“三高三难”背景下职业院校虚拟仿真实训改革初探

高职教育的高质量发展,需要进行实训教学改革来解决当前实训存在的“高投入高难度高风险、难实施难观摩难再现”问题。虚拟仿真实训改革应结合教学实际、企业生产、区域发展,以现有实训基地建设为基础,以多种信息技术为手段,聚焦行业需求,搭建企业和学生双向需求通道,科学构建实训课程评价体系,整合校企课程资源,构建共建共享共用机制,实现大范围资源共享,最终实现高技能人才培养的信息化、智能化和现代化的目标。

Association between Serum Ferritin Levels and Metabolic-associated Fatty Liver Disease in Adults:a Cross-sectional Study Based on the NHANES

Objective Ferritin,initially acting as an iron-storage protein,was found to be associated with metabolic diseases.Our study was designed to investigate the association between serum ferritin and metabolic-associated fatty liver disease(MAFLD)using data from the National Health and Nutrition Examination Survey(NHANES)of the United State of America.Methods A cross-sectional study was conducted,enrolling a total of 2145 participants from the NHANES in the 2017–2018 cycles.Hepatic steatosis and liver fibrosis were assessed by ultrasound images and several non-invasive indexes.Multiple regression analysis was conducted to determine the associations between serum ferritin concentration and MAFLD and liver fibrosis.Results The analysis revealed that participants with higher serum ferritin levels(Q3 and Q4 groups)had a higher prevalence of MAFLD than those with the lowest serum ferritin levels[Q3 vs.Q1:OR=2.17(1.33,3.53),P<0.05 in fatty liver index(FLI);Q4 vs.Q1:OR=3.13(1.91,5.13),P<0.05 in FLI].Additionally,participants with the highest serum ferritin levels(Q4 group)displayed a higher prevalence of liver fibrosis[Q4 vs.Q1:OR=2.59(1.19,5.62),P<0.05 in liver stiffness measurement;OR=5.06(1.12,22.94),P<0.05 in fibrosis-4 index],with significantly increased risk observed in participants with concomitant diabetes[OR=7.45(1.55,35.72),P=0.012].Conclusion Our study revealed that elevated serum ferritin levels are associated with a higher prevalence of MAFLD and advanced liver fibrosis in patients.Elevated serum ferritin levels combined with diabetes are important risk factors for liver fibrosis.

DLAT as a Cuproptosis Promoter and a Molecular Target of Elesclomol in Hepatocellular Carcinoma

Objective Cuproptosis is a novel cell death pathway that was newly discovered in early 2022.However,cuproptosis is still in its infancy in many respects and warrants further research in hepatocellular carcinoma(HCC).This study aimed to analyze the mechanism of cuprptosis in HCC.Methods Herein,the tumor microenvironment infiltration landscape of molecular subtypes was illustrated using GSVA,ssGSEA,TIMER,CIBERSORT,and ESTIMATE algorithms based on the expression profile of cuproptosis-related genes(CRGs)from TCGA and GEO databases.Then,the least absolute shrinkage and selection operator regression method was applied to construct a cuproptosis signature to quantify the cuproptosis profile of HCC.Further,we explored the expression of three hub CRGs in cell lines and clinical patient tissues of HCC by Western blotting,qRT-PCR and immunohistochemistry.Finally,we examined the function of dihydrolipoamide S-acetyltransferase(DLAT)in cuproptosis in HCC by loss-of-function strategy,Western blotting and CCK8 assay.Results Three distinct molecular subtypes were identified.Cluster 2 had the greatest infiltration of immune cells with best prognosis.The cuproptosis signature was indicative of tumor subtype,immunity,and prognosis for HCC,and specifically,a low cuproptosis score foreshadowed good prognosis.DLAT was highly expressed in liver cancer cell lines and HCC tissues and positively correlated with clinical stage and grade.We also found that potent copper ionophore elesclomol could induce cuproptosis in a copper-dependent manner.Selective Cu^(++)chelator ammonium tetrathiomolybdate and downregulating DLAT expression by siRNA could effectively inhibit cuproptosis.Conclusion Cuproptosis and DLAT as a promising biomarker could help to determine the prognosis of HCC and may offer novel insights for effective treatment.

