Previous studies have suggested that miR-324-3p is related to the pathophysiology of cerebral ischemia,but the mechanism underlying this relationship is unclea r.In this study,we found that miR-324-3p expression was d...Previous studies have suggested that miR-324-3p is related to the pathophysiology of cerebral ischemia,but the mechanism underlying this relationship is unclea r.In this study,we found that miR-324-3p expression was decreased in patients with acute ischemic stroke and in in vitro and in vivo models of ischemic stro ke.miR-324-3p agomir potentiated ischemic brain damage in rats subjected to middle cerebral artery occlusion,as indicated by increased infarct volumes and cell apoptosis rates and greater neurological deficits.In a PC12 cell oxygen-glucose deprivation/reoxygenation model,a miR-324-3 p mimic decreased cell viability and expression of the anti-apoptotic protein BCL2 and increased expression of the pro-apoptotic protein BAX and rates of cell apoptosis,whereas treatment with a miR-324-3p inhibitor had the opposite effects.Silencing miR-324-3p increased adenosine A1 receptor(A1R)expression thro ugh regulation of GATA binding protein 2(GATA2).These findings suggest that silencing miR-324-3p reduces ischemic brain damage via the GATA2/A1R axis.展开更多
Congenital chloride diarrhea(CCD;OMIM 214700)is an intestinal disease of an electrolyte defect characterized by dilated bowel loops,maternal polyhydramnios,premature birth,and lack of infant meconium[l].The incidence ...Congenital chloride diarrhea(CCD;OMIM 214700)is an intestinal disease of an electrolyte defect characterized by dilated bowel loops,maternal polyhydramnios,premature birth,and lack of infant meconium[l].The incidence of CCD is variable worldwide.Most cases are sporadic;however,there are three high-incidence areas,Finland,Poland,and Saudi Arabia.In addition,large genetic heterogeneity has been reported in about 300 sporadic patients with CCD from other ethnic groups[2-4].Undiagnosed or untreated CCD can be lethal within the first year of life,but with adequate treatment,patients are usually able to survive into adulthood[5,6].展开更多
基金funded by the National Natural Science Foundation of China,No.81803937(to YCM and QXD)Science and Technology Innovation Activity Plan for College Students of Zhejiang Province(Xinmiao Talent Plan),No.2020R413079(to AQZ)Wenzhou Science and Technology Plan Project,No.Y20210122(to QXD)。
文摘Previous studies have suggested that miR-324-3p is related to the pathophysiology of cerebral ischemia,but the mechanism underlying this relationship is unclea r.In this study,we found that miR-324-3p expression was decreased in patients with acute ischemic stroke and in in vitro and in vivo models of ischemic stro ke.miR-324-3p agomir potentiated ischemic brain damage in rats subjected to middle cerebral artery occlusion,as indicated by increased infarct volumes and cell apoptosis rates and greater neurological deficits.In a PC12 cell oxygen-glucose deprivation/reoxygenation model,a miR-324-3 p mimic decreased cell viability and expression of the anti-apoptotic protein BCL2 and increased expression of the pro-apoptotic protein BAX and rates of cell apoptosis,whereas treatment with a miR-324-3p inhibitor had the opposite effects.Silencing miR-324-3p increased adenosine A1 receptor(A1R)expression thro ugh regulation of GATA binding protein 2(GATA2).These findings suggest that silencing miR-324-3p reduces ischemic brain damage via the GATA2/A1R axis.
基金National Natural Science Foundation of China(No.81873594).
文摘Congenital chloride diarrhea(CCD;OMIM 214700)is an intestinal disease of an electrolyte defect characterized by dilated bowel loops,maternal polyhydramnios,premature birth,and lack of infant meconium[l].The incidence of CCD is variable worldwide.Most cases are sporadic;however,there are three high-incidence areas,Finland,Poland,and Saudi Arabia.In addition,large genetic heterogeneity has been reported in about 300 sporadic patients with CCD from other ethnic groups[2-4].Undiagnosed or untreated CCD can be lethal within the first year of life,but with adequate treatment,patients are usually able to survive into adulthood[5,6].
