Prevention and Treatment Strategy of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome(HP-PRRS)

At the present stage, the social economy in China has developed by leaps and bounds. China's pig industry has been well developed un-der the background of this era. However, the number of pig diseases increases due to the influence of many factors, and highly pathogenic porcine reproductive and respiratory syndrome(HP-PRRS) is one of the very common diseases, causing growing mortality rate of pigs. The disease increases the economic loss of pig industry to a large extent, and brings difficulties in prevention and control of pig diseases. The staff must analyze and sum-marize the basic characteristics of pig diseases, and combine some practical problems in prevention and control of pig disease to find effective mea-sures. The scope and scale of pig production in different parts of China have been continuously expanded under the background of further develop-ment of pig industry in the new era. But for pig production, pig disease has been a problem that delays its further development. Related personnel must analyze and summarize the status quo of HP-PRRS, to find effective prevention measures and strategy.

Identification and Treatment of Swine Influenza and Common Cold in Veterinary Clinic

[Objective] The paper was to investigate the differentiation and treatment of swine influenza and common cold in veterinary clinic.[Method] The clinical data of diseased pigs from May 2017 to May 2018 were analyzed retrospectively to explore the clinical treatment methods for swine influenza and common cold, and the effectiveness and accuracy of clinical diagnosis was further improved. [Result] Through in-depth analysis of test results, we obtained a comprehensive understanding of the main causes of swine influenza and common cold, and found that the causes in-cluded improper breeding management of pigs, nutrition of pigs and lack of immunity of newborn pigs. [Conclusion] Analysis of the causes of swine influenza and common cold and suggestion of targeted clinical treatment measures will reduce the incidence of swine disease, and promote the vet-erinary clinical treatment effect of swine influenza and common cold.

基于转录组测序挖掘犊牦牛肾周脂质沉积关键基因

【目的】比较不同日龄犊牦牛肾周脂肪组织转录组表达谱的变化,挖掘调控犊牦牛肾周脂质沉积的信号通路和关键基因。【方法】选取1、7和30日龄各3头健康雄性犊牦牛,放血处死,采集肾周脂肪组织,利用Illumina HiSeq平台进行转录组测序,对转录组数据进行质控、比对、差异表达基因(DEGs)筛选,并对DEGs进行GO功能和KEGG通路富集分析。随机选取7个基因进行实时荧光定量PCR验证。【结果】转录组结果显示,在1和7日龄比较组中共鉴定到1627个显著DEGs,其中939个上调,688个下调;在7和30日龄比较组中共鉴定到3979个显著DEGs,其中1925个上调,2054个下调。GO功能和KEGG通路富集分析结果显示,在1和7日龄比较组中DEGs富集到酰基辅酶A脱氢酶活性条目,参与了脂肪酸生物合成、胰岛素抵抗、PPAR信号通路及鞘磷脂信号通路等;在7和30日龄比较组中DEGs富集到脂肪酸代谢、脂质代谢、磷酸水解酶活性及脂肪酶活性等多个生物过程,参与了丁酸盐代谢、脂肪酸代谢、甘油磷脂代谢、磷脂酶D信号通路及AMPK信号通路等。从富集通路中发现,SCD、ADIPOQ、PLIN1、ACADM和LPL等基因是与犊牦牛肾周脂质沉积相关的候选基因。实时荧光定量结果显示,7个基因的表达与转录组测序结果一致。【结论】本研究筛选出SCD、ADIPOQ、PLIN1、ACADM和LPL共5个与脂肪细胞分化和脂质沉积相关的基因。研究结果为进一步探究不同生长阶段犊牦牛肾周脂质沉积提供理论依据。