“双高计划”职业院校教辅人员绩效考核问题与对策

高职教育是我国高等教育的重要组成部分,高职院校管理水平的高低直接影响着学校的发展,而高职院校教辅人员群体的素质直接影响学校的管理水平,因此如何激发教辅人员的工作积极性是“双高”计划职业院校亟须解决的问题。新时代“双高计划”职业院校教辅人员绩效考核还存在一些问题,严重挫伤了教辅人员的主观能动性。“双高计划”职业院校教辅人员的绩效考核应以用好考核结果为导向,并引入现代化定量考核手段,同时完善绩效考核管理体系标准,注重有效面谈,以优化教辅人员绩效考核体系。

High Level of Ubiquitin Conjugate Enzyme E2O Indicates Poor Prognosis of Patients with Hepatocellular Carcinoma

Objective Ubiquitin conjugate enzyme E2O(UBE2O)is a ubiquitin-conjugating enzyme that has been reported to be involved in tumorigenesis.This study investigated the role of UBE2O in hepatocellular carcinoma(HCC).Methods The expression of UBE2O was detected using qRT-PCR,Western blotting,and immunohistochemical staining.Cell proliferation and Transwell assays were used to detect proliferation,migration,and invasion of HCC cells,respectively.Bioinformatic analysis was performed to analyze the relationship between UBE2O and the clinical features,prognosis,and immune cell infiltration of HCC.Results UBE2O was significantly over-expressed in HCC tissues.High expression of UBE2O was associated with poor tumor grade and poor prognosis.Functional experiments showed that down-regulation of UBE2O inhibited HCC cell proliferation,migration,and invasion.Co-expression gene analysis and gene set enrichment analysis showed that UBE2O was associated with protein hydrolysis,cell cycle,and cancer-related pathways in HCC.The results of immune analysis revealed that the expression of UBE2O was positively correlated with the immune infiltration and expression of immune-related chemokines of HCC.Conclusions UBE2O is significantly correlated with the prognosis of HCC and may be a valuable prognostic biomarker for HCC.

Effect of Saccharomyces cerevisiae on alfalfa nutrient degradation characteristics and rumen microbial populations of steers fed diets with different concentrate-to-forage ratios

Live yeast（Saccharomyces cerevisiae） constitutes an effective additive for animal production;its probiotic effect may be related to the concentrate-to-forage ratio（CTFR）.The objective of this study was to assess the effects of S.cerevisiae（SC） on fiber degradation and rumen microbial populations in steers fed diets with different levels of dietary concentrate.Ten Simmental × Local crossbred steers（450 ± 50 kg BW） were assigned to a control group or an SC group.Both groups were fed the same basal diet but the SC group received SC supplementation（8 × 10^9 cfu/h/d through the ruminal fistula）following a two-period crossover design.Each period consisted of four phases,each of which lasted 17 d：10 d for dietary adaptation,6 d for degradation study,and 1 d for rumen sample collection.From the 1^（st） to the 4^（th） phase,steers were fed in a stepwise fashion with increasing CTFRs,i.e.,30：70,50：50,70：30,and 90：10.The kinetics of dry matter and fiber degradation of alfalfa pellets were evaluated;the rumen microbial populations were detected using real-time PCR.The results revealed no significant（P〉 0.05） interactions between dietary CTFR and SC for most parameters.Dietary CTFR had a significant effect（P〈 0.01） on degradation characteristics of alfalfa pellets and the copies of rumen microorganism;the increasing concentrate level resulted in linear,quadratic or cubic variation trend for these parameters.SC supplementation significantly（P〈 0.05） affected dry matter（DM） and neutral detergent fiber（NDF）degradation rates（c_（DM）,c_（NDF）） and NDF effective degradability（ED_（NDF））.Compared with the control group,there was an increasing trend of rumen fungi and protozoa in SC group（P 〈 0.1）;copies of total bacteria in SC group were significantly higher（P〈 0.05）.Additionally,percentage of Ruminobacter amylophilus was significantly lower（P〈 0.05）but percentage of Selenomonas ruminantium was significantly higher（P〈 0.05） in the SC group.In a word,dietary CTFR had a significant effect on degradation characteristics of forage and rumen microbial population.S.cerevisiae had positive effects on DM and NDF degradation rate or effective degradability of forage;S.cerevisiae increased rumen total bacteria,fungi,protozoa,and lactate-utilizing bacteria but reduced starch-degrading and lactate-producing bacteria.

