BACKGROUND Gallbladder perforation and gastrointestinal fistula are rare but serious complications of severe acute pancreatitis(SAP).However,neither spontaneous gallbladder perforation nor cholecysto-colonic fistula h...BACKGROUND Gallbladder perforation and gastrointestinal fistula are rare but serious complications of severe acute pancreatitis(SAP).However,neither spontaneous gallbladder perforation nor cholecysto-colonic fistula has been reported in acalculous acute pancreatitis patients.CASE SUMMARY A 31-year-old male presenting with epigastric pain was diagnosed with hypertriglyceridemia-related SAP.He suffered from multiorgan failure and was able to leave the intensive care unit on day 20.Three percutaneous drainage tubes were placed for profound exudation in the peripancreatic region and left paracolic sulcus.He developed spontaneous gallbladder perforation with symptoms of fever and right upper quadrant pain 1 mo after SAP onset and was stabilized by percutaneous drainage.Peripancreatic infection appeared 1 mo later and was treated with antibiotics but without satisfactory results.Then multiple colon fistulas,including a cholecysto-colonic fistula and a descending colon fistula,emerged 3 mo after the onset of SAP.Nephroscopy-assisted peripancreatic debridement and ileostomy were carried out immediately.The fistulas achieved spontaneous closure 7 mo later,and the patient recovered after cholecystectomy and ileostomy reduction.We presume that the causes of gallbladder perforation are poor bile drainage due to external pressure,pancreatic enzyme erosion,and ischemia.The possible causes of colon fistulas are pancreatic enzymes or infected necrosis erosion,ischemia,and iatrogenic injury.According to our experience,localized gallbladder perforation can be stabilized by percutaneous drainage.Pancreatic debridement and proximal colostomy followed by cholecystectomy are feasible and valid treatment options for cholecysto-colonic fistulas.CONCLUSION Gallbladder perforation and cholecysto-colonic fistula should be considered in acalculous SAP patients.展开更多
The microstructure,orientation and magnetostrictive properties of a Fe_(81)Ga_(19)poly crystal grown by Bridgman method were investigated.A big central single crystal surrounded by some small crystals was observed.Lan...The microstructure,orientation and magnetostrictive properties of a Fe_(81)Ga_(19)poly crystal grown by Bridgman method were investigated.A big central single crystal surrounded by some small crystals was observed.Lane X-ray back-reflection confirmed the<001>orientation of the central crystal and deviation from the<001>direction for the edge crystals.Subgrain boundaries were monitored in the crystal.It was confirmed that orientations of all the subgrains were very close to the<001>direction.Measurement of the magnetostriction in different crystals indicated the strong anisotropy of the magnetostrictive properties.This work opens the possibility of preparing large-sized single crystal of FeGa alloys by using the Bridgman method.展开更多
The intestinal health of chick embryos is vital for their life-long growth,and exogenous nutrition intervention may provide sufficient nutrition for embryonic development.In the present study,we investigated the effec...The intestinal health of chick embryos is vital for their life-long growth,and exogenous nutrition intervention may provide sufficient nutrition for embryonic development.In the present study,we investigated the effect of in ovo injection of L-methionine(L-Met)on the intestinal structure and barrier function of chick embryos.There were 4 groups of treatments:the control(CON)group injected with phosphate-buffered saline(PBS)and the other 3 groups injected with 5,10,and 20 mg L-Met/egg,respectively.The injection was performed on embryonic day 9(E9),and intestinal samples were collected on the day of hatching for analysis.The results showed that,compared with the CON group,the groups administered an in ovo injection of L-Met increased relative weights of the duodenum,jejunum,and ileum(P<0.05).Hematoxylin and eosin(H&E)staining showed that the groups injected with 5,10,and 20 mg L-Met significantly increased villus height and crypt depth(P<0.05).Moreover,in ovo injection of 10 mg L-Met also increased the transepithelial electrical resistance(TEER)of the jejunum(P<0.05).Injection with 10 and 20 mg L-Met increased the expression of the tight junction proteins(ZO-1 and claudin-1)and the fluorescence signal intensity of Ki67 and villin proteins(P<0.05).Further,the protein expression of phospho-Janus kinase 2(p-JAK2)and phospho-signal transducer and activator of transcription 3(p-STAT3)was significantly increased by 10 or 20 mg L-Met injection(P<0.05).In conclusion,the injection of L-Met,especially at a dose of 10 mg,showed beneficial effects on the intestinal integrity of chick embryos due to the activation of the JAK2/STAT3 signaling pathway.Our results may provide new insights for regulating the intestinal development of embryonic chicks and the rapid growth of chicks after hatching.展开更多
基金Supported by Beijing Science and Technology Program,No.Z181100001618013Peking Union Medical College Education Reform Program,No.2019zlgc0116.
