We have shown that platelet-derived microvesicles(PMVs)induce abnormal proliferation,migration,and energy metabolism of vascular smooth muscle cells(VSMCs)after vascular intimal injury.Here,we examined a novel role of...We have shown that platelet-derived microvesicles(PMVs)induce abnormal proliferation,migration,and energy metabolism of vascular smooth muscle cells(VSMCs)after vascular intimal injury.Here,we examined a novel role of podosome in mediating matrix metalloproteinase-9(MMP-9)dependent VSMC migration induced by plateletderived microvesicles(PMVs).VSMCs were isolated from the thoracic aortas of male Sprague Dawley(SD)rats and identified with immunofluorescent staining.Blood samples were collected from SD Rats,the platelets were isolated with density gradient centrifugation from the blood samples and activated by collagen I.Intriguingly,proteins expressed in platelets were found to participate in the positive regulation of podosome assembly using GO analysis by DAVID,and most of the proteins were found in extracellular exosomes.Of note,activated platelets indirectly induced VSMC migration via releasing PMVs which was verified using platelets and VSMCs transwell coculture system.Besides,podosome,an invasive protrusion to mediate extracellular matrix(ECM)remodeling,was formed in VSMCs to induce cell migration.Furthermore,MMP-9 activity detected by gelatin zymography was used to verify the function of the podosome in ECM remodeling.The result indicated that MMP-9 activity was robustly activated in VSMCs to implement the function of the podosome.In addition,gelatin degradation was detected in intact VSMCs using a gelatin degradation assay after co-culture with platelets.Taken together,our data reveal a novel mechanism that PMVs promote VSMC migration via forming podosomes and inducing MMP-9 activity.展开更多
基金supported by grants from the National Natural Science Foundation of China[grant numbers 12032003 and 12102261].
文摘We have shown that platelet-derived microvesicles(PMVs)induce abnormal proliferation,migration,and energy metabolism of vascular smooth muscle cells(VSMCs)after vascular intimal injury.Here,we examined a novel role of podosome in mediating matrix metalloproteinase-9(MMP-9)dependent VSMC migration induced by plateletderived microvesicles(PMVs).VSMCs were isolated from the thoracic aortas of male Sprague Dawley(SD)rats and identified with immunofluorescent staining.Blood samples were collected from SD Rats,the platelets were isolated with density gradient centrifugation from the blood samples and activated by collagen I.Intriguingly,proteins expressed in platelets were found to participate in the positive regulation of podosome assembly using GO analysis by DAVID,and most of the proteins were found in extracellular exosomes.Of note,activated platelets indirectly induced VSMC migration via releasing PMVs which was verified using platelets and VSMCs transwell coculture system.Besides,podosome,an invasive protrusion to mediate extracellular matrix(ECM)remodeling,was formed in VSMCs to induce cell migration.Furthermore,MMP-9 activity detected by gelatin zymography was used to verify the function of the podosome in ECM remodeling.The result indicated that MMP-9 activity was robustly activated in VSMCs to implement the function of the podosome.In addition,gelatin degradation was detected in intact VSMCs using a gelatin degradation assay after co-culture with platelets.Taken together,our data reveal a novel mechanism that PMVs promote VSMC migration via forming podosomes and inducing MMP-9 activity.
