential application for real-time imaging.展开更多
Introduction Mycosis fungoides (MF) is the most common form of cutaneous lymphoma,accounting for almost 50% of all types.1 MF occasionally manifests as the interstitial lymphocytic infiltrate that mimics inflammatory ...Introduction Mycosis fungoides (MF) is the most common form of cutaneous lymphoma,accounting for almost 50% of all types.1 MF occasionally manifests as the interstitial lymphocytic infiltrate that mimics inflammatory morphea,interstitial granulomatous dermatitis,and interstitial granuloma annulare,and this rare histopathological variant has been termed interstitial mycosis fungoides (IMF).IMF can be considered as a transient histopathological pattern in conventional MF,with common clinical presentation as patches and plaques.展开更多
基金supported by the Simons Foundation,the National Natural Science Foundation of China(No.NSFC61405038)the Fujian provincial fund(No.2020J01453).
文摘Neurons can be abstractly represented as skeletons due to the filament nature of neurites.With the rapid development of imaging and image analysis techniques,an increasing amount of neuron skeleton data is being produced.In some scienti fic studies,it is necessary to dissect the axons and dendrites,which is typically done manually and is both tedious and time-consuming.To automate this process,we have developed a method that relies solely on neuronal skeletons using Geometric Deep Learning(GDL).We demonstrate the effectiveness of this method using pyramidal neurons in mammalian brains,and the results are promising for its application in neuroscience studies.
基金supported by the Sichuan Technology Support Planning (No. 2014 NZ0027)
文摘Loop-mediated isothermal ampliifcation （LAMP） is a novel nucleic acid diagnostic method that can amplify rapidly a target template under isothermal conditions. In this study, a LAMP assay for rapid detection of Chinese giant salamander ranavirus（CGSRV） was developed from culture isolates and clinical samples. The LAMP assay was developed by designing one set of four speciifc primers, targeting the major capsid protein （MCP） gene of CGSRV. Reaction time and temperature were optimal for 40 min at 62°C. The developed LAMP assay is speciifc and highly sensitive for CGSRV detection, the detection limit could reach about 5 copies of cloned viral genomic fragments. The sensitivity of the LAMP assay was about 1000 and 10-fold higher than that of both conventional and nested PCR, respectively. The LAMP ampliifcation produces a typical ladder-like pattern of products on an agarose gel that can be visually inspected after addition of ethidium bromide. The LAMP assay was evaluated further with clinical samples, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for the detection of CGSRV.
基金supported by the Sichuan Technology Support Planning (No.2014NZ0027)Sichuan Academic Leader Training Fund (No.2015RST0016)
文摘Chinese giant salamander ranavirus （CGSRV） is an emerging pathogen in captive populations of the Chinese giant salamander （Andrias davidianus）. We processed 140 morbid Chinese giant salamanders from seven captive breeding populations over five years, and describe the disease associated with CGSRV infection. The most common gross signs were significant swelling of the legs and coelomic cavity, erythema of the legs and ventrum in juveniles; cutaneous erosions and ulcerations in adults, particularly the limbs and the head; and marked petechial or ecchymotic hemorrhages of the internal organs, particularly the liver, spleen and kidney. Histological examination showed degeneration, necrosis, and inflammation in many organs, particularly in the organs where hemorrhage was observed. There was evidence of eosinophilic inclusion bodies in degenerated and necrotic cells. We identified virus particles and empty capsids without viral nucleoid in the inclusion bodies using electron microscopy. Virus particles were hexagonal or round shape, and appeared in paracrystalline arrays, aggregates, or singly. All enveloped viral particles were 140-160 nm. Polymerase chain reaction followed by sequencing verified that the virus particles were CGSRV. These results collectively support that CGSRV was the etiologic agent responsible for these mass die-offs of the Chinese giant salamander. The pathology described herein will be useful in diagnosing cases of ranaviral disease caused by CGSRV, and provide evidence that this pathogen is a significant threat to the Chinese giant salamander.
文摘Introduction:Granuloma annulare(GA)is a benign,noninfectious granulomatous disorder of unknown etiology,characterized by an annular arrangement of erythematous small nodules and plaques,classically localized on dorsal surfaces of the hands and feet.GA shows palisading granulomas with a central zone of necrobiotic collagen and mucin deposition surrounded by a palisade of histiocytes.Here,we reported a 24-year-old man with GA combined with tuberculosis and cutis laxa.Case presentation:A 24-year-old man was presented with skin annular lesions on the trunk and extremities without subjective symptoms for three years.The annular lesions first arose in the trunk that slowly progressed to most parts of the body and cutis laxa-like lesions appeared on the left inguinal region with inguinal hernia two years ago.At the same time,the patient was diagnosed with pulmonary tuberculosis due to the hemoptysis for two years and the sputum culture for tuberculosis bacillus was positive.He was treated with triple anti-tuberculosis drugs regularly.Partial remission of the skin lesions was noted since then.Pathological examination showed similar histological changes in the interstitial pattern of GA.Discussion:Tuberculosis was a possible etiologic factor in GA because of the coincidental occurrence,granulomatous histologic features,and positive tuberculin skin tests.In fact,some cases of GA with chronic pulmonary tuberculosis responding to a specific anti-tuberculosis therapy were reported.It is worth noting that tuberculids,generalized exanthems in the skin of tuberculous patients possibly resulting from hypersensitivity reactions to the tubercle bacillus,may mimic GA.Furthermore,our patient also showed extremely characteristic slack skin in posterior lumbar and groin,which was deemed to be related to reduction and destruction of elastic fibers.Conclusion:Generalized interstitial GA with tuberculosis and cutis laxa is vary rare,which is worth being aware of the possibility of such a occurrence.
基金supported by the National Natural Science Foundation of China(Nos.61535015,61275149,and 61275086)the Special Scientific Research Plan from Education Department of Shaanxi Provincial Government(No.16JK1083)
文摘Traditional one-way imaging methods become invalid when a target object is completely hidden behind scattering media. In this case, it has been much more challenging, since the light wave is distorted twice.To solve this problem, we propose an imaging method, so-called round-trip imaging, based on the optical transmission matrix of the scattering medium. We show that the object can be recovered directly from the distorted output wave, where no scanning is required during the imaging process. We predict that this method might improve the imaging speed and have potential application for real-time imaging.
基金supported by CAMS Innovation Fund for Medical Sciences (No. CIFMS-2017-I2M-1-107)
文摘Introduction Mycosis fungoides (MF) is the most common form of cutaneous lymphoma,accounting for almost 50% of all types.1 MF occasionally manifests as the interstitial lymphocytic infiltrate that mimics inflammatory morphea,interstitial granulomatous dermatitis,and interstitial granuloma annulare,and this rare histopathological variant has been termed interstitial mycosis fungoides (IMF).IMF can be considered as a transient histopathological pattern in conventional MF,with common clinical presentation as patches and plaques.
