The control of astronomical cycles on lacustrine fine-grained event Sedimentation——A case study of the Chunshang sub-member of the upper Es_(4) in the Dongying Sag

Fine-grained lacustrine sedimentation controlled by astronomical cycles remains a research weakness in sedimentology studies,as most studies have concentrated on how astronomical cycles affect the normal lacustrine fine-grained sedimentation,but how they affect the lacustrine fine-grained event sedimen-tation has been rarely studied.Therefore,this work researched the characteristics of event sedimentation by systematically observing the cores from 30 cored wells in the Shahejie Formation of the Dongying Sag at a depth of over 1800 m,with more than 4000 thin sections being authenticated and over 1000 whole rocks being analyzed by X-ray diffraction(XRD).The research object was the Chunshang Sub-member of Upper Es_(4) in the Fanye 1 well,as it had the most comprehensive analysis data and underwent the most continuous coring.We divided astronomical cycles into different orders and made corresponding curves using the gamma-ray(GR)curve,spectral analysis,power spectrum estimation,and module extreme values,there were 6 long eccentricity periods,22 short eccentricity periods,65.5 obliquity cycles,and 110.5 precession cycles in this sub-member.On this basis,this study analyzed the control of astronomical cycles on the lacustrine fine-grained event sedimentation,and the research shows deposits were developed by slide-slump,turbidities,hyperpycnites,and tempestites in the Chunshang Sub-member of the Upper Es_(4),with higher long eccentricity,the monsoon climate contributes to the formation of storm currents,while with lower long eccentricity,the surface deposits are severely eroded by rivers and rainfalls,thus developing the slide-slump,turbidities,and hyperpycnites.The relationship between the lacustrine fine-grained event sedimentation and astronomical cycles was studied in this case study,which can promote research on fine-grained sedimentary rocks in genetic dynamics and boost the theoretical and disciplinary development in fine-grained sedimentology.

Synergistic anticancer effect of exogenous wild-type p53 gene combined with 5-FU in human colon cancer resistant to 5-FU in vivo

AIM To investigate the anticancer effect of a recombinant adenovirus-mediated p53(r Ad-p53) combined with 5-fluorouracil(5-FU) in human colon cancer resistant to 5-FU in vivo and the mechanism of r Ad-p53 in reversal of 5-FU resistance.METHODS nude mice bearing human colon cancer SW480/5-FU(5-FU resistant) were randomly assigned to four groups(n = 25 each): control group, 5-FU group, r Ad-p53 group, and r Ad-p53 + 5-FU group. At 24 h, 48 h, 72 h, 120 h and 168 h after treatment, 5 mice were randomly selected from each group and sacrificed using an overdose of anesthetics. The tumors were removed and the protein expressions of p53, protein kinase C(PKC), permeability-glycoprotein(P-gp) and multidrug resistance-associated protein 1(MRP1)(Western blot) and apoptosis(TUNEL) were determined.RESULTS The area ratios of tumor cell apoptosis were larger in the r Ad/p53 + 5-FU group than that in the control, 5-FU and r Ad/p53 groups(P < 0.05), and were larger in the r Ad/p53 group than that of the control group(P < 0.05) and the 5-FU group at more than 48 h(P < 0.05). The p53 expression was higher in the r Ad/p53 and the r Ad/p53 + 5-FU groups than that of the control and 5-FU groups(P < 0.05), and were higher in the r Ad/p53 + 5-FU group than that of the r Ad/p53 group(P < 0.05). Overexpression of PKC, P-gp and MRP1 was observed in the 5-FU and control groups. In the r Ad/p53 + 5-FU group, the expression of P-gp and MRP1 was lower that of the control and 5-FU groups(P < 0.05), and the expression of PKC was lower than that of the control, 5-FU and r Ad/p53 groups at more than 48 h(P < 0.05). In the r Ad/p53 group, the expression of P-gp and MRP1 was lower that of the control and 5-FU groups at more than 48 h(P < 0.05), and the expression of PKC was lower than that of the control and 5-FU groups at more than 120 h(P < 0.05).CONCLUSION5-FU combined with r Ad-p53 has a synergistic anticancer effect in SW480/5-FU(5-FU resistance), which contributes to reversal of 5-FU resistance.