Consideration of some key issues of carbon market development in China

The global carbon market has developed rapidly with two significant trends of globalization and financialization.Deriving economic interest is a nation driven-force behind the international climate negotiation and carbon market.According to deeply analyzed relationships between the carbon market and the key subjects of the climate negotiation,this article reveals that promoting the development of the global carbon market is one of the core interests of developed nations.Based on the background of international carbon market development and domestic carbon market pilots,four suggestions to the key issues of China's carbon market are provided.The first is that the goal of China's carbon market should be in line with and contribute to the national objectives and policies addressing climate change.The second is that the Chinese carbon market should mainly target the emission reduction of production-sectors,and contribute to their upgradation and transformation.The third is mat the development of the nation-wide carbon market in China should first take the principle of unbalanced regional development into consideration.The fourth is that linking China's carbon market to the international market should keep steps in line with international opening-up of China's financing system.

溶瘤腺病毒CD55-TRAIL-IETD-MnSOD联合阿霉素抑制肝癌细胞HepG2生长

目的:研究溶瘤腺病毒CD55-TRAIL-IETD-MnSOD与阿霉素联合使用对肝癌细胞HepG2生长的影响。方法:MTT法检测经CD55-TRAIL-IETD-MnSOD与阿霉素联合处理后HepG2细胞生存率的变化,Hoechst33342染色及流式细胞术检测联合处理诱导细胞凋亡时细胞形态变化及凋亡细胞数量,Western印迹检测联合处理所引发的凋亡通路。结果:MTT实验显示,联合使用CD55-TRAIL-IETD-MnSOD和阿霉素能更有效地抑制HepG2细胞的生长,且阿霉素与CD55-TRAIL-IETD-MnSOD是协同作用;Hochest染色实验和流式细胞术实验结果均显示联合使用CD55-TRAIL-IETD-MnSOD和阿霉素增强了诱导细胞凋亡的作用;Western印迹表明CD55-TRAIL-IETD-MnSOD和阿霉素联合使用激活了Caspase细胞凋亡途径。结论:阿霉素能促进溶瘤腺病毒CD55-TRAIL-IETD-MnSOD的复制,联合阿霉素和溶瘤腺病毒CD55-TRAIL-IETD-MnSOD可更有效激活Caspase细胞凋亡通路,抑制肝癌细胞HepG2生长。

Distinction between short-segment Barrett's esophageal and cardiac intestinal metaplasia

AIM: To investigate the roles of mucin histochemistry,cytokeratin 7/20 (CK7/20) immunoreactivity, clinical characteristics and endoscopy to distinguish shortsegment Barrett's esophageal (SSBE) from cardiac intestinal metaplasia (CIM).METHODS: High iron diamine/Alcian blue (HID/AB)mucin-histochemical staining and immunohistochemical staining were used to classify intestinal metaplasia (IM)and to determine CK7/20 immunoreactivity pattern in SSBE and CIM, respectively, and these results were compared with endoscopical diagnosis and the positive rate of gastroesophageal reflux disease (GERD)symptoms and H pylori infection. Long-segment Barrett's esophageal and IM of gastric antrum were designed as control.RESULTS: The prevalence of type Ⅲ IM was significantly higher in SSBE than in CIM (63.33% vs23.08%, P＜0.005). The CK7/20 immunoreactivity in SSBE showed mainly Barrett's pattern (76.66%), and the GERD symptoms in most cases which showed Barrett's pattern were positive, whereas H pylori infection was negative. However, the CK7/20 immunoreactivity in CIM was gastric pattern preponderantly (61.54%), but there were 23.08% cases that showed Barrett's pattern. H pylori infection in all cases which showed gastric pattern was significantly higher than those which showed Barrett's pattern (63.83% vs 19.30%, P＜0.005), whereas the GERD symptoms in gastric pattern were significantly lower than that in Barrett's pattern (21.28% vs 85.96%,P＜0.005).CONCLUSION: Distinction of SSBE from CIM should not be based on a single method;however, the combination of clinical characteristics, histology, mucin histochemistry,CK7/20 immunoreactivity, and endoscopic biopsy should be applied. Type Ⅲ IM, presence of GERD symptoms,and Barrett's CK7/20 immunoreactivity pattern may support the diagnosis of SSBE, whereas non-type Ⅲ IM, positive H pylori infection, and gastric CK7/20immunoreactivity pattern may imply CIM.

