At present, transgenic technologies have become important means of plant breeding, and the application and promotion of transgenic technologies have created huge economic and social benefits. Transgenic plant products...At present, transgenic technologies have become important means of plant breeding, and the application and promotion of transgenic technologies have created huge economic and social benefits. Transgenic plant products have significantly affected human life. Anthurium andraeanum is the second major tropical potted flower and its transgenic breeding has a promising prospect of application. In this paper, acceptors, transformation methods and introduced exogenous genes （ including reporter genes, selectable marker genes and target genes） of Anthurium andraeanum were summarized; in addition, several issues related to transforma- tion of Anthurium andraeanum were analyzed, aiming at providing reference for transgenic breeding of Anthurium andraeanum.展开更多
In this study, an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established, with the tender bulbs as explants. And then the sensitivity of the explants and calli to kanamycin and ...In this study, an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established, with the tender bulbs as explants. And then the sensitivity of the explants and calli to kanamycin and hygromycin was evaluated. The results suggested that the embryogenic calli were induced from tender bulbs and cultured in Murashige and Skoog （MS） medium supplemented with 0.5 mg/L N-phenyl-N＇-1,2,3-thiadiazol-5-ylurea （TDZ）, 1.0 mg/L 2,4-diehloro- phenoxyacetic acid （2,4-D）, and 30 g/L sucrose （ pH5. 8） in the dark at 25 ±1℃. Further study of the influence of kanamyein and hygromycin on callus induction and multiplication showed that, the lethal doses of kanamycin and hygromycin to bulