Objective Hepatic stellate cells(HSCs)play a crucial role in liver fibrosis.Early-stage liver fibrosis is reversible and intimately associated with the state of HSCs.Kruppel-like factor 4(KLF4)plays a pivotal role in ...Objective Hepatic stellate cells(HSCs)play a crucial role in liver fibrosis.Early-stage liver fibrosis is reversible and intimately associated with the state of HSCs.Kruppel-like factor 4(KLF4)plays a pivotal role in a wide array of physiological and pathological processes.This study aimed to investigate the effect of KLF4 on the proliferation,apoptosis and phenotype of quiescent HSCs Methods We designed a KLF4 lentiviral vector and a KLF4 siRNA lentiviral vector,to upregulate and silence KLF4 expression in human HSC LX-2 cells via transfection.Cell proliferation was assessed using the CCK-8 assay.Flow cytometry was used to detect the cell cycle distribution and apoptosis rate.Western blotting was used to determine the levels of some quiescence and activation markers of HSCs Results Overexpression of KLF4 significantly increased the levels of E-cadherin and ZO-1,which are quiescent HSC markers,while significantly decreased the levels of N-cadherin and a-SMA,known activated HSC markers.In contrast,cell proliferation and apoptosis rates were elevated in LX-2 cells in which KLF4 expression was silenced Conclusion KLF4 inhibits the proliferation and activation of human LX-2 HSCs.It might be a key regulatory protein in the maintenance of HSC quiescence and may serve as a target for the inhibition of hepatic fibrosis.展开更多
To the Editor：A choledochal cyst is an uncommon congenital anomaly characterized by single or multiple dilatations of the biliary tree.Traumatic rupture ofa choledochal cyst is an extremely rare event,with the first ...To the Editor：A choledochal cyst is an uncommon congenital anomaly characterized by single or multiple dilatations of the biliary tree.Traumatic rupture ofa choledochal cyst is an extremely rare event,with the first reported case in 1960 of a child who was kicked by a horse.[1] We report a case of choledochal cyst rupture due to blunt trauma in a Chinese woman.A 41-year-old Chinese woman without any previous medical history was admitted to the emergency department because of abdominal pain with 72 h of evolution.She had been assaulted by her husband,who kicked her several times in the upper abdomen.展开更多
基金supported by the National Natural Science Foundation of China(No.81071541).
文摘Objective Hepatic stellate cells(HSCs)play a crucial role in liver fibrosis.Early-stage liver fibrosis is reversible and intimately associated with the state of HSCs.Kruppel-like factor 4(KLF4)plays a pivotal role in a wide array of physiological and pathological processes.This study aimed to investigate the effect of KLF4 on the proliferation,apoptosis and phenotype of quiescent HSCs Methods We designed a KLF4 lentiviral vector and a KLF4 siRNA lentiviral vector,to upregulate and silence KLF4 expression in human HSC LX-2 cells via transfection.Cell proliferation was assessed using the CCK-8 assay.Flow cytometry was used to detect the cell cycle distribution and apoptosis rate.Western blotting was used to determine the levels of some quiescence and activation markers of HSCs Results Overexpression of KLF4 significantly increased the levels of E-cadherin and ZO-1,which are quiescent HSC markers,while significantly decreased the levels of N-cadherin and a-SMA,known activated HSC markers.In contrast,cell proliferation and apoptosis rates were elevated in LX-2 cells in which KLF4 expression was silenced Conclusion KLF4 inhibits the proliferation and activation of human LX-2 HSCs.It might be a key regulatory protein in the maintenance of HSC quiescence and may serve as a target for the inhibition of hepatic fibrosis.
文摘To the Editor：A choledochal cyst is an uncommon congenital anomaly characterized by single or multiple dilatations of the biliary tree.Traumatic rupture ofa choledochal cyst is an extremely rare event,with the first reported case in 1960 of a child who was kicked by a horse.[1] We report a case of choledochal cyst rupture due to blunt trauma in a Chinese woman.A 41-year-old Chinese woman without any previous medical history was admitted to the emergency department because of abdominal pain with 72 h of evolution.She had been assaulted by her husband,who kicked her several times in the upper abdomen.
