MicroRNA(miR)-200b-3p has been associated with many tumors,but its involvement in pituitary adenoma is unclear.This study investigated the molecular mechanism underlying miR-200b-3p regulation in pituitary adenomas to...MicroRNA(miR)-200b-3p has been associated with many tumors,but its involvement in pituitary adenoma is unclear.This study investigated the molecular mechanism underlying miR-200b-3p regulation in pituitary adenomas to provide a theoretical basis for treatment.Bioinformatics was used to analyze pituitary adenoma-related genes and screen new targets related to RECK and miRNA.As well,the relationship between miR-200b-3p and RECK protein was verified using a double-luciferase reporter gene assay.The expression of miR-200b-3p in clinical samples was analyzed by in situ hybridization.Transfection of the miR-200b-3p inhibitor and small interfering-RECK(si-RECK)was verified by qPCR.GH3 cell viability and proliferation were detected using CCK8 and EdU assays.Apoptosis was detected by flow cytometry and western blotting.Wound healing and Transwell assays were used to detect cell migration and invasion.The effects of miR-200b-3p and RECK on GH3 cells were verified using salvage experiments.miR-200b-3p was highly expressed in pituitary tumor tissue.Inhibitors of miR-200b-3p inhibited cell proliferation promoted cell apoptosis,inhibited invasion and migration,and inhibited the expression of matrix metalloproteinases.Interestingly,miR-200b-3p negatively regulated RECK.The expression of RECK in pituitary adenoma tissues was lower than that in neighboring tissues.Si-RECK rescued the function of miR-200b-3p inhibitors in the above cellular behaviors,and miR-200b-3p accelerated the development of pituitary adenoma by negatively regulating RECK expression.In summary,this study investigated the molecular mechanism by which miR-200b-3p regulates the progression of pituitary adenoma through the negative regulation of RECK.The findings provide a new target for the treatment of pituitary adenoma.展开更多
Chaihu plus Longgu Muli Decoction is the representative treatment method of harmonizing and releasing combined with easing for lesser yang disease proposed by famous physician Zhang Zhongjing.Through clinical cases,th...Chaihu plus Longgu Muli Decoction is the representative treatment method of harmonizing and releasing combined with easing for lesser yang disease proposed by famous physician Zhang Zhongjing.Through clinical cases,this paper firstly introduced the main framework of Chaihu plus Longgu Muli Decoction,to make the treatment method of harmonizing and releasing become more flexible in clinical application.Besides,it introduced the common changes in the learning and application of the decoction,to respond to various changes in the clinical diseases.展开更多
Semiconductor optical amplifier-based ring cavity laser (SOA-RL), which has been widely used in optical communications, optical fiber sensing, and biophotonics fields, can be tuned at an ultra high speed up to Mega He...Semiconductor optical amplifier-based ring cavity laser (SOA-RL), which has been widely used in optical communications, optical fiber sensing, and biophotonics fields, can be tuned at an ultra high speed up to Mega Hertz over 100 nm bandwidth range with high SNR and flatness output. A steady-state model and segmentation algorithms are employed to investigate the gain spectra of four kinds of non-uniform SOA and the lasing wavelength of the SOA-RL. It shows that the dependence of the lasing wavelength on the average width is stronger when the light propagates from narrower to wider end than conversely, and there are some particular structures to show ultra high stability lasing wavelength. It is supposed that the main reason could be the carrier density distribution along the propagation.展开更多
Experiments and simulations demonstrate that an SOA-based ring cavity can operate as a tunable laser, wavelength- swept laser or Fourier-domain-mode-locking laser according to the relation between the roundtrip freque...Experiments and simulations demonstrate that an SOA-based ring cavity can operate as a tunable laser, wavelength- swept laser or Fourier-domain-mode-locking laser according to the relation between the roundtrip frequency and the sweeping frequency of the filter.展开更多
The Tianlai Cylinder Pathfinder is a radio interferometer array designed to test techniques for 21 cm intensity mapping in the post-reionization Universe,with the ultimate aim of mapping the large scale structure and ...The Tianlai Cylinder Pathfinder is a radio interferometer array designed to test techniques for 21 cm intensity mapping in the post-reionization Universe,with the ultimate aim of mapping the large scale structure and measuring cosmological parameters such as the dark energy equation of state.Each of its three parallel cylinder reflectors is oriented in the north-south direction,and the array has a large field of view.As the Earth rotates,the northern sky is observed by drift scanning.The array is located in Hongliuxia,a radio-quiet site in Xinjiang,and saw its first light in September 2016.In this first data analysis paper for the Tianlai cylinder array,we discuss the sub-system qualification tests,and present basic system performance obtained from preliminary analysis of the commissioning observations during 2016-2018.We show typical interferometric visibility data,from which we derive the actual beam profile in the east-west direction and the frequency band-pass response.We describe also the calibration process to determine the complex gains for the array elements,either using bright astronomical point sources,or an artificial on site calibrator source,and discuss the instrument response stability,crucial for transit interferometry.Based on this analysis,we find a system temperature of about 90 K,and we also estimate the sensitivity of the array.展开更多
基金supported by Correlation between RECK and GH-type pituitary adenomas(No.21JR11RE027).
