Plasmids are useful tools for studying genetic information in living cells,as well as heterologous expression of genes and pathways in cells(Lauritsen et al.,2018).Various methods have been developed for plasmid manip...Plasmids are useful tools for studying genetic information in living cells,as well as heterologous expression of genes and pathways in cells(Lauritsen et al.,2018).Various methods have been developed for plasmid manipulation both in vivo and in vitro(Aslanidis and de Jong,1990;Li and Elledge,2007;Xia et al.,2018).However,large plasmids,such as P1-based artificial chromosomes(PACs),bacterial artificial chromosomes(BACs),and fosmids,are difficult to manipulate.展开更多
基金funded by the National Basic Research Program of China(973 Program)(2015CB554200)the National Natural Science Foundation of China(31870067,31670139,and 31800120)+1 种基金the CAMS Initiative for Innovative Medicine(2016-I2M-1-013)Fundamental Research Funds(2018RC310016)
文摘Plasmids are useful tools for studying genetic information in living cells,as well as heterologous expression of genes and pathways in cells(Lauritsen et al.,2018).Various methods have been developed for plasmid manipulation both in vivo and in vitro(Aslanidis and de Jong,1990;Li and Elledge,2007;Xia et al.,2018).However,large plasmids,such as P1-based artificial chromosomes(PACs),bacterial artificial chromosomes(BACs),and fosmids,are difficult to manipulate.