文摘BACKGROUND Gallbladder perforation and gastrointestinal fistula are rare but serious complications of severe acute pancreatitis(SAP).However,neither spontaneous gallbladder perforation nor cholecysto-colonic fistula has been reported in acalculous acute pancreatitis patients.CASE SUMMARY A 31-year-old male presenting with epigastric pain was diagnosed with hypertriglyceridemia-related SAP.He suffered from multiorgan failure and was able to leave the intensive care unit on day 20.Three percutaneous drainage tubes were placed for profound exudation in the peripancreatic region and left paracolic sulcus.He developed spontaneous gallbladder perforation with symptoms of fever and right upper quadrant pain 1 mo after SAP onset and was stabilized by percutaneous drainage.Peripancreatic infection appeared 1 mo later and was treated with antibiotics but without satisfactory results.Then multiple colon fistulas,including a cholecysto-colonic fistula and a descending colon fistula,emerged 3 mo after the onset of SAP.Nephroscopy-assisted peripancreatic debridement and ileostomy were carried out immediately.The fistulas achieved spontaneous closure 7 mo later,and the patient recovered after cholecystectomy and ileostomy reduction.We presume that the causes of gallbladder perforation are poor bile drainage due to external pressure,pancreatic enzyme erosion,and ischemia.The possible causes of colon fistulas are pancreatic enzymes or infected necrosis erosion,ischemia,and iatrogenic injury.According to our experience,localized gallbladder perforation can be stabilized by percutaneous drainage.Pancreatic debridement and proximal colostomy followed by cholecystectomy are feasible and valid treatment options for cholecysto-colonic fistulas.CONCLUSION Gallbladder perforation and cholecysto-colonic fistula should be considered in acalculous SAP patients.
基金financially supported by the National Natural Science Foundations of China (Nos.51331001 and 51520105002)。
文摘The microstructure,orientation and magnetostrictive properties of a Fe_(81)Ga_(19)poly crystal grown by Bridgman method were investigated.A big central single crystal surrounded by some small crystals was observed.Lane X-ray back-reflection confirmed the<001>orientation of the central crystal and deviation from the<001>direction for the edge crystals.Subgrain boundaries were monitored in the crystal.It was confirmed that orientations of all the subgrains were very close to the<001>direction.Measurement of the magnetostriction in different crystals indicated the strong anisotropy of the magnetostrictive properties.This work opens the possibility of preparing large-sized single crystal of FeGa alloys by using the Bridgman method.
基金supported by the National Natural Science Foundation of China(31972585)the Technical System of Poultry Industry of Guangdong Province,China(2021KJ128)。
文摘The intestinal health of chick embryos is vital for their life-long growth,and exogenous nutrition intervention may provide sufficient nutrition for embryonic development.In the present study,we investigated the effect of in ovo injection of L-methionine(L-Met)on the intestinal structure and barrier function of chick embryos.There were 4 groups of treatments:the control(CON)group injected with phosphate-buffered saline(PBS)and the other 3 groups injected with 5,10,and 20 mg L-Met/egg,respectively.The injection was performed on embryonic day 9(E9),and intestinal samples were collected on the day of hatching for analysis.The results showed that,compared with the CON group,the groups administered an in ovo injection of L-Met increased relative weights of the duodenum,jejunum,and ileum(P<0.05).Hematoxylin and eosin(H&E)staining showed that the groups injected with 5,10,and 20 mg L-Met significantly increased villus height and crypt depth(P<0.05).Moreover,in ovo injection of 10 mg L-Met also increased the transepithelial electrical resistance(TEER)of the jejunum(P<0.05).Injection with 10 and 20 mg L-Met increased the expression of the tight junction proteins(ZO-1 and claudin-1)and the fluorescence signal intensity of Ki67 and villin proteins(P<0.05).Further,the protein expression of phospho-Janus kinase 2(p-JAK2)and phospho-signal transducer and activator of transcription 3(p-STAT3)was significantly increased by 10 or 20 mg L-Met injection(P<0.05).In conclusion,the injection of L-Met,especially at a dose of 10 mg,showed beneficial effects on the intestinal integrity of chick embryos due to the activation of the JAK2/STAT3 signaling pathway.Our results may provide new insights for regulating the intestinal development of embryonic chicks and the rapid growth of chicks after hatching.
