This study has developed a novel sensing platform for the investigation of cell oxygen metabolism.With chitosan@TiO_(2) nanocomposites as supporting matrix which was decorated on the surface of indium tin oxide glass,...This study has developed a novel sensing platform for the investigation of cell oxygen metabolism.With chitosan@TiO_(2) nanocomposites as supporting matrix which was decorated on the surface of indium tin oxide glass,it can effectively absorb A549 cells and sensitize the electrochemiluminescence(ECL)of luminol.On this platform,the ECL output is dependent on the level of reactive oxygen species(ROSs),which was evidenced by the intervening of resveratrol,a typical ROSs’scavenger.The results indicated that the ECL signal was quenched by the resveratrol within its concentration range from 0.10 nM to 2.97μM.Thus,it is believable that the ECL of luminol sensitively responded upon oxygenic matters on this living cell platform would be powerful for cell oxygen metabolism monitoring.展开更多
基金the National Natural Science Foundation of China(21675115 and 21375091).
文摘This study has developed a novel sensing platform for the investigation of cell oxygen metabolism.With chitosan@TiO_(2) nanocomposites as supporting matrix which was decorated on the surface of indium tin oxide glass,it can effectively absorb A549 cells and sensitize the electrochemiluminescence(ECL)of luminol.On this platform,the ECL output is dependent on the level of reactive oxygen species(ROSs),which was evidenced by the intervening of resveratrol,a typical ROSs’scavenger.The results indicated that the ECL signal was quenched by the resveratrol within its concentration range from 0.10 nM to 2.97μM.Thus,it is believable that the ECL of luminol sensitively responded upon oxygenic matters on this living cell platform would be powerful for cell oxygen metabolism monitoring.
