Objective:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus fiiretianus by using rat model of atherosclerosis.Methods:The air-flriefl powdered Mallotus fiiretianus leave...Objective:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus fiiretianus by using rat model of atherosclerosis.Methods:The air-flriefl powdered Mallotus fiiretianus leaves were extracted with ethanol and then evaporated.The ethanol extract was experienced Diaion HP-20 CC with a gradient of MeOH and H<sub>2</sub>O（50:50,100:0,v/v） and two fractions,Mallotus fiiretianus A（Mf A） and Mallotus fiiretianus B（MfB） were obtained.RaLs were divided into control,atherosclerosis and vitamin E,Mf A and Mf B treated groups.Atherosclerotic model was established by administering u loading dose of vitamin D<sub>3</sub> and feeding standard diet enriched with 2%cholesterol,0.5%porcine chelate,0.2%methimazole,5%sugar,10%pork fat. Vitamin E（0.20 g/kg）,Mf A（0.053 g/kg）,Mf B（0.057 g/kg）（with the potential） were administered to interfere with the development of atherosclerosis.After 9 weeks,rats were sacrificed and the blood lipid as well as composition of bile was examined.In addition,the thoracic aorta was harvested to evaluate histological changes and the intima-media thickness ratio.Results: Atherosclerosis model was successfully established,administration of vitamin E,Mf A and Mf B increased excretion of total bilirubin in bile,decreased triglycridc（TO）,total cholesterol（TC）, low density lipoprotein-cholesterol（LDL-C） level,enhanced ratio of high density lipoprotein-cholesterol and LDL-C in blood,improved histological changes and diminished intimamedia thickness ratio of thoracic aorta in atherosclerotic rats.As for the difference in antiatherosclerotic effects between Mf A and MfB,Mf A may be more powerful in declining TG level and Mf B may be more effective in decreasing TC level.Conclusions:The two different extracts. Mf A and Mf B can prevent the development of atherosclerosis.In detail,Mf A is more effective in regulating TG level and Mf B is more powerful in modulating TC.level in atherosclerotic rats.展开更多
The repairment after peripheral nerve injury is a very important field in medical research.Exosomes(Exo)is a natural nano-scale carrier for drugs.They can mediate the transfection of RNA and other signal molecules int...The repairment after peripheral nerve injury is a very important field in medical research.Exosomes(Exo)is a natural nano-scale carrier for drugs.They can mediate the transfection of RNA and other signal molecules into cells.Therefore,Exo can be used to bring the genetic materials into target cells,and regulate the expression of related genes.In this study,microRNA-21-5p(miR-21-5p)was introduced into BMSCs-derived Exo.After transfecting into DRG neurons,we uncovered their effects on stimulating the growth of DRG neurons and explored possible mechanis.The exosomes engineered with miR-21-5p/miR-NC were prepared by electroporation to obtain iExosomes-21/iExosomes-NC.DRG neurons of SD rats were cultured in vitro.展开更多
基金supported by Piaopu grant from Hainan Medical College,China(No.200309)
文摘Objective:To compare the anti-atherosclerotic effects of two different extracts from the leaves of Mallotus fiiretianus by using rat model of atherosclerosis.Methods:The air-flriefl powdered Mallotus fiiretianus leaves were extracted with ethanol and then evaporated.The ethanol extract was experienced Diaion HP-20 CC with a gradient of MeOH and H<sub>2</sub>O（50:50,100:0,v/v） and two fractions,Mallotus fiiretianus A（Mf A） and Mallotus fiiretianus B（MfB） were obtained.RaLs were divided into control,atherosclerosis and vitamin E,Mf A and Mf B treated groups.Atherosclerotic model was established by administering u loading dose of vitamin D<sub>3</sub> and feeding standard diet enriched with 2%cholesterol,0.5%porcine chelate,0.2%methimazole,5%sugar,10%pork fat. Vitamin E（0.20 g/kg）,Mf A（0.053 g/kg）,Mf B（0.057 g/kg）（with the potential） were administered to interfere with the development of atherosclerosis.After 9 weeks,rats were sacrificed and the blood lipid as well as composition of bile was examined.In addition,the thoracic aorta was harvested to evaluate histological changes and the intima-media thickness ratio.Results: Atherosclerosis model was successfully established,administration of vitamin E,Mf A and Mf B increased excretion of total bilirubin in bile,decreased triglycridc（TO）,total cholesterol（TC）, low density lipoprotein-cholesterol（LDL-C） level,enhanced ratio of high density lipoprotein-cholesterol and LDL-C in blood,improved histological changes and diminished intimamedia thickness ratio of thoracic aorta in atherosclerotic rats.As for the difference in antiatherosclerotic effects between Mf A and MfB,Mf A may be more powerful in declining TG level and Mf B may be more effective in decreasing TC level.Conclusions:The two different extracts. Mf A and Mf B can prevent the development of atherosclerosis.In detail,Mf A is more effective in regulating TG level and Mf B is more powerful in modulating TC.level in atherosclerotic rats.
文摘The repairment after peripheral nerve injury is a very important field in medical research.Exosomes(Exo)is a natural nano-scale carrier for drugs.They can mediate the transfection of RNA and other signal molecules into cells.Therefore,Exo can be used to bring the genetic materials into target cells,and regulate the expression of related genes.In this study,microRNA-21-5p(miR-21-5p)was introduced into BMSCs-derived Exo.After transfecting into DRG neurons,we uncovered their effects on stimulating the growth of DRG neurons and explored possible mechanis.The exosomes engineered with miR-21-5p/miR-NC were prepared by electroporation to obtain iExosomes-21/iExosomes-NC.DRG neurons of SD rats were cultured in vitro.
