For the design and optimization of functional peptides, unravelling the structures of individual building blocks as well as the properties of the ensemble is paramount. TI＇R1, derived from human transthyretin, is a f...For the design and optimization of functional peptides, unravelling the structures of individual building blocks as well as the properties of the ensemble is paramount. TI＇R1, derived from human transthyretin, is a fibril-forming peptide implicated in diseases such as familial amyloid polyneuropathy and senile systemic amyloidosis. The functional peptide TTR1-RGD, based on a TFR1 scaffold, was designed to specifically interact with cells. Here, we used scanning tunneling microscopy （STM） to analyze the assembly structures of TTRl-related peptides with both the reverse sequence and the modified forward sequence. The site- specific analyses show the following： i） The TIR1 peptide is involved in assembly, nearly covering the entire length within the ordered [3-sheet structures, ii） For TTR1-RGD peptide assemblies, the TTR1 motif forms the ordered [3-sheet while the RGDS motif adopts a flexible conformation allowing it to promote cell adhesion. The key site is clearly identified as the linker residue Gly13. iii） Close inspection of the forward and reverse peptide assemblies show that in spite of the difference in chemistry, they display similar assembling characteristics, illustrating the robust nature of these peptides, iv） Glycine linker residues are included in the ^-strands, which strongly suggests that the sequence could be optimized by adding more linker residues. These garnered insights into the assembled structures of these peptides help unravel the mechanism driving peptide assemblies and instruct the rational design and optimization of sequence- programmed peptide architectures.展开更多
Spinal cord injury （SCI） is an ever-increasing challenge. Severe injury can cause long-term loss of sensory and motor functions, as well as other chronic conditions, such as neuropathic pain and autonomic dysreflexi...Spinal cord injury （SCI） is an ever-increasing challenge. Severe injury can cause long-term loss of sensory and motor functions, as well as other chronic conditions, such as neuropathic pain and autonomic dysreflexia. So far, most research has been focused on acute injury. However, due to the lack of treatment, more and more individuals with this condition enter a chronic state, to which very little research effort has been dedicated.展开更多
Due to its openness and timeliness,the S&T Web information has become one of the most important resources for strategic intelligence monitoring.However,since S&T Web information is unstructured and lack of sem...Due to its openness and timeliness,the S&T Web information has become one of the most important resources for strategic intelligence monitoring.However,since S&T Web information is unstructured and lack of semantic description,it is a challenge to transfer the unstructured Web information into structured semantic knowledge.To solve this problem,the authors propose a method for structural monitoring of the S&T Web information resources.By using the knowledge extraction technologies,the authors firstly extract the knowledge objects as well as the relationship between objects from the Web resources and convert the free text into calculable structured knowledge units.Based on those extracted structured information,the authors build various kinds of monitoring models to realize research profiling for specific research fields.Based on those ideas,the authors implement the automated Web information monitoring system suitable for research field monitoring.A research profiling experiment also is carried out based on the semantic resources which are converted from the monitored Web data.展开更多
文摘For the design and optimization of functional peptides, unravelling the structures of individual building blocks as well as the properties of the ensemble is paramount. TI＇R1, derived from human transthyretin, is a fibril-forming peptide implicated in diseases such as familial amyloid polyneuropathy and senile systemic amyloidosis. The functional peptide TTR1-RGD, based on a TFR1 scaffold, was designed to specifically interact with cells. Here, we used scanning tunneling microscopy （STM） to analyze the assembly structures of TTRl-related peptides with both the reverse sequence and the modified forward sequence. The site- specific analyses show the following： i） The TIR1 peptide is involved in assembly, nearly covering the entire length within the ordered [3-sheet structures, ii） For TTR1-RGD peptide assemblies, the TTR1 motif forms the ordered [3-sheet while the RGDS motif adopts a flexible conformation allowing it to promote cell adhesion. The key site is clearly identified as the linker residue Gly13. iii） Close inspection of the forward and reverse peptide assemblies show that in spite of the difference in chemistry, they display similar assembling characteristics, illustrating the robust nature of these peptides, iv） Glycine linker residues are included in the ^-strands, which strongly suggests that the sequence could be optimized by adding more linker residues. These garnered insights into the assembled structures of these peptides help unravel the mechanism driving peptide assemblies and instruct the rational design and optimization of sequence- programmed peptide architectures.
文摘Spinal cord injury （SCI） is an ever-increasing challenge. Severe injury can cause long-term loss of sensory and motor functions, as well as other chronic conditions, such as neuropathic pain and autonomic dysreflexia. So far, most research has been focused on acute injury. However, due to the lack of treatment, more and more individuals with this condition enter a chronic state, to which very little research effort has been dedicated.
基金an outcome of the project "The computing method of subject centrality of texts based on language network"(No.61075047) supported by National Natural Science Foundation of China
文摘Due to its openness and timeliness,the S&T Web information has become one of the most important resources for strategic intelligence monitoring.However,since S&T Web information is unstructured and lack of semantic description,it is a challenge to transfer the unstructured Web information into structured semantic knowledge.To solve this problem,the authors propose a method for structural monitoring of the S&T Web information resources.By using the knowledge extraction technologies,the authors firstly extract the knowledge objects as well as the relationship between objects from the Web resources and convert the free text into calculable structured knowledge units.Based on those extracted structured information,the authors build various kinds of monitoring models to realize research profiling for specific research fields.Based on those ideas,the authors implement the automated Web information monitoring system suitable for research field monitoring.A research profiling experiment also is carried out based on the semantic resources which are converted from the monitored Web data.