文摘MicroRNA(miR)-200b-3p has been associated with many tumors,but its involvement in pituitary adenoma is unclear.This study investigated the molecular mechanism underlying miR-200b-3p regulation in pituitary adenomas to provide a theoretical basis for treatment.Bioinformatics was used to analyze pituitary adenoma-related genes and screen new targets related to RECK and miRNA.As well,the relationship between miR-200b-3p and RECK protein was verified using a double-luciferase reporter gene assay.The expression of miR-200b-3p in clinical samples was analyzed by in situ hybridization.Transfection of the miR-200b-3p inhibitor and small interfering-RECK(si-RECK)was verified by qPCR.GH3 cell viability and proliferation were detected using CCK8 and EdU assays.Apoptosis was detected by flow cytometry and western blotting.Wound healing and Transwell assays were used to detect cell migration and invasion.The effects of miR-200b-3p and RECK on GH3 cells were verified using salvage experiments.miR-200b-3p was highly expressed in pituitary tumor tissue.Inhibitors of miR-200b-3p inhibited cell proliferation promoted cell apoptosis,inhibited invasion and migration,and inhibited the expression of matrix metalloproteinases.Interestingly,miR-200b-3p negatively regulated RECK.The expression of RECK in pituitary adenoma tissues was lower than that in neighboring tissues.Si-RECK rescued the function of miR-200b-3p inhibitors in the above cellular behaviors,and miR-200b-3p accelerated the development of pituitary adenoma by negatively regulating RECK expression.In summary,this study investigated the molecular mechanism by which miR-200b-3p regulates the progression of pituitary adenoma through the negative regulation of RECK.The findings provide a new target for the treatment of pituitary adenoma.
文摘Chaihu plus Longgu Muli Decoction is the representative treatment method of harmonizing and releasing combined with easing for lesser yang disease proposed by famous physician Zhang Zhongjing.Through clinical cases,this paper firstly introduced the main framework of Chaihu plus Longgu Muli Decoction,to make the treatment method of harmonizing and releasing become more flexible in clinical application.Besides,it introduced the common changes in the learning and application of the decoction,to respond to various changes in the clinical diseases.
文摘Semiconductor optical amplifier-based ring cavity laser (SOA-RL), which has been widely used in optical communications, optical fiber sensing, and biophotonics fields, can be tuned at an ultra high speed up to Mega Hertz over 100 nm bandwidth range with high SNR and flatness output. A steady-state model and segmentation algorithms are employed to investigate the gain spectra of four kinds of non-uniform SOA and the lasing wavelength of the SOA-RL. It shows that the dependence of the lasing wavelength on the average width is stronger when the light propagates from narrower to wider end than conversely, and there are some particular structures to show ultra high stability lasing wavelength. It is supposed that the main reason could be the carrier density distribution along the propagation.
文摘Experiments and simulations demonstrate that an SOA-based ring cavity can operate as a tunable laser, wavelength- swept laser or Fourier-domain-mode-locking laser according to the relation between the roundtrip frequency and the sweeping frequency of the filter.
基金support of the Astronomical Technology Center of National Astronomical Observatories of China(NAOC)support of Ministry of Science and Technology(MOST)(Grant No.2012AA121701)+10 种基金supported by MOST(Grant Nos.2016YFE0100300,and 2018YFE0120800)the National Natural Science Foundation of China(NSFC)(Grant Nos.11633004,11473044,and 11653003)the Chinese Academy of Sciences(CAS)(Grant No.QYZDJ-SSW-SLH017)support of the NSFC-CAS Joint Fund of Astronomy(Grant No.U1631118)partially supported by the National Key R&D Program(Grant No.2017YFA0402603)the CAS Interdisciplinary Innovation Team(Grant No.JCTD-2019-05)support of NSFC(Grant No.U1501501)the Tianhe-1 supercomputerpartially supported by the US National Science Foundation(NSF)Award(Grant No.AST-1616554)partial support from Centre National de la Recherche Scientifique(CNRS)via IN2P3&INSU,Observatoire de ParisIrfu/CEA。
文摘The Tianlai Cylinder Pathfinder is a radio interferometer array designed to test techniques for 21 cm intensity mapping in the post-reionization Universe,with the ultimate aim of mapping the large scale structure and measuring cosmological parameters such as the dark energy equation of state.Each of its three parallel cylinder reflectors is oriented in the north-south direction,and the array has a large field of view.As the Earth rotates,the northern sky is observed by drift scanning.The array is located in Hongliuxia,a radio-quiet site in Xinjiang,and saw its first light in September 2016.In this first data analysis paper for the Tianlai cylinder array,we discuss the sub-system qualification tests,and present basic system performance obtained from preliminary analysis of the commissioning observations during 2016-2018.We show typical interferometric visibility data,from which we derive the actual beam profile in the east-west direction and the frequency band-pass response.We describe also the calibration process to determine the complex gains for the array elements,either using bright astronomical point sources,or an artificial on site calibrator source,and discuss the instrument response stability,crucial for transit interferometry.Based on this analysis,we find a system temperature of about 90 K,and we also estimate the sensitivity of the array.
