Effect of Shengsui Jiangu capsule on TNF-α, TGF-β1, and empty bone lacuna in rats with alcoholic osteoporosis

TGF-Objective:To investigate the effect of postgraduate Shengsui Jiangu Capsule on tumor necrosis factors TNF-α,transforming growth factor TGF-β1 and empty bone lacuna in rats with alcoholic osteoporosis.Method:120 SD rats were randomly divided into 4 groups,30 rats in each group:blank group,model group,western medicine control group and traditional Chinese medicine intervention group.Liquor gavage was used to model the disease,except for the blank group.The western medicine control group was gavaged with a suspension of calcium carbonate and afad3,while the Chinese medicine intervention group was gavaged with a suspension of Shengsui Jiangu capsules combined with 0.9%saline solution injection.The values of TNF-αand TGF-β1 in femora were measured after 8,12,and 16 weeks of intervention.After dissecting out the femoral head using the HE(hematoxylin-eosin staining)staining method,the bone lacunae were observed under a light microscope,and the empty bone lacunae rate was calculated for intergroup comparison.Results:Compared with the blank group,the model group had a significantly higher percentage of TNF-αand empty bone lacunae,and a significantly lower percentage of TGF-β1(P<0.01).Compared with the model group,the rates of TNF-αand empty bone lacunae in the western medicine control group and the Chinese medicine intervention group were significantly decreased,and TGF-β1 was significantly increased(P<0.01).The traditional Chinese medicine intervention group was more effective than the western medicine control group(P<0.05).Conclusion:Shengsui Jiangu capsule attenuates the expression of TNF-α,enhances the expression of TGF-β1,decreases the rate of empty bone lacunae,and achieves a protective effect against alcoholic osteoporosis in rats with alcoholic osteoporosis.

在晶状体损伤诱导的视神经长时程再生过程中MMP-12的表达规律

目的:探讨晶状体损伤诱导SD大鼠视神经钳压损伤后轴突长时程再生过程中MMP-12的表达变化。方法:建立SD大鼠视神经损伤模型和晶状体损伤模型,将实验动物24只分为对照组(仅开放眼眶暴露视神经)、晶状体损伤组、视神经损伤组、晶状体损伤联合视神经损伤组,每组各6只大鼠。采用有参转录组测序分析损伤视神经区域差异基因表达变化,筛选相关高表达差异基因,通过实时荧光定量PCR(qRT-PCR)和酶联免疫吸附实验(ELISA)定量分析损伤区MMP-12的表达量变化。结果:转录组测序主成因分析表明,晶状体损伤联合视神经损伤是基因表达变化中的主要成因。基因表达差异分析显示,晶状体损伤联合视神经损伤组中,存在MMP-12基因表达上调。在建模成功后14、21d时,晶状体损伤联合视神经损伤组的MMP-12 mRNA表达量与对照组、视神经损伤组和晶状体损伤组比较上调(P<0.05);在7、28d时,各组间表达无差异。在建模成功后7、14、21d时,晶状体损伤联合视神经损伤组的MMP-12蛋白表达量与对照组和视神经损伤组比较上调(P<0.05);21d时,晶状体损伤联合视神经损伤组与视神经损伤组比较上调(P<0.05)。28d时,各组间表达无差异。结论:MMP-12表达上调可能参与晶状体损伤后诱导视神经长时程再生过程。