研究旨在比较分析固醇调节元件结合蛋白-1(Sterol regulatory element-binding protein 1,SREBP-1)基因在湘西黄牛不同组织中的发育性表达规律,以探讨SREBP-1基因与肉牛脂肪代谢的关系。选取湖南德农牧业集团有限公司国家级保种场不同月...研究旨在比较分析固醇调节元件结合蛋白-1(Sterol regulatory element-binding protein 1,SREBP-1)基因在湘西黄牛不同组织中的发育性表达规律,以探讨SREBP-1基因与肉牛脂肪代谢的关系。选取湖南德农牧业集团有限公司国家级保种场不同月龄(6月龄、18月龄、30月龄)湘西黄牛各4头,利用实时荧光定量PCR检测肝脏、背最长肌、皮下和腹腔脂肪组织SREBP-1基因的相对表达量。结果表明:①SREBP-1基因在湘西黄牛肝脏、背最长肌、皮下和腹腔脂肪的4个部位中的表达量随月龄增加依次显著增加(P<0.01);②在6月龄时,皮下脂肪SREBP-1基因的表达量显著高于肝脏与腹腔脂肪(P<0.05);③18月龄时,SREBP-1基因在肝脏中的表达量显著高于背最长肌与腹腔脂肪(P<0.05);④30月龄时,SREBP-1基因在肝脏组织中的表达量显著高于其他3个组织(P<0.05),在皮下脂肪中的表达量显著高于背最长肌与腹腔脂肪(P<0.05)。由此可见,SREBP-1基因在湘西黄牛不同部位脂肪组织和肌肉组织中均有表达,且其表达随月龄与部位的不同而不同,存在月龄和部位的差异性。展开更多
A comparative study was conducted on four mulberry（ Morus alba） varieties（ lines） in Hunan dry land from the aspects of production performance,nutritional composition and amino acids content. The result showed tha...A comparative study was conducted on four mulberry（ Morus alba） varieties（ lines） in Hunan dry land from the aspects of production performance,nutritional composition and amino acids content. The result showed that the four mulberry varieties all adjusted well to soil,climate and other natural conditions in Hunan province,presenting good growth. The dry matter content of four varieties（ lines） was above 25% and the crude protein content was more than 22%. Although the crude protein and total amino acid content of four mulberry varieties were lower than that of soybean powder,the proportion of various amino acids tended to be consistent,so mulberry leaf was very comprehensive in nutrition as an animal feed. Yuesang 11 had the highest fresh yield and crude protein of 14. 28 t / hm^2 and23. 22% respectively; Guisangyou 12 had the highest total amino acid content of 19. 58%. There were differences among four varieties,but each variety had its own advantages,so they all had good popularization value.展开更多
文摘研究旨在比较分析固醇调节元件结合蛋白-1(Sterol regulatory element-binding protein 1,SREBP-1)基因在湘西黄牛不同组织中的发育性表达规律,以探讨SREBP-1基因与肉牛脂肪代谢的关系。选取湖南德农牧业集团有限公司国家级保种场不同月龄(6月龄、18月龄、30月龄)湘西黄牛各4头,利用实时荧光定量PCR检测肝脏、背最长肌、皮下和腹腔脂肪组织SREBP-1基因的相对表达量。结果表明:①SREBP-1基因在湘西黄牛肝脏、背最长肌、皮下和腹腔脂肪的4个部位中的表达量随月龄增加依次显著增加(P<0.01);②在6月龄时,皮下脂肪SREBP-1基因的表达量显著高于肝脏与腹腔脂肪(P<0.05);③18月龄时,SREBP-1基因在肝脏中的表达量显著高于背最长肌与腹腔脂肪(P<0.05);④30月龄时,SREBP-1基因在肝脏组织中的表达量显著高于其他3个组织(P<0.05),在皮下脂肪中的表达量显著高于背最长肌与腹腔脂肪(P<0.05)。由此可见,SREBP-1基因在湘西黄牛不同部位脂肪组织和肌肉组织中均有表达,且其表达随月龄与部位的不同而不同,存在月龄和部位的差异性。
基金Supported by Key Technology Research for Development and Efficient Cultivation and Utilization of Forage Mulberry(2013SZ03)
文摘A comparative study was conducted on four mulberry（ Morus alba） varieties（ lines） in Hunan dry land from the aspects of production performance,nutritional composition and amino acids content. The result showed that the four mulberry varieties all adjusted well to soil,climate and other natural conditions in Hunan province,presenting good growth. The dry matter content of four varieties（ lines） was above 25% and the crude protein content was more than 22%. Although the crude protein and total amino acid content of four mulberry varieties were lower than that of soybean powder,the proportion of various amino acids tended to be consistent,so mulberry leaf was very comprehensive in nutrition as an animal feed. Yuesang 11 had the highest fresh yield and crude protein of 14. 28 t / hm^2 and23. 22% respectively; Guisangyou 12 had the highest total amino acid content of 19. 58%. There were differences among four varieties,but each variety had its own advantages,so they all had good popularization value.