Review on localized boundary integral equation:Discrete wavenumber method for 2D irregular layers

The pioneer study of simulating the wave field in media with irregular interface belongs to Aki and Lamer. Since that many numerical methods on the subject have been developed, such as pure numerical techniques, ray method and boundary method. The boundary method based on boundary integral equation is a semi-analytical method which is suitable to modeling wave field induced by irregular border. According to the property of the applied Green＇s function the boundary methods can be sorted into space domain boundary method and wavenumber domain boundary method. For both of them it is necessary to solve a large equation, which means much computation is needed. Thus, it is difficult for the boundary methods to be applied in simulating wave field with high frequency or in large range. To develop a new method with less computation is meaningful. For this purpose, localized boundary integral equation, i.e., discrete wavenumber method is proposed. It is rooted in the Bouchon-Campillo method, an important wavenumber domain boundary method. Firstly the force on interface is separated into two parts： one is on flat part and the other on irregular part of the interface. Then Fourier transform is applied to identify their relation, the unknown distributes only on irregular part. Consequently computation efficiency is dramatically improved. Importantly its accuracy is the same as that of Bouchon-Campillo.

Ocular Trauma as the First Presentation of Langerhans Cell Histiocytosis

Purpose:Ocular trauma occurs disproportionately in children on an annual basis.However,because of this frequency,other diagnoses,.such as orbital neoplasms,.can easily be ignored.Methods:We report on a two-year-old boy who presented with a dark purple,irregularly shaped lesion on his lower left eyelid.The patient had suffered injuries twice to that area.Results:Axial computed tomography images demonstrated an ill-defined and inhomogeneous soft tissue mass in the lateral and posterior region of the left orbit,.with bony destruction and absorption of the adjacent orbital wall..Magnetic resonance imaging of the orbit showed a lesion involving the greater sphenoidal wing,the supraorbital wall,and the frontal area.Enlarged nuchal and inguinal lymph nodes were detected by sonographic examination.Histopathological examination of a surgical biopsy showed an accumulation of characteristic Langerhans cells.The diagnosis of Langerhans cell histiocytosis was confirmed by immunohistochemical examinations positive for CD1a and SP-100.Conclusion:Orbital Langerhans cell histiocytosis is rarely encountered in ophthalmic practice,.so the ophthalmologist needs to be familiar with its presentation and work-up,.and has to be aware of possible OLCH diagnosis when a child(or even an adult)presents with prolonged and persistent eyelid edema,.even with a history of ocular trauma.

Cotton GhBRC1 regulates branching,flowering,and growth by integrating multiple hormone pathways

Cotton architecture is partly determined by shoot branching and flowering patterns.Gh BRC1 was previously identified by RNA-seq analysis of nulliplex-branching and normal-branching cotton.However,the roles of Gh BRC1 in cotton remain unclear.In the present study,investigations of nuclear localization and transcriptional activity indicated that Gh BRC1 has characteristics typical of transcription factors.Gene expression analysis showed that Gh BRC1 was highly expressed in axillary buds but displayed different expression patterns between the two branching types.Overexpression of Gh BRC1 in Arabidopsis significantly inhibited the number of branches and promoted flowering.In contrast,silencing Gh BRC1 in cotton significantly promoted seedling growth.Gh BRC1 was induced by multiple hormones,including strigolactones,which promoted seedling growth and seed germination of Arabidopsis plants overexpressing Gh BRC1.Consistent with these findings,RNA-seq analysis of virus-induced gene silencing treated cotton revealed that a large number of genes were differentially expressed between Gh BRC1-silenced and control plants,and these genes were significantly enriched in plant hormone signalling pathways.Together,our data indicates that Gh BRC1 regulates plant branching and flowering through multiple regulatory pathways,especially those regulating plant hormones,with functions partly differing from those of Arabidopsis BRC1.These results provide insights into the molecular mechanisms controlling plant architecture,which is important for breeding cotton with ideal plant architecture and high yield.