X-ray fluorescence microtomography based on polycapillary- focused X-rays from laboratory source

X-ray fluorescence microtomography(μXFCT)is a nondestructive analytical technique and has been widely used to nondestructively detect and quantify the elemental composition and distributions in samples. Usually, synchrotron radiation X-rays are used for μXFCT, due to its high flux density. In this paper, a laboratory-sourcebased μXFCT system was developed, in which a polycapillary lens is employed to focus the X-ray beam and improve the flux density. The maximum likelihood expectation maximization algorithm was used to reconstruct the computed tomography slices at a limited number of projections. The experimental results demonstrated that the developed system could reveal the elemental distribution inside the test sample, with an elemental sensitivity of 1000 ppm.

Expression changes of hippocampal energy metabolism enzymes contribute to behavioural abnormalities during chronic morphine treatment

学习和记忆的依赖和缺陷是象 opioids 那样的滥用的药引起的二 well-establishedfeatures。马头鱼尾的怪兽是与两药依赖联系的一个重要区域并且学习并且记忆。然而，在到象 opioids 那样的滥用的药的马头鱼尾的怪兽追随者暴露的分子的事件很好没被理解。这里，我们由 proteomic 分析在海马趾的蛋白质表示上检验了长期的吗啡处理的效果。 Wefound 到为 10 天的吗啡的老鼠的那长期的暴露生产了柔韧的吗啡退却跳和记忆缺陷，并且也导致了三新陈代谢的酶的海马趾的蛋白质层次的一条重要down 规定包括 Fe-S 蛋白质 1 NADH 脱氢酶， pyruvate 建筑群和乳酸脱氢酶的 dihydrolipoamide acetyltransferase 或 E2 部件 2 。进一步即时的量的 PCR 分析证实相应 mRNAs 的层次显著地也被减少。与这些调查结果，更低的 ATP 层次和把葡萄糖变换成 ATP 的一个损害能力一致也在长期地对待的老鼠的马头鱼尾的怪兽被观察。显著地与吗啡附随地管理的 Opioid 对手 naltrexone 压制了吗啡退却跳并且颠倒了这些蛋白质的 down 规定。到吗啡的尖锐暴露也生产了跳的柔韧的吗啡退却和重要存储器缺陷，但是在吗啡管理显著地提高了 ATPlevels 并且压制了吗啡退却不在长期的对待吗啡的鼠标在尖锐 morphine-treatedbut 跳和存储器缺陷以前，没有通过 D 葡萄糖的这三 proteins.Intrahippocampal 注射的表达式到减少。D 葡萄糖的高剂量的 Intraperitoneal 注射在作为中央处理跳的导致吗啡的退却上显示出类似的效果。总起来说，我们的结果建议在由于长期的吗啡处理的马头鱼尾的怪兽的三新陈代谢的酶的那减少的表情贡献象吗啡退却跳和记忆缺陷那样的导致药的症状的发展。

Hard X-ray focusing resolution and efficiency test with a thickness correction multilayer Laue lens

The multilayer Laue lens(MLL) is a diffractive focusing optical element which can focus hard X-rays down to the nanometer scale. In this study, a WSi_(2)/Si multilayer structure consisting of 1736 layers, with a 7.2-nm-thick outermost layer and a total thickness of 17 μm, is prepared by DC magnetron sputtering. Regarding the thin film growth rate calibration, we correct the long-term growth rate drift from 2 to 0.6%, as measured by the grazing incidence X-ray reflectivity(GIXRR). A one-dimensional line focusing resolution of 64 nm was achieved,while the diffraction efficiency was 38% of the-1 order of the MLL Shanghai Synchrotron Radiation Facility(SSRF) with the BL15U beamline.

A 42-year-old woman with 4H leukodystrophy caused by a homozygous mutation in POLR3A gene

A 42-year-old woman,born to non-consanguineous parents with no family history of neurological diseases,suffered from ataxia for 3 years.After a normal psychomotor development,the patient first presented dental eruption at 1-year-old and with absence at 5 years of age.She stopped growing when she was 15 years old,and remained heights under 150 cm.Intellectual impairment became evident at 35 years old.Approximately 4 years ago (38 years old),she appeared secondary amenorrhea.Ataxia aggravated in the following 3 years and gradually affected daily life.On examinations,she has short stature [Supplementary Figure 1,http://links.lww.com/CM9/A61],tooth dysplasia and ataxia-related syndrome.There were no nystagmus,papilla atrophy or other visual problems.Her Mini-Mental State Examination (MMSE) score was 19,indicating mild intellectual disability.No contraceptives were used.She gave birth to a boy by making a cesarean section at 23-year-old,and her son was without any neurological symptom so far.Her parents died of other internal medicine diseases.Their gonads developed normally when they were alive.Only one brother who showed normal appearance and cognition was in her family.