为研究不同草田轮作模式下土壤养分及细菌群落的组成特征,以5年紫花苜蓿-1年小麦(A5W1)、5年紫花苜蓿-1年玉米(A5C1)、5年紫花苜蓿-2年小麦(A5W2)和5年紫花苜蓿-2年玉米(A5C2)草田轮作模式为对象,测定了土壤有机质、全氮、全磷、全钾、...为研究不同草田轮作模式下土壤养分及细菌群落的组成特征,以5年紫花苜蓿-1年小麦(A5W1)、5年紫花苜蓿-1年玉米(A5C1)、5年紫花苜蓿-2年小麦(A5W2)和5年紫花苜蓿-2年玉米(A5C2)草田轮作模式为对象,测定了土壤有机质、全氮、全磷、全钾、碱解氮、速效磷和速效钾含量,在此基础上,基于16S rRNA基因序列扩增子测序研究了4种轮作模式下耕层土壤细菌群落组成特征。结果表明:轮作第2年各土壤养分含量较第1年显著下降。细菌组成研究结果显示,4种轮作模式下占优势的菌门为变形菌门、放线菌门和厚壁菌门。土壤细菌群落优势属以丙酸杆菌属(Propionibacterium)、芽孢杆菌属(Bacillus)、链球菌属(Streptococcus)以及奈瑟氏球菌属(Neisseria)为主。土壤细菌多样性以A5C2轮作处理最高,A5C1最低;聚类分析显示A5W1和A5C1的组成最接近,其次是A5C2,A5W2的组成与前两者相差较大。土壤养分含量与几种优势属之间呈显著相关性(P<0.05 or P<0.01)。研究结果揭示了河西走廊灌溉区种植紫花苜蓿多年后轮作小麦和玉米改善土壤肥力和防治某些土传植物病害微生物的机理。展开更多
In order to select the suitable alfalfa varieties for the shade areas in vineyard of Northwest, four shasing treatments Ro, R1 , R2, R3 （ full light, 60% of full light, 30% of full light, 10% of full light） were set...In order to select the suitable alfalfa varieties for the shade areas in vineyard of Northwest, four shasing treatments Ro, R1 , R2, R3 （ full light, 60% of full light, 30% of full light, 10% of full light） were set in the experiment to study the growth and physiological characteristics of seven domestic and foreign alfalfa varieties. The results showed that there was a big difference in height of alfalfa among cuhivars under shading; leaf area, leaf dry weight and leaf number reduced significantly with the decrease of light intensity （ P 〈 0.05 ） ; relative growth ratio, soluble sugar content, chlorophyll a/h decreased with the increase of shading in- tensity. The first harvest yield decreased significantly with the increasing shading intensity, AmeriStand and Queen declined less than others. In summary, the suit- able alfalfa cultivars for plantation were Queen, Alfalfa and AmeriStand in the northwest area where the habitat was similar with the habitat of experimental site, but due to the relativity of shading ability, the shading intensity could not exceed 50%.展开更多
文摘为研究不同草田轮作模式下土壤养分及细菌群落的组成特征,以5年紫花苜蓿-1年小麦(A5W1)、5年紫花苜蓿-1年玉米(A5C1)、5年紫花苜蓿-2年小麦(A5W2)和5年紫花苜蓿-2年玉米(A5C2)草田轮作模式为对象,测定了土壤有机质、全氮、全磷、全钾、碱解氮、速效磷和速效钾含量,在此基础上,基于16S rRNA基因序列扩增子测序研究了4种轮作模式下耕层土壤细菌群落组成特征。结果表明:轮作第2年各土壤养分含量较第1年显著下降。细菌组成研究结果显示,4种轮作模式下占优势的菌门为变形菌门、放线菌门和厚壁菌门。土壤细菌群落优势属以丙酸杆菌属(Propionibacterium)、芽孢杆菌属(Bacillus)、链球菌属(Streptococcus)以及奈瑟氏球菌属(Neisseria)为主。土壤细菌多样性以A5C2轮作处理最高,A5C1最低;聚类分析显示A5W1和A5C1的组成最接近,其次是A5C2,A5W2的组成与前两者相差较大。土壤养分含量与几种优势属之间呈显著相关性(P<0.05 or P<0.01)。研究结果揭示了河西走廊灌溉区种植紫花苜蓿多年后轮作小麦和玉米改善土壤肥力和防治某些土传植物病害微生物的机理。
基金Supported by GMKY 2014-12&GKG 1598006-3-12&GKG 15248003-21
文摘In order to select the suitable alfalfa varieties for the shade areas in vineyard of Northwest, four shasing treatments Ro, R1 , R2, R3 （ full light, 60% of full light, 30% of full light, 10% of full light） were set in the experiment to study the growth and physiological characteristics of seven domestic and foreign alfalfa varieties. The results showed that there was a big difference in height of alfalfa among cuhivars under shading; leaf area, leaf dry weight and leaf number reduced significantly with the decrease of light intensity （ P 〈 0.05 ） ; relative growth ratio, soluble sugar content, chlorophyll a/h decreased with the increase of shading in- tensity. The first harvest yield decreased significantly with the increasing shading intensity, AmeriStand and Queen declined less than others. In summary, the suit- able alfalfa cultivars for plantation were Queen, Alfalfa and AmeriStand in the northwest area where the habitat was similar with the habitat of experimental site, but due to the relativity of shading ability, the shading intensity could not exceed 50%.