Optimize the Deployment and Integration for Multicast-Oriented Virtual Network Function Tree

Due to the development of network technology,the number of users is increasing rapidly,and the demand for emerging multicast services is becoming more and more abundant,traffic data is increasing day by day,network nodes are becoming denser,network topology is becoming more complex,and operators’equipment operation and maintenance costs are increasing.Network functions virtualization multicast issues include building a traffic forwarding topology,deploying the required functions,and directing traffic.Combining the two is still a problem to be studied in depth at present,and this paper proposes a two-stage solution where the decisions of these two stages are interdependent.Specifically,this paper decouples multicast traffic forwarding and function delivery.The minimum spanning tree of traffic forwarding is constructed by Steiner tree,and the traffic forwarding is realized by Viterbi-algorithm.Use a general topology network to examine network cost and service performance.Simulation results show that this method can reduce overhead and delay and optimize user experience.

Therapeutic Analysis of Fuzi Lizhong Wan with Trimebutine in the Treatment of Diarrhea Type lrritable Bowel Syndrome

Objective:To evaluate the clinical efficacy ofFuzi Lizhong Wan and trimebutine in the treatment of diarrhea irritab1e bowel syndrome.Methods:A tota1 of 90 patients with diarrhea irritable Dow1 syndrome were divided intocontro1 group(n=45)and treatment group(n=45)using the random.number table method.Patients in the control group were po administered withtrimebutine 0.2 g.three time daily for 4 weeks.Patients in the treatment group were po administered with Fuzi Lizhong Wan 9 g,three time daily for 4 weeks on the basis of the control group.The clinical efficacy and the TCM symptom scores were evaluated after treatment.The serological indicators in two groups were compared before and after treatment.Results:After treatment,the clinica1 efficacy and the TCM symptom scores in treatment group were significantly 1ower than that in contro1 group(P<0.05).The 1evels of serological indicators in both groups improved significant1y,and those in the treatment group were better than the control group(P<0.05).Conclusion:Combined therapy of Fuzi Lizhong Wan and trimebutine in the treatment of diarrhea type irritable bowe1 syndrome has a significant effect,which canrelieve clinical symptom;and reduce visceral sensitivity,and improve leve1s of inflammatory and quality of life.So it is suggested to be applied.

The Clinical Effects of Hyperbaric Oxygen in the Treatment of Sudden Deafness

Objective:To observe the clinical effect of hyperbaric oxygen in the treatment of sudden deafness.Methods:Ninety-six patients with sudden deafness diagnosed by the otolaryngology department were divided into 2 groups which comprised of 48 patients in the conventional treatment group and the other 48 patients in the hyperbaric oxygen treatment group.Both groups were treated with methylcobalamin,vitamin B1,and ginkgo biloba extract.The patients in the hyperbaric oxygen treatment group were given hyperbaric oxygen therapy of 2.0 ATA once a day.Each course of treatment lasted 10 days and after two courses of treatment,the clinical efficacies of the two groups were compared.Results:After two courses of treatment,the effect of treatment in the hyperbaric oxygen treatment group was significantly better than that of the conventional treatment group.Conclusion:Hyperbaric oxygen therapy can effectively improve the hearing level of patients with sudden deafness and the overall effective rate of treatment.

基于角度分辨倾斜背散射拉曼光谱的单晶硅应力分量解耦分析

对于应变工程而言,应力是控制半导体器件光电性能的关键.显微拉曼光谱是一种有效的半导体材料应力无损分析技术.然而,使用传统拉曼方法得到的结果通常是特定晶体/特定晶面的面内等效应力或主应力和.本文提出了一种基于角度分辨倾斜背散射显微拉曼光谱的应力分析方法.通过在样品坐标系中求解晶格动力学方程,在特征向量坐标系中进行拉曼选择,提出了一种通用的物理力学模型.利用该模型,考虑折射率、转偏和数值孔径影响等因素,建立了任意应力状态下任意晶面(以单晶硅{100}面为例)上偏振拉曼频移与各应力分量的解析关系.验证实验表明,应力分量的分析结果与施加的应力状态非常吻合.证明了基于角度分辨倾斜背散射显微拉曼光谱的方法、模型和仪器,能够解决单晶硅特别是{100}晶面上的应力分量不能通过拉曼测量解耦的普遍问题.

