Microcystic/reticular schwannoma is a recently described variant of schwannoma with a predilection for the gastrointestinal tract. Due to overlapping features with other tumors, unawareness of this tumor type may lead...Microcystic/reticular schwannoma is a recently described variant of schwannoma with a predilection for the gastrointestinal tract. Due to overlapping features with other tumors, unawareness of this tumor type may lead to diagnostic and therapeutic pitfalls. We here report a case of microcystic/reticular schwannoma arising in the meso-appendix of a 43-year-old woman. The tumor was incidentally discovered by computed tomography scan for unrelated reasons. A laparoscopic operation was performed shortly after admission. Histological examination revealed a circumscribed tumor with a striking microcystic and cribriform architecture. Immunohistochemically, the tumor cells were diffusely positive for S100 protein, glial fibrillary acid protein and protein gene product 9.5, which were consistent with a peripheral nerve sheath tumor. The patient remains well with no signs of recurrence at a 10-mo follow-up. To our knowledge, this is the first case of microcystic/reticular schwannoma arising in the meso-appendix. Albeit very rare, microcystic/reticular schwannoma should be included in the differential diagnosis of appendiceal tumors.展开更多
Objective:To analyze clinicopathologic classification features of the cases that were diagnosed as missed abortion preoperatively but turn out to be hydatidiform mole(HM)postoperatively.Methods:A retrospective analysi...Objective:To analyze clinicopathologic classification features of the cases that were diagnosed as missed abortion preoperatively but turn out to be hydatidiform mole(HM)postoperatively.Methods:A retrospective analysis was conducted on the patients who had a missed abortion in our hospital from 2017 to 2018.Clinical and pathological characteristics of different types of HMs were observed.Diagnostic value of karyotype in HM was discussed based on the karyotype analysis of villi chromosome.Results:A total of 278(11.2%)HM patients were misdiagnosed as missed abortion.Naked-eye detection rate of HM was 26.61%,and sensitivity of transvaginal ultrasound on HM was 7.91%.One hundred and forty-seven(52.88%)HM cases could not be genotyped via pathological hematoxylin and eosin(HE)staining.70 HM patients underwent P57 immunohistochemistry,which had guiding significance to the classification.In addition,the karyotype diagnosis of the tissues from 15 cases basically matched their P57 classifications.Conclusions:P57 should be listed as a routine test in hydatidiform pathological examination at the same time of HE staining,and what’s more,P57 expression is consistent with genotyping,which should be recommended for the patients with HM if observed by naked eye.展开更多
Mangroves comprise a globally significant intertidal ecosystem that contains a high diversity of microorganisms,including fungi,bacteria and archaea.Archaea is a major domain of life that plays important roles in biog...Mangroves comprise a globally significant intertidal ecosystem that contains a high diversity of microorganisms,including fungi,bacteria and archaea.Archaea is a major domain of life that plays important roles in biogeochemical cycles in these ecosystems.In this review,the potential roles of archaea in mangroves are briefly highlighted.Then,the diversity and metabolism of archaeal community of mangrove ecosystems across the world are summarized and Bathyarchaeota,Euryarchaeota,Thaumarchaeota,Woesearchaeota,and Lokiarchaeota are confirmed as the most abundant and ubiquitous archaeal groups.The metabolic potential of these archaeal groups indicates their important ecological function in carbon,nitrogen and sulfur cycling.Finally,some cultivation strategies that could be applied to uncultivated archaeal lineages from mangrove wetlands are suggested,including refinements to traditional cultivation methods based on genomic and transcriptomic information,and numerous innovative cultivation techniques such as single-cell isolation and high-throughput culturing(HTC).These cultivation strategies provide more opportunities to obtain previously uncultured archaea.展开更多
基金Supported by National Key Clinical Specialty Project and Key Clinical Program of the Chinese Ministry of Health and the National Natural Science Foundation of China,No.81272630
文摘Microcystic/reticular schwannoma is a recently described variant of schwannoma with a predilection for the gastrointestinal tract. Due to overlapping features with other tumors, unawareness of this tumor type may lead to diagnostic and therapeutic pitfalls. We here report a case of microcystic/reticular schwannoma arising in the meso-appendix of a 43-year-old woman. The tumor was incidentally discovered by computed tomography scan for unrelated reasons. A laparoscopic operation was performed shortly after admission. Histological examination revealed a circumscribed tumor with a striking microcystic and cribriform architecture. Immunohistochemically, the tumor cells were diffusely positive for S100 protein, glial fibrillary acid protein and protein gene product 9.5, which were consistent with a peripheral nerve sheath tumor. The patient remains well with no signs of recurrence at a 10-mo follow-up. To our knowledge, this is the first case of microcystic/reticular schwannoma arising in the meso-appendix. Albeit very rare, microcystic/reticular schwannoma should be included in the differential diagnosis of appendiceal tumors.
文摘Objective:To analyze clinicopathologic classification features of the cases that were diagnosed as missed abortion preoperatively but turn out to be hydatidiform mole(HM)postoperatively.Methods:A retrospective analysis was conducted on the patients who had a missed abortion in our hospital from 2017 to 2018.Clinical and pathological characteristics of different types of HMs were observed.Diagnostic value of karyotype in HM was discussed based on the karyotype analysis of villi chromosome.Results:A total of 278(11.2%)HM patients were misdiagnosed as missed abortion.Naked-eye detection rate of HM was 26.61%,and sensitivity of transvaginal ultrasound on HM was 7.91%.One hundred and forty-seven(52.88%)HM cases could not be genotyped via pathological hematoxylin and eosin(HE)staining.70 HM patients underwent P57 immunohistochemistry,which had guiding significance to the classification.In addition,the karyotype diagnosis of the tissues from 15 cases basically matched their P57 classifications.Conclusions:P57 should be listed as a routine test in hydatidiform pathological examination at the same time of HE staining,and what’s more,P57 expression is consistent with genotyping,which should be recommended for the patients with HM if observed by naked eye.
基金This work was financially supported by the National Natural Science Foundation of China(grant no.91851105,31970105,31622002,and 42007217)the Shenzhen Science and Technology Program(grant no.JCYJ20170818091727570 and KQTD20180412181334790)+2 种基金the Key Project of Department of Education of Guangdong Province(grant no.2017KZDXM071)China Postdoctoral Science Foundation(no.2018M630977)the CAS Interdisciplinary Innovation Team(grant No.JCTD-2018-16).
文摘Mangroves comprise a globally significant intertidal ecosystem that contains a high diversity of microorganisms,including fungi,bacteria and archaea.Archaea is a major domain of life that plays important roles in biogeochemical cycles in these ecosystems.In this review,the potential roles of archaea in mangroves are briefly highlighted.Then,the diversity and metabolism of archaeal community of mangrove ecosystems across the world are summarized and Bathyarchaeota,Euryarchaeota,Thaumarchaeota,Woesearchaeota,and Lokiarchaeota are confirmed as the most abundant and ubiquitous archaeal groups.The metabolic potential of these archaeal groups indicates their important ecological function in carbon,nitrogen and sulfur cycling.Finally,some cultivation strategies that could be applied to uncultivated archaeal lineages from mangrove wetlands are suggested,including refinements to traditional cultivation methods based on genomic and transcriptomic information,and numerous innovative cultivation techniques such as single-cell isolation and high-throughput culturing(HTC).These cultivation strategies provide more opportunities to obtain previously uncultured archaea.