Effect of different drying methods on the taste and volatile compounds,sensory characteristics of Takifugu obscurus

Pufferfish is prone to deterioration due to abundant nutrients and high moisture content.Drying technology can extend the shelf life and enhance the flavor quality of aquatic products.The study investigated the effect of hot air drying(HAD),microwave vacuum drying(MVD)and hot air assisted radio frequency drying(HARFD)on the taste and volatile profiles of Takifugu obscurus.Different drying methods had significant influence on the color,rehydration,5’-nucleotides,free amino acids and volatile components(P<0.05).The results showed that HAD and HARFD could promote the flavor of T.obscurus by producing higher equivalent umami concentration(EUC)values,which were about two times of MVD group,and more pronounced pleasant odor according to sensory analysis.HAD is more appropriate for industrial application than HARFD and MVD considering the economic benefits.This study could provide a reference for the industrial application of drying T.obscurus.

Umami taste components in chicken-spices blends and potential effect of aroma on umami taste intensity

In this study,umami taste intensity(UTI)and umami taste components in chicken breast(CB)and chickenspices blends were characterized using sensory and instrumental analysis.Our main objective was to assess the aroma-umami taste interactions in different food matrices and reconcile the aroma-taste perception to assist future product development.The impact of key aroma,including vegetable-note"2-pentylfuran",meaty"methional",green"hexanal",and spicy-note-estragole and caryophyllene"on UTI was evaluated in monosodium glutamate and chicken extract.We found that spices significantly decreased UTI and umami taste components in CB.Interestingly,the perceptually similar odorants and tastants exhibited the potential to enhance UTI in food matrices.Methional was able to increase the UTI,whereas spicy and green-note components could reduce the UTI significantly.This information would be valuable to food engineers and formulators in aroma selection to control the UTI perceived by consumers,thus,improving the quality and acceptability of the chicken products.

Subdiaphragmatic abscess caused by the recently identifi ed pathogen Cutibacterium modestum: a case report

Subdiaphragmatic abscess is uncommon in patients who undergo resection for hepatocellular carcinoma.The identifi cation of pathogens and appropriate treatment can be challenging.[1]Herein,we present a case of subdiaphragmatic abscess caused by Cutibacterium modestum.A 66-year-old man was admitted to our hospital with fever,cough,and persistent,dull pain in the right upper quadrant for two days.The patient’s medical history included liver cirrhosis,radical resection of hepatocellular carcinoma three months prior to admission,and type 2 diabetes mellitus.Physical examination revealed tachycardia with small moist rales in the right lower chest.Abdominal examination revealed an L-shaped,well-healed surgical incision in the right upper quadrant without intra-abdominal mass or tenderness.

Endoscopic ultrasound-guided biliary drainage vs percutaneous transhepatic bile duct drainage in the management of malignant obstructive jaundice

BACKGROUND Malignant obstructive jaundice(MOJ)is a condition characterized by varying degrees of bile duct stenosis and obstruction,accompanied by the progressive development of malignant tumors,leading to high morbidity and mortality rates.Currently,the two most commonly employed methods for its management are percutaneous transhepatic bile duct drainage(PTBD)and endoscopic ultrasound-guided biliary drainage(EUS-BD).While both methods have demonstrated favorable outcomes,additional research needs to be performed to determine their relative efficacy.To compare the therapeutic effectiveness of EUS-BD and PTBD in treating MOJ.METHODS This retrospective analysis,conducted between September 2015 and April 2023 at The Third Affiliated Hospital of Soochow University(The First People’s Hospital of Changzhou),involved 68 patients with MOJ.The patients were divided into two groups on the basis of surgical procedure received:EUS-BD subgroup(n=33)and PTBD subgroup(n=35).Variables such as general data,preoperative and postoperative indices,blood routine,liver function indices,myocardial function indices,operative success rate,clinical effectiveness,and complication rate were analyzed and compared between the subgroups.RESULTS In the EUS-BD subgroup,hospital stay duration,bile drainage volume,effective catheter time,and clinical effect-iveness rate were superior to those in the PTBD subgroup,although the differences were not statistically significant(P>0.05).The puncture time for the EUS-BD subgroup was shorter than that for the PTBD subgroup(P<0.05).Postoperative blood routine,liver function index,and myocardial function index in the EUS-BD subgroup were significantly lower than those in the PTBD subgroup(P<0.05).Additionally,the complication rate in the EUS-BD subgroup was lower than in the PTBD subgroup(P<0.05).CONCLUSION EUS-BD may reduce the number of punctures,improve liver and myocardial functions,alleviate traumatic stress,and decrease complication rates in MOJ treatment.

