酸性氯化物体系中电沉积AsSb合金的组成与结构

通过电沉积法在含As(III)和Sb(III)的盐酸溶液中制备AsSb合金(0.70%~95.81%As,质量分数),并研究电解质成分、盐酸浓度和温度对AsSb合金组成和结构的影响。通过热力学分析As(III)和Sb(III)在盐酸溶液中的电还原机理。结果表明,H+浓度的增加能促进As(III)的还原,而Cl^(−)浓度的增加则显著抑制Sb(III)的还原,因此,沉积物中的As含量随电解液中HCl浓度的上升而逐渐增加,同时,产物的结构从晶态转变为非晶态。当As含量为24.55%~33.75%(质量分数),产物为AsSb晶态与非晶态的混合物。电解温度对沉积物的组成影响较小,但AsSb合金的结构随温度的降低从晶态转变为非晶态。

Design of Omni-Directional Tilt Sensor Based on Machine Vision

This paper presents a new Omni-Directional Tilt Sensor (ODTS), which consists of the LED light, transparent cone-shaped closed container, mercury, camera, embedded systems and so on. The volume of the mercury in the container is equal to half of the container’s. When the detected surface is horizontal, the shape of mercury in the image captured by the camera is a black disc since the mercury is lightproof. When the detected surface tilts, the mercury flows and the mercury surface always maintains horizontally due to the gravity force of the earth. At this time, some area of the transparent cone-shaped closed container is not shaded by mercury and the border of the mercury’s shape in the captured image is a half circle and a half ellipse. Thus there is a translucent crescent-shaped area in the image. With analyzing this area by the specific algorithm based on machine vision, the tilt angle and directional angle can be obtained. The experimental results show that the ODTS proposed has some advantages, such as simple maintenance, high precision, wide range, low cost, real-time, reliability and high visualization.

Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis

Background:Circular RNA(circRNA)is a type of closed circular noncoding RNA(ncRNA),mostly formed by back-splicing or alternative splicing of pre-messenger RNA(mRNA).The aim of this study was to explore the expression profile of circRNA in peripheral blood mononuclear cells(PBMCs)of patients with ankylosing spondylitis(AS)and discover potential molecular markers of AS.Methods:The circRNA microarray technology was used to detect the expression of circRNAs in the peripheral blood of 6 patients with AS and 6 healthy controls(HC).To screen the differentially expressed circRNAs by fold change(FC)andP value,these differentially expressed circRNAs were analyzed by bioinformatics.In 60 cases of AS and 30 cases of HC,4 circRNAs were subjected to real-time fluorescence quantitative polymerase chain reaction(RT-qPCR),and their correlation with various clinical indicators was analyzed.Finally,the receiver operating characteristic(ROC)curve was used to analyze their potential as AS diagnostic markers.Results:The microarray results showed that there were 1369 significantly differently expressed(P<0.05,FC>1.5)circRNAs between the AS and HC groups(675 upregulated and 694 downregulated).The results of bioinformatics analysis suggested that they were mainly involved in"enzyme binding,""adenosine ribonucleotide binding,""MAPK signaling pathway",etc.The RT-qPCR results showed that the expressions of hsacircRNA001544(U=486.5,P<0.05)and hsacircRNA102532(U=645,P<0.05)were significantly different between the AS group and the HC group.The AS group was further divided into two subgroups:active AS(ASA)and stable AS(ASS).After analysis,it was found that compared with the HC group,hsacircRNA001544 was significantly increased in both ASA(U=214,P<0.05)and ASS groups(U=273,P<0.05),while hsacircRNA008961(U=250,P<0.05)and hsacircRNA102532(U=295,P<0.05)were only significantly increased in the ASA group.Furthermore,hsacircRNA012732 was significantly different between the ASA and ASS groups(U=194,P<0.05),and there was no statistical significance among the remaining groups.Correlation analysis results showed that hsacircRNA012732 was negatively correlated with Bath Ankylosing Spondylitis Disease Activity Index(BASDAI),high-sensitivity C-reactive protein(hsCRP),and globulin(GLOB)and positively correlated with lymphocyte count(LY),mean corpusular volume,and albumin(ALB),and hsacircRNA008961 was negatively correlated with platelet(PLT)count.ROC curve analysis showed that hsacircRNA001544(95%CI=0.610-0.831,P<0.05)and hsacircRNA102532(95%CI=0.521-0.762,P<0.05)were statistically significant,and their area under curve(AUC)values were 0.720 and 0.642,respectively.Conclusions:There are differentially expressed circRNAs in PBMCs of AS patients,and they may be involved in the occurrence and development of AS.Among these differentially expressed circRNAs,hsacircRNA012732 has the potential to become an indicator of disease activity,and hsacircRNA001544 has the potential to become a molecular marker for AS diagnosis.