Intrauterine Treatment of Monochorionic Triamniotic Triplet Pregnancy with Twin Reverse Arterial Perfusion Sequence

To editor:This study reports a pregnant woman who was naturally conceived with monochorionic triamniotic(MCTA)combined with twin reversed arterial perfusion(TRAP).An ultrasound revealed that one of the fetuses had a cardiac malformation,and a connection between the umbilical arteries was suspected.To reduce the acardiac fetus,radiofrequency ablation was performed at 17+3 weeks of pregnancy.At 36+4 weeks,two healthy infants were delivered via elective cesarean section.Postoperative examination revealed a direct connection between the umbilical arteries,with the placental perfusion acting as a direct pump.It is essential to identify the intrauterine status of the blood-supplying infant and vascular connections in order to achieve successful outcomes in pregnancies with complications,such as edema and heart failure.The studies involving human participants were reviewed and approved by Human Research Ethics Committee of Tianjin Central Hospital of Obstetrics and Gynecology.The patient provided her written informed consent to participate in this study.

Protein lipidation in health and disease:molecular basis,physiological function and pathological implication

Posttranslational modifications increase the complexity and functional diversity of proteins in response to complex external stimuli and internal changes.Among these,protein lipidations which refer to lipid attachment to proteins are prominent,which primarily encompassing five types including S-palmitoylation,N-myristoylation,S-prenylation,glycosylphosphatidylinositol(GPl)anchor and cholesterylation.Lipid attachment to proteins plays an essential role in the regulation of protein trafficking,localisation,stability,conformation,interactions and signal transduction by enhancing hydrophobicity.Accumulating evidence from genetic,structural,and biomedical studies has consistently shown that protein lipidation is pivotal in the regulation of broad physiological functions and is inextricably linked to a variety of diseases.Decades of dedicated research have driven the development of a wide range of drugs targeting protein lipidation,and several agents have been developed and tested in preclinical and clinical studies,some of which,such as asciminib and lonafarnib are FDA-approved for therapeutic use,indicating that targeting protein lipidations represents a promising therapeutic strategy.Here,we comprehensively review the known regulatory enzymes and catalytic mechanisms of various protein lipidation types,outline the impact of protein lipidations on physiology and disease,and highlight potential therapeutic targets and clinical research progress,aiming to provide a comprehensive reference for future protein lipidation research.

Understanding ultra-dispersed CeO_(x)modified iridium clusters as bifunction electrocatalyst for high-efficiency water splitting in acid electrolytes

Designing bifunctional catalysts with high catalytic activity and durability for electrochemical water splitting has become a promising approach to producing clean and sustainable hydrogen fuels.With high specific surface area and ultra-small size,metal clusters have become a kind of promising catalysts.By introducing Ce species,the noble metal iridium(Ir)can be electronically modulated,thus reduce the activation energy of intermediate,and improve the intrinsic catalytic activity for practical application.The performance of Ir_(3)CeO_(x)/C is more prominent among different feeding ratios of the precursor.The overpotential is only 299 mV for the oxygen evolution reaction(OER),and the overpotential is only23 mV for the hydrogen evolution reaction(HER)at 10 mA/cm^(2) in acid media.The Ir doped with appropriate amount of Ce can obtain excellent OER and HER performance and catalyze water splitting efficiently.This work provides a strategic approach to the formation of clusters of precious metals and rare earths.

国有企业内部控制建设现状及其优化思考

在当前内部控制建设浪潮下,越来越多的国有企业采用合理准确的内部控制评价来有效实现企业自我诊断,展现了内部控制在企业发展中的强大魅力。以国有企业内部控制建设现状为切入点,探索研究国有企业六步内控建设优化法,进而对国有企业内部控制建设进行反思,以期为内控建设优化提供参考。