血清CD62P、sTREM-1水平预测老年冠心病患者PCI术后主要心血管不良事件发生的价值

目的探究血清P选择素(CD62P)、可溶性髓样细胞表达触发因子受体1(sTREM-1)水平对老年冠心病患者经皮冠状动脉介入治疗(PCI)术后主要心血管不良事件发生的预测价值。方法选取2019年1月至2022年1月在本院就诊的160例老年冠心病患者为研究对象,采用流式细胞仪检测血清中CD62P水平;人sTREM-1 ELISA试剂盒检测血清中sTREM-1水平;根据出院后12个月的随访结果将老年冠心病患者分为未发生心血管不良事件组(108例)和发生心血管不良事件组(52例);多因素Logistic回归分析老年冠心病患者PCI术后主要心血管不良事件发生的危险因素;采用受试者工作特征(ROC)曲线评价血清CD62P、sTREM-1水平对老年冠心病患者PCI术后主要心血管不良事件发生的预测价值。结果与未发生心血管不良事件组比较,发生心血管不良事件组患者血清中CD62P、sTREM-1水平升高(P<0.05);老年冠心病患者糖尿病史、心绞痛史、冠脉病变个数、置入支架数目与发生心血管不良事件相关(P<0.05);多因素Logistic回归分析结果显示,CD62P高表达、sTREM-1高表达、有糖尿病史、有心绞痛史、冠脉病变个数>1、置入支架数目>2是老年冠心病患者PCI术后发生主要心血管不良事件的危险因素(P<0.05);CD62P、sTREM-1联合预测老年冠心病患者PCI术后发生主要心血管不良事件的AUC显著大于CD62P单独预测的AUC(Z=3.116,P=0.002)。结论血清CD62P、sTREM-1水平对老年冠心病患者PCI术后主要心血管不良事件发生可能具有一定的预测价值。

Exploration on Teaching Reform of Synthetic Biology Course Based on the New Engineering Concept

Synthetic biology is a new frontier of life science,which aims to design,transform and even synthesize organisms with engineering design concept.Doing a good job in the teaching of"synthetic biology"is of great significance to the cultivation and reserve of biotechnology professionals in China,and also has an important impact on students' employment competitiveness.Under the background of"new engineering",the course reform of"synthetic biology"was carried out in terms of the construction of teaching staff,teaching methods,students' participation and the innovation of course content,and specific reform suggestions were put forward,hoping to effectively promote the sustainable development of"synthetic biology"and effectively improve the quality of education.

Exploration of Teaching Reform of Food Machinery and Equipment Course Based on New Engineering Concept

The new engineering concept aims to train high-quality engineering talents to meet the needs of future science and technology and industrial development through the reform of education and teaching.Under the background of"new engineering",by introducing cutting-edge knowledge of the industry and interdisciplinary integration,adopting innovative teaching methods such as project-driven teaching and flipped classroom,strengthening experimental teaching and school-enterprise cooperation,and establishing comprehensive evaluation and feedback mechanism,Food Machinery and Equipment course is reformed to improve the teaching quality and train high-quality engineering talents to meet the needs of modern food processing industry.

