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Protein Profile of Human Lung Squamous Carcinoma Cell Line NCI-H226 被引量:2
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作者 HAO ZHANG NA LI +5 位作者 YUE CHEN LING-YUN HUANG yi-ching wang GANG FANG DA-CHENG HE XUE-YUAN XIAO 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2007年第1期24-32,共9页
Objective To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer. Method Proteomic technique was used to analyze human lung squ... Objective To construct a database of human lung squamous carcinoma cell line NCI-H226 and to facilitate discovery of novel subtypes markers of lung cancer. Method Proteomic technique was used to analyze human lung squamous carcinoma cell line NCI-H226. The proteins of the NCI-H226 cells were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Results The results showed that a good reproducibility of the 2-D gel pattern was attained. The position deviation of matched spots among three 2-D gels was 1.95±0.53 mm in the isoelectric focusing direction, and 1.73±0.45 mm in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis direction. One hundred and twenty-seven proteins, including enzymes, signal transduction proteins, structure proteins, transport proteins, etc. were characterized, of which, 29 identified proteins in NCI-H226 cells were reported for the first time to be involved in lung cancer carcinogenesis. Conclusion The information obtained from this study could provide some valuable clues for further study on the carcinogenetic mechanism of different types of lung cancer, and may help us to discover some potential subtype-specific biomarkers of lung cancer. 展开更多
关键词 Lung squamous carcinoma NCI-H226 cell line PROTEOMICS Two-dimensional datapase
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马来西亚航空公司MH370次航班:客机坠海分析
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作者 Goong Chen Cong Gu +6 位作者 Philip J. Morris Eric G. Paterson Alexey Sergeev yi-ching wang Tomasz Wierzbicki 叶其孝 吴庆宝 《数学译林》 2016年第1期1-16,共16页
2014年3月8日马来西亚航空公司从吉隆坡至北京的MH370航班起飞后一个小时就失联了.波音777—200ER飞机共有12名机组人员和227名乘客.3月24日,马来西亚总理宣布,“因此,怀着深深的悲哀和遗憾,我必须告诉你们…MH370航班的飞行在南... 2014年3月8日马来西亚航空公司从吉隆坡至北京的MH370航班起飞后一个小时就失联了.波音777—200ER飞机共有12名机组人员和227名乘客.3月24日,马来西亚总理宣布,“因此,怀着深深的悲哀和遗憾,我必须告诉你们…MH370航班的飞行在南印度洋终结了.”虽然MH370航班的确切下落仍然不明,但是现有的证据表明,飞机坠入了海洋.然而,令人不安的是因为并非被称为“迫降海上”的所有的应急水上降落,当他们得到控制时都会以悲剧告终.在2009年1月15日“哈德森河上的迫降奇迹”中,机长ChelseyB.“Sully”Sullenberger。)及其机组人员把从拉瓜迪亚机场起飞,由于飞鸟击中飞机而导致断电后的美国航空公司1549航班的飞机——空客A320—200——成功地迫降在哈得逊河上.没有造成人员伤亡. 展开更多
关键词 航空公司 马来西亚 航班 客机 机组人员 波音777 南印度洋 人员伤亡
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