Variations in the pathogenicity of Newcastle disease virus(NDV),the agent causing Newcastle disease,are associated with variants of different virulence.A few studies have characterized the expression of microRNAs(miRN...Variations in the pathogenicity of Newcastle disease virus(NDV),the agent causing Newcastle disease,are associated with variants of different virulence.A few studies have characterized the expression of microRNAs(miRNAs)in NDV-infected avian cells.Here,the expression of miRNAs in chicken embryo fibroblasts(CEFs)infected with Herts/33 and LaSota NDV strains(highly virulent and nonvirulent,respectively)was determined using RNA sequencing.miRNAs involved in NDV infection included 562 previously documented and 184 novel miRNAs.miRNA target genes involved transcription factors,cell apoptosis,ubiquitin-mediated proteolysis,and protein processing in the endoplasmic reticulum.Potential target genes associated with autophagy were verified by qRT-PCR.No studies have documented the miRNA profles of CEFs infected with NDVs variants.This study adds to our knowledge of the cellular miRNAs involved in NDV infection and the complex molecular mechanisms mediating virus-host interactions.The results of this study will aid the development of strategies against the chicken virus.展开更多
基金This work was financially supported by the National Natural Science Foundation of China(No.31800144 and No.32030108)the Natural Science Foundation of Shanghai(No.18ZR1448700)the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Science.
文摘Variations in the pathogenicity of Newcastle disease virus(NDV),the agent causing Newcastle disease,are associated with variants of different virulence.A few studies have characterized the expression of microRNAs(miRNAs)in NDV-infected avian cells.Here,the expression of miRNAs in chicken embryo fibroblasts(CEFs)infected with Herts/33 and LaSota NDV strains(highly virulent and nonvirulent,respectively)was determined using RNA sequencing.miRNAs involved in NDV infection included 562 previously documented and 184 novel miRNAs.miRNA target genes involved transcription factors,cell apoptosis,ubiquitin-mediated proteolysis,and protein processing in the endoplasmic reticulum.Potential target genes associated with autophagy were verified by qRT-PCR.No studies have documented the miRNA profles of CEFs infected with NDVs variants.This study adds to our knowledge of the cellular miRNAs involved in NDV infection and the complex molecular mechanisms mediating virus-host interactions.The results of this study will aid the development of strategies against the chicken virus.