Volatile profile and multivariant analysis of Sanhuang chicken breast in combination with Chinese 5-spice blend and garam masala

Sanhuang chicken is a popular native breed in China and well-known for delicious flavour.Spices could enhance the chicken meat flavour and work well in preservation.Chinese 5-spice blend(CS)and garam masala(GM)are routinely using spices in China and Pakistan,respectively.The flavour profiles of Sanhuang chicken breast(CB)and its blends with CS and GM were obtained by electronic nose(E-nose),solid-phase microextraction gas chromatography-mass spectrometry(SPME GC-MS)and GC-ion mobility spectrometry(GC-IMS).Principal component analysis(PCA)efficiently discriminated the aroma profiles of three chicken formulations.The GC-chromatographs revealed the significant aroma alterations of chicken breast meat after marination with spices.Aldehydes were the major contributors of chicken aroma,while most of the aromatic hydrocarbons were generated by spices.Almost all chicken key-compounds produced by oxidation reaction were either reduced or eliminated by marination,showing the antioxidation capacity of spices leading to meat preservation.GC-IMS is not only a rapid and comprehensive detection method,but also proved to be more sensitive than GC-MS.The substantial role of both traditional spices in enhancing flavour quality of chicken meat,and their exposure as functional ingredients in Chinese and Pakistan cuisines could lead to the cross-cultural meat trade opportunities.

Prolonging dual antiplatelet therapy improves the long-term prognosis in patients with diabetes mellitus undergoing complex percutaneous coronary intervention

OBJECTIVE To investigate the optimal duration of dual antiplatelet therapy(DAPT)in patients with diabetes mellitus(DM)requiring complex percutaneous coronary intervention(PCI).METHODS A total of 2403 patients with DM who underwent complex PCI from January to December 2013 were consecutively enrolled in this observational cohort study and divided according to DAPT duration into a standard group(11-13 months,n=689)and two prolonged groups(13-24 months,n=1133;>24 months,n=581).RESULTS Baseline characteristics,angiographic findings,and complexity of PCI were comparable regardless of DAPT duration.The incidence of major adverse cardiac and cerebrovascular event was lower when DAPT was 13-24 months than when it was 11-13 months or>24 months(4.6%vs.8.1%vs.6.0%,P=0.008),as was the incidence of all-cause death(1.9%vs.4.6%vs.2.2%,P=0.002)and cardiac death(1.0%vs.3.0%vs.1.2%,P=0.002).After adjustment for confounders,DAPT for 13-24 months was associated with a lower risk of major adverse cardiac and cerebrovascular event[hazard ratio(HR)=0.544,95%CI:0.373-0.795]and all-cause death(HR=0.605,95%CI:0.387-0.944).DAPT for>24 months was associated with a lower risk of all-cause death(HR=0.681,95%CI:0.493-0.942)and cardiac death(HR=0.620,95%CI:0.403-0.952).The risk of major bleeding was not increased by prolonging DAPT to 13-24 months(HR=1.356,95%CI:0.766-2.401)or>24 months(HR=0.967,95%CI:0.682-1.371).CONCLUSIONS For patients with DM undergoing complex PCI,prolonging DAPT might improve the long-term prognosis by reducing the risk of adverse ischemic events without increasing the bleeding risk.

Genome assembly of wild loquat (Eriobotrya japonica) and resequencing provide new insights into the genomic evolution and fruit domestication in loquat

Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding genes.Analysis of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its divergence.Genome resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic admixture.Compared with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic diversity.However,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication process.Large-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development stages.Unlike those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit development.These high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.

