Gastric cancer(GC)is a common gastrointestinal tumor.Gastric precancerous lesions(GPL)are the last pathological stage before normal gastric mucosa transforms into GC.However,preventing the transformation from GPL to G...Gastric cancer(GC)is a common gastrointestinal tumor.Gastric precancerous lesions(GPL)are the last pathological stage before normal gastric mucosa transforms into GC.However,preventing the transformation from GPL to GC remains a challenge.Traditional Chinese medicine(TCM)has been used to treat gastric disease for millennia.A series of TCM formulas and active compounds have shown therapeutic effects in both GC and GPL.This article reviews recent progress on the herbal drugs and pharmacological mechanisms of TCM in preventing the transformation from GPL to GC,especially focusing on antiinflammatory,anti-angiogenesis,proliferation,and apoptosis.This review may provide a meaningful reference for the prevention of the transformation from GPL to GC using TCM.展开更多
OBJECTIVE Recent studies have demonstrated that the Nlrp3 inflammasome serve as a central role in the pathogenesis of cardiovascular diseases and endothelial dysfunction occurs in association with several cardiovascul...OBJECTIVE Recent studies have demonstrated that the Nlrp3 inflammasome serve as a central role in the pathogenesis of cardiovascular diseases and endothelial dysfunction occurs in association with several cardiovascular risk factors.Given the demonstrated anti-inflammatory effects of aspirin,the present study was designed to test whether aspirin diminish NLRP3 inflammasome activation and prevent endothelium injury and associated coronary artery damage during LPS.METHODS Mouse carotid arterial endothelial cells(CAECs)were cultured and treated with 0.1-3 mmol·L^(-1) of aspirin in response to LPS(2μg·mL^(-1))stimuli.After 24 h,the Nlrp3 inflammasome complexes consist of varied proteins were analyzed by WB.NO and T-AOC in the supernatant was detected by ELISA.Intracellular reactive oxygen species(ROS)generation for 24 h was observed by DCF fluorescence.The mice were treated with aspirin(12.5 mg·kg^(-1) per day,62.5 mg·kg^(-1) per day,125 mg·kg^(-1) per day)and dexamethasone(0.0182 mg·kg^(-1)per day)for 7 d.The level of IL^(-1)β,IL^(-1)8 protein was detected by ELISA.RESULTS Immunofluorescence results showed the colocalization of Nlrp3 with ASC or caspase 1decrease in a concentration-dependent manner.Meanwhile,the expression of Nlrp3 and caspase 1protein was decreased with the concentration of aspirin,but no changes the expression of ASC protein.Nlrp3 protein levels in CAECs were 0.33-0.8-fold and cle-caspase 1 protein levels in CAECs were 0.48-1-fold compared to those in LPS stimulation when treated with 0.1-3 mmol·L^(-1) aspirin for 24 h(P<0.01).Aspirin significantly antagonized the effect of LPS on NO(1.22-1.91-fold that of LPS stimulation,P<0.01)and T-AOC expression(1.02-1.90-fold that of LPS stimulation,P<0.01).As the different concentration of aspirin treated,the generation of ROS was 0.51-1.10-fold that of LPS stimulation(P<0.01).In vivo data shown the level of IL^(-1)β,IL^(-1)8 protein from serum are in concordance with the level of Nlrp3 inflammasome activation.CONCLUSION We conclude that aspirin has anti-inflammatory properties,protecting CAECs fromLPS-induced injury by inhibition of NLRP3 inflammasome activation through ROS pathway.展开更多
基金Supported by the National Natural Science Foundation of China,No.81904064Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences,No.CI2021A03804 and No.CI2021A05052Fundamental Research Funds for the Central Public Welfare Research Institutes,No.ZZ14-YQ-023,No.ZXKT21017,and No.ZXKT21024.
文摘Gastric cancer(GC)is a common gastrointestinal tumor.Gastric precancerous lesions(GPL)are the last pathological stage before normal gastric mucosa transforms into GC.However,preventing the transformation from GPL to GC remains a challenge.Traditional Chinese medicine(TCM)has been used to treat gastric disease for millennia.A series of TCM formulas and active compounds have shown therapeutic effects in both GC and GPL.This article reviews recent progress on the herbal drugs and pharmacological mechanisms of TCM in preventing the transformation from GPL to GC,especially focusing on antiinflammatory,anti-angiogenesis,proliferation,and apoptosis.This review may provide a meaningful reference for the prevention of the transformation from GPL to GC using TCM.
基金supported by National Natural Science Foundation of China(81603587,81603668)Science and Technology Development Plan of Guangdong Province(2017A020211016)Youth Medical Talent Fund of Guangzhou University of Chinese Medicine(QNYC20170105)
文摘OBJECTIVE Recent studies have demonstrated that the Nlrp3 inflammasome serve as a central role in the pathogenesis of cardiovascular diseases and endothelial dysfunction occurs in association with several cardiovascular risk factors.Given the demonstrated anti-inflammatory effects of aspirin,the present study was designed to test whether aspirin diminish NLRP3 inflammasome activation and prevent endothelium injury and associated coronary artery damage during LPS.METHODS Mouse carotid arterial endothelial cells(CAECs)were cultured and treated with 0.1-3 mmol·L^(-1) of aspirin in response to LPS(2μg·mL^(-1))stimuli.After 24 h,the Nlrp3 inflammasome complexes consist of varied proteins were analyzed by WB.NO and T-AOC in the supernatant was detected by ELISA.Intracellular reactive oxygen species(ROS)generation for 24 h was observed by DCF fluorescence.The mice were treated with aspirin(12.5 mg·kg^(-1) per day,62.5 mg·kg^(-1) per day,125 mg·kg^(-1) per day)and dexamethasone(0.0182 mg·kg^(-1)per day)for 7 d.The level of IL^(-1)β,IL^(-1)8 protein was detected by ELISA.RESULTS Immunofluorescence results showed the colocalization of Nlrp3 with ASC or caspase 1decrease in a concentration-dependent manner.Meanwhile,the expression of Nlrp3 and caspase 1protein was decreased with the concentration of aspirin,but no changes the expression of ASC protein.Nlrp3 protein levels in CAECs were 0.33-0.8-fold and cle-caspase 1 protein levels in CAECs were 0.48-1-fold compared to those in LPS stimulation when treated with 0.1-3 mmol·L^(-1) aspirin for 24 h(P<0.01).Aspirin significantly antagonized the effect of LPS on NO(1.22-1.91-fold that of LPS stimulation,P<0.01)and T-AOC expression(1.02-1.90-fold that of LPS stimulation,P<0.01).As the different concentration of aspirin treated,the generation of ROS was 0.51-1.10-fold that of LPS stimulation(P<0.01).In vivo data shown the level of IL^(-1)β,IL^(-1)8 protein from serum are in concordance with the level of Nlrp3 inflammasome activation.CONCLUSION We conclude that aspirin has anti-inflammatory properties,protecting CAECs fromLPS-induced injury by inhibition of NLRP3 inflammasome activation through ROS pathway.
