Isolation and culture of ADSCs in vitro,detection of cell surface markers.Detection of alkaline phosphatase(ALP)and mineralized nodules after cell osteogenic induction.Oil red 0 staining after induction of adipogenesi...Isolation and culture of ADSCs in vitro,detection of cell surface markers.Detection of alkaline phosphatase(ALP)and mineralized nodules after cell osteogenic induction.Oil red 0 staining after induction of adipogenesis.Cells were induced to differentiate into SCs,and MTT detected cell viability;Scanning electron microscopy and immunocytochemical staining observed the expression of S100.展开更多
lncRNA GAS5(growth of arrest-speeific transeript 5)controls the occurrence,development and prognoses of cancer cells.In order to explore the association bet ween dy sregulated expression of GAS5 and patient prognosis,...lncRNA GAS5(growth of arrest-speeific transeript 5)controls the occurrence,development and prognoses of cancer cells.In order to explore the association bet ween dy sregulated expression of GAS5 and patient prognosis,we performed a meta-analysis.Data were extracted from the Databases,including PubMed,Web of Science,EMBASE,Cochrane Library,and CNBL.English literatures were cllcted on the correlation between lncRNA GAS5 expression and cancer prognostic.The retrieval time was from inception to February 2021.The meta-analysis was performed with Stata Statistical Software version 12.0.According to the inclusion and exclusion criteria,a total of 917 patients from 11 studies were included in this meta-analysis.展开更多
文摘Isolation and culture of ADSCs in vitro,detection of cell surface markers.Detection of alkaline phosphatase(ALP)and mineralized nodules after cell osteogenic induction.Oil red 0 staining after induction of adipogenesis.Cells were induced to differentiate into SCs,and MTT detected cell viability;Scanning electron microscopy and immunocytochemical staining observed the expression of S100.
文摘lncRNA GAS5(growth of arrest-speeific transeript 5)controls the occurrence,development and prognoses of cancer cells.In order to explore the association bet ween dy sregulated expression of GAS5 and patient prognosis,we performed a meta-analysis.Data were extracted from the Databases,including PubMed,Web of Science,EMBASE,Cochrane Library,and CNBL.English literatures were cllcted on the correlation between lncRNA GAS5 expression and cancer prognostic.The retrieval time was from inception to February 2021.The meta-analysis was performed with Stata Statistical Software version 12.0.According to the inclusion and exclusion criteria,a total of 917 patients from 11 studies were included in this meta-analysis.