血管周细胞在表达PDGF-B的实体瘤中诱发造血

背景与目的肿瘤组织持续生长需要红细胞充分供氧。在各类病理生理情况下,成年哺乳动物可在主要造血器官骨髓之外发生髓外造血。然而,肿瘤组织能否造血是完全未知的。大量证据表明,在肿瘤微环境中,血管周细胞保留了祖细胞特性,可分化成其他细胞类型。在此,我们探究了肿瘤组织内周细胞是否能调节造血及其作用机制。方法使用鼠源周细胞进行全基因组表达谱分析以测试周细胞是否可向红细胞分化。使用NG2-CreERT2:R26R-tdTomato小鼠对血管周细胞进行在体遗传示踪。使用流式细胞术、单细胞测序和集落形成实验对分选细胞进行鉴定。使用定量聚合酶链反应(quantitative polymerase chain reactionq,PCR)、酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)、细胞磁珠分选和免疫染色检查红细胞生成素(erythropoietin,EPO)的表达。构建骨髓移植小鼠模型以研究骨髓对肿瘤红细胞生成的影响。结果表达谱显示,在PDGF-B处理后,NG2+周细胞表现出造血干祖样特征,并向红系谱系分化。同时,PDGF-B可靶向癌症相关成纤维细胞以产生促红细胞生成的关键激素EPO。通过遗传示踪及流式分析,我们定义了NG2+细胞衍生的造血亚群。进一步的单细胞测序和集落形成实验结果显示,在PDGF-B刺激下,肿瘤中的NG2+细胞形成了与典型的骨髓造血干细胞不同的红系祖细胞。结论本研究提出了肿瘤组织内造血的新概念,并阐明肿瘤周细胞分化为红细胞的分子机制。靶向肿瘤造血作为一种新的治疗理念,可能对肿瘤治疗产生深远影响。

A compact and closed-loop spin-exchange relaxation-free atomic magnetometer for wearable magnetoencephalography

Atomic magnetometers operated in the spin-exchange relaxation-free(SERF)regime are the promising sensor to replace superconducting quantum interference devices(SQUIDs)in the biomagnetism field.The SERF magnetometer with compact size and good performance is crucial to the new generation of wearable magnetoencephalography(MEG)system.In this paper,we developed a compact and closed-loop SERF magnetometer with the dimensions of 15.0×22.0×30.0 mm^(3)based on a single-beam configuration.The bandwidth of the magnetometer was extended to 675 Hz while the sensitivity was maintained at 22 f T/Hz^(1/2).A nearly 3-fold enhancement of the bandwidth was obtained in comparison with the open-loop control.The implementation of the closed-loop control also greatly improved the dynamic range,enabling the magnetometer to be robust against the disturbance of the ambient field.Moreover,the magnetometer was successfully applied for the detection of humanα-rhythm and auditory evoked fields(AEFs),which demonstrated the potential to be extended to multi-channel MEG measurements for future neuroscience studies.

Solar Active Region Magnetogram Generation by Attention Generative Adversarial Networks

Learning the mapping of magnetograms and EUV images is important for understanding the solar eruption mechanism and space weather forecasting.Previous works are mainly based on the pix2pix model for full-disk magnetograms generation and obtain good performance.However,in general,we are more concerned with the magnetic field distribution in the active regions where various solar storms such as the solar flare and coronal mass ejection happen.In this paper,we fuse the self-attention mechanism with the pix2pix model which allows more computation resource and greater weight for strong magnetic regions.In addition,the attention features are concatenated by the Residual Hadamard Production(RHP) with the abstracted features after the encoder.We named our model as RHP-attention pix2pix.From the experiments,we can find that the proposed model can generate magnetograms with finer strong magnetic structures,such as sunspots.In addition,the polarity distribution of generated magnetograms at strong magnetic regions is more consistent with observed ones.

Citrus β-carotene hydroxylase 2 (BCH2) participates in xanthophyll synthesis by catalyzing the hydroxylation of β-carotene and compensates for BCH1 in citrus carotenoid metabolism

Abstract As an essential horticultural crop,Citrus has carotenoid diversity,which affects its aesthetic and nutritional values.β,β-Xanthophylls are the primary carotenoids accumulated in citrus fruits,and non-heme di-iron carotene hydroxylase(BCH)enzymes are mainly responsible forβ,β-xanthophyll synthesis.Previous studies have focused on the hydroxylation of BCH1,but the role of its paralogous gene in citrus,BCH2,remains largely unknown.In this study,we revealed theβ-hydroxylation activity of citrus BCH2(CsBCH2)for the first time through the functional complementation assay using Escherichia coli,although CsBCH2 exhibited a lower activity in hydroxylatingβ-carotene intoβ-cryptoxanthin than citrus BCH1(CsBCH1).Our results showed that overexpression of CsBCH2 in citrus callus increased xanthophyll proportion and plastoglobule size with feedback regulation of carotenogenic gene expression.This study revealed the distinct expression patterns and functional characteristics of two paralogous genes,CsBCH1 and CsBCH2,and illustrated the backup compensatory role of CsBCH2 for CsBCH1 in citrus xanthophyll biosynthesis.The independent function of CsBCH2 and its cooperative function with CsBCH1 inβ-cryptoxanthin biosynthesis suggested the potential of CsBCH2 to be employed for expanding the synthetic biology toolkit in carotenoid engineering。

