Novel de novo TRPV4 mutation identified in a Chinese family with metatropic dysplasia inhibits chondrogenic differentiation

Metatropicdysplasia(MD,MIM156530)is a rare congenital bone dysplasia primarily characterized by severe platyspondyly with long and wide vertebral bodies and dumbbell deformity of the tubular bones.MD can be caused by heterozygous mutations in the gene encoding transient receptor potential vanilloid family member 4(TRPV4).We identifieda novel de novo mutation,c.2353T>C,in the TRPV4 gene,in a Chinese family with mild MD.

ScCas9 recognizes NNG protospacer adjacent motif in genome editing of rice

The CRISPR/Cas(clustered regularly in terspaced short palindromic repeats/CRISPR-associated protein)system has been widely used in genome editing,epigenetic modification,and other applications,because it is a precise,inexpensive,powerful and easy-to?use editing tool(Zhang et al.,2019).However,the scope of genome editing is limited by a short protospacer adjacent motif(PAM)specific sequence flanking the target site.Streptococcus pyogenes Cas9(5^Cas9),the most widely used Cas,strongly recognizes NGG PAM and partially recognizes NAG PAM in rice and is restricted to target the sites with these motifs(Anders et al.,2014;Meng et al.,2018).

Biomaterials for stem cell engineering and biomanufacturing

Recent years have witnessed the expansion of tissue failures and diseases.The uprising of regenerative medicine converges the sight onto stem cell-biomaterial based therapy.Tissue engineering and regenerative medicine proposes the strategy of constructing spatially,mechanically,chemically and biologically designed biomaterials for stem cells to grow and differentiate.Therefore,this paper summarized the basic properties of embryonic stem cells(ESCs),induced pluripotent stem cells(iPSCs)and adult stem cells.The properties of frequently used biomaterials were also described in terms of natural and synthetic origins.Particularly,the combination of stem cells and biomaterials for tissue repair applications was reviewed in terms of nervous,cardiovascular,pancreatic,hematopoietic and musculoskeletal system.Finally,stem-cell-related biomanufacturing was envisioned and the novel biofabrication technologies were discussed,enlightening a promising route for the future advancement of large-scale stem cell-biomaterial based therapeutic manufacturing.

Fast Carrier Selection of JPEG Steganography Appropriate for Application

In recent years,the improvement of the security of steganography mainly involves not only carrier security but also distortion function.In the actual environment,the existing method of carrier selection is limited by its complex algorithm and slow running speed,making it not appropriate for rapid communication.This study proposes a method for selecting carriers and improving the security of steganography.JPEG images are decompressed to spatial domain.Then correlation coefficients between two adjacent pixels in the horizontal,vertical,counter diagonal,and major diagonal directions are calculated.The mean value of the four correlation coefficients is used to evaluate the security of each JPEG image.The images with low correlation coefficients are considered safe carriers and used for embedding in our scheme.The experimental results indicate that the stego images generated from the selected carriers exhibit a higher anti-steganalysis capability than those generated from the randomly selected carriers.Under the premise of the same security level,the images with low correlation coefficients have a high capacity.Our algorithm has a very fast running speed,and the running time of a 2048×2048 image is less than 1 s.