The plant hormone ethylene regulates ripening in climacteric fruits.The phytohormone abscisic acid(ABA)affects ethylene biosynthesis,but whether ethylene influences ABA biosynthesis is unknown.To explore this possibil...The plant hormone ethylene regulates ripening in climacteric fruits.The phytohormone abscisic acid(ABA)affects ethylene biosynthesis,but whether ethylene influences ABA biosynthesis is unknown.To explore this possibility,we investigated the interactions between the ABA biosynthesis genes PpNCED2/3 and the ethylene response transcription factor PpERF3 in peach fruit.The ABA content increased during fruit maturation and reached a peak at stage S4 III.The increase was greatly inhibited by the ethylene inhibitor 1-MCP,which also suppressed PpERF3 expression.PpERF3 shared a similar expression profile with PpNCED2/3,encoding a rate-limiting enzyme involved in ABA biosynthesis,during fruit ripening.A yeast one-hybrid assay suggested that the nuclear-localized PpERF3 might bind to the promoters of PpNCED2/3.PpERF3 increased the expression of PpNCED2/3 as shown by dual-luciferase reporters,promoter-GUS assays and transient expression analyses in peach fruit.Collectively,these results suggest that ethylene promotes ABA biosynthesis through PpERF3’s regulation of the expression of ABA biosynthesis genes PpNCED2/3.展开更多
The toxic effects of 1-methyl-3-octylimidazolium bromide （[C8mim]Br） on wheat seedlings were evaluated. Wheat seedlings were cultivated in aqueous solution with [C8mim]Br at different concentrations （0, 1, 2, 4, 6,...The toxic effects of 1-methyl-3-octylimidazolium bromide （[C8mim]Br） on wheat seedlings were evaluated. Wheat seedlings were cultivated in aqueous solution with [C8mim]Br at different concentrations （0, 1, 2, 4, 6, 8 mg/L）. The contents of photosynthetic pigment and proline, peroxidation of membrane lipid, and activities of antioxidation enzymes （superoxide dismutase, catalase, peroxidase and ascorbate peroxidase） in leaves were measured on day 7 after treatment with [C8mim]Br. The results showed that [C8mim]Br significantly decreased the contents of photosynthetic pigments, activities of antioxidant enzymes in the wheat leaves and in dry weight of seedlings, while increased the proline content and membrane lipid peroxidation. The results suggested that [C8mim]Br can inhibit photosynthesis and lead to oxidative stress to wheat seedlings.展开更多
基金support of the National Natural Science Foundation of China[No.31501732]the Agricultural Science and Technology Innovation Program(ASTIP)[CAAS-ASTIP-2018-ZFRI].
文摘The plant hormone ethylene regulates ripening in climacteric fruits.The phytohormone abscisic acid(ABA)affects ethylene biosynthesis,but whether ethylene influences ABA biosynthesis is unknown.To explore this possibility,we investigated the interactions between the ABA biosynthesis genes PpNCED2/3 and the ethylene response transcription factor PpERF3 in peach fruit.The ABA content increased during fruit maturation and reached a peak at stage S4 III.The increase was greatly inhibited by the ethylene inhibitor 1-MCP,which also suppressed PpERF3 expression.PpERF3 shared a similar expression profile with PpNCED2/3,encoding a rate-limiting enzyme involved in ABA biosynthesis,during fruit ripening.A yeast one-hybrid assay suggested that the nuclear-localized PpERF3 might bind to the promoters of PpNCED2/3.PpERF3 increased the expression of PpNCED2/3 as shown by dual-luciferase reporters,promoter-GUS assays and transient expression analyses in peach fruit.Collectively,these results suggest that ethylene promotes ABA biosynthesis through PpERF3’s regulation of the expression of ABA biosynthesis genes PpNCED2/3.
基金supported by the National Natural Science Foundation of China(No. 20273019,20573034)the Innovation Project for University Prominent Research Talents of Henan,China(No. 2006KYCX021)the Project-sponsored by SRF for ROCS,SEM(No. [2006]331)
文摘The toxic effects of 1-methyl-3-octylimidazolium bromide （[C8mim]Br） on wheat seedlings were evaluated. Wheat seedlings were cultivated in aqueous solution with [C8mim]Br at different concentrations （0, 1, 2, 4, 6, 8 mg/L）. The contents of photosynthetic pigment and proline, peroxidation of membrane lipid, and activities of antioxidation enzymes （superoxide dismutase, catalase, peroxidase and ascorbate peroxidase） in leaves were measured on day 7 after treatment with [C8mim]Br. The results showed that [C8mim]Br significantly decreased the contents of photosynthetic pigments, activities of antioxidant enzymes in the wheat leaves and in dry weight of seedlings, while increased the proline content and membrane lipid peroxidation. The results suggested that [C8mim]Br can inhibit photosynthesis and lead to oxidative stress to wheat seedlings.