胸痛中心建设对急性ST段抬高型心肌梗死再灌注患者心功能及短期预后的影响

目的观察分析胸痛中心(chest pain center,CPC)建设对ST段抬高型心肌梗死(ST-segment elevation myocardial infarction,STEMI)患者再灌注治疗的效果及短期预后。方法选取CPC建设前后因急性胸痛就诊且诊断为STEMI行经皮冠状动脉介入治疗(percutaneous coronary intervention,PCI)治疗患者284例为研究对象,根据CPC建设时间分为CPC建设前的对照组136例和CPC建设后的观察组148例。分析2组一般资料、主要时间节点差异、治疗后心功能指标差异及术后住院期间心功能不良时间发生率。结果观察组发病至首次医疗接触时间(symptom-to-first medical contact,S2FMC)、首次医疗接触至首份心电图时间(first medical contact to first electrocardiogram,FMC2ECG)、入门至球囊扩张时间(door-to-ballon,D2B)及首次医疗接触至球囊扩张时间(first medical contact-to-balloon,FMC2B)均短于对照组(P<0.05),观察组术后左心室射血分数(left ventricular ejection fraction,LVEF)高于对照组,术后脑钠肽(brain natriuretic peptide,BNP)低于对照组(P<0.05),观察组急性心力衰竭、恶性心律失常、病死率均低于对照组(P<0.05)。结论CPC建设能够有效缩短STEMI患者发病至再灌注的时间,减少了心肌缺血时间,对患者术后心功能的恢复有显著效果,同时降低了术后短期心功能相关不良事件的发生,降低了STEMI患者的病死率,临床应用价值相对更高。

Thoughts on Cultivation of Top-notch Innovative Talents of Food Subject in Local Universities

The cultivation of top-notch innovative talents is one of the most prominent tasks in China s universities in the new era,and also an opportunity and challenge for local universities.Taking the food subject of local universities as an example,some suggestions were put forward from the aspects of security measures,teacher team construction,talent selection and management,and talent cultivation,in order to provide reference for the exploration and practice of cultivating top-notch innovative talents in the food subject of local universities.

猪肉嫩度测定方法对比分析

【目的】改进肌肉嫩度测定方法,达到简单、快速、易于操作,以期适用工业化生产。【方法】以45头杜长大三元杂交猪(DLY)背最长肌为素材,利用嫩度仪和质构仪,研究屠宰后8 h、24 h、48 h、72 h 4个时间点、不同肌纤维走向的冷却肉嫩度,以及72 h熟肉嫩度,对比分析不同时间点、肌纤维走向和不同仪器测定的肌肉剪切力值,建立冷却肉与熟肉间的数学模型。【结果】宰后8~72 h不同肌纤维走向(垂直、斜向)的冷却肉剪切力值变化趋势一致,二者间极显著正相关(p <0.01);宰后24 h冷却肉剪切力值与72 h熟肉剪切力值呈显著线性相关(p <0.05);国产嫩度仪与质构仪测定的肌肉剪切力值极显著正相关,相关系数达0.688 (p <0.01),而且二者间线性关系良好。【结论】生产中24 h排酸后可直接横切取背最长肌,钻取肉样进行剪切力值测定;屠后24 h冷却肉剪切力值可客观反映猪肉嫩度;国产嫩度仪可与质构仪互相替代,进行肌肉嫩度评定。

页岩气集输管网压力不均衡产生倒灌的对策

我国页岩气开发较晚,地面集输工艺技术还不成熟,目前国内大多非常规天然气田采用"枝上枝"管网形式布管,能在一定程度上减少建设和运营费用。在管网集输中常遇到页岩气低压单井倒灌现象,不但造成资源浪费,而且极易引发安全事故。针对这一现象,采取适时调整各单井集输压力、低压气井集输工艺,低压井口、集气阀组处设置橇装化增压装置以及单井设置止回阀等措施。随着页岩气田的发展,集气阀组会不断增多,所需橇装化增压装置也随之增加,优化管网形式和改进工艺技术才能从根本上解决管网倒灌问题。

Acupuncture for cerebral palsy: a meta-analysis of randomized controlled trials

OBJECTIVE： To evaluate the efficacy and safety of acupuncture therapy for children with cerebral palsy. DATA SOURCES： We conducted electronic searches of PUBMED（1950/2017）, EMBASE（1974/2017）, Science Direct（1986/2017）, Academic Source Premier（1887/2017）, the Cochrane Library（Issue 4, April 2017）, Science Citation Index Expanded（1900/2017）, China National Knowledge Infrastructure（1915/2017）, China Biological Medicine（1990/2017-04）, Wan Fang（1980/2017）, VIP（1989/2017）, and Chinese Science Citation Database（1989/2017）. DATA SELECTION： We included randomized controlled trials that aimed to compare the effect of acupuncture plus rehabilitation training versus rehabilitation training alone. Data about functional motor abilities, daily activity/social participation, effective rate, intellectual development, and adverse effects were included. We used Revman 5.2 software for statistical analysis. OUTCOME MEASURES： The primary outcomes included functional motor abilities, daily activity, and effective rate. The secondary outcomes included intellectual development and adverse effects.RESULTS： Twenty-one studies with a total of 1718 participants met the inclusion criteria. The effect size of gross motor function（SMD = 0.64, 95% CI： 0.52 to 0.76, P 〈 0.00001; I^2 = 0%, P = 0.69; in 13 studies with 1144 patients） and the total effective rate（RR = 1.28, 95% CI： 1.20 to 1.37, P 〈 0.00001; I^2 = 18%, P = 0.27; in 12 studies with 1106 patients） suggested that acupuncture plus rehabilitation produced a significant improvement in gross motor function and a high total effective rate. The pooled fine motor function（SMD = 3.48, 95% CI： 2.62 to 4.34, P 〈 0.00001; I^2 = 64%, P = 0.10; in 2 studies with 193 patients）, modified Ashworth scale scores（SMD = –0.31, 95% CI： –0.52 to –0.11, P = 0.003; I^2 = 74%, P = 0.004; in 5 studies with 363 patients） and activities of daily living（SMD = 1.45, 95% CI： 1.20 to 1.71, P 〈 0.00001; I^2 = 78%, P = 0.004; in 4 studies with 313 patients） also indicated improvements in children with cerebral palsy. Publication bias was not observed. Only mild adverse events related to acupuncture were reported. CONCLUSION： Acupuncture plus rehabilitation training improved gross motor function, reduced muscle spasms, and enhanced daily life activities in children with cerebral palsy. However, this conclusion should be interpreted with caution due to the small number of randomized controlled trials available and the small sample sizes. More high-quality and large-scale studies are needed.

Similarity-based denoising of point-sampled surfaces

A non-local denoising (NLD) algorithm for point-sampled surfaces (PSSs) is presented based on similarities, including geometry intensity and features of sample points. By using the trilateral filtering operator, the differential signal of each sample point is determined and called "geometry intensity". Based on covariance analysis, a regular grid of geometry intensity of a sample point is constructed, and the geometry-intensity similarity of two points is measured according to their grids. Based on mean shift clustering, the PSSs are clustered in terms of the local geometry-features similarity. The smoothed geometry intensity, i.e., offset distance, of the sample point is estimated according to the two similarities. Using the resulting intensity, the noise component from PSSs is finally removed by adjusting the position of each sample point along its own normal direction. Ex- perimental results demonstrate that the algorithm is robust and can produce a more accurate denoising result while having better feature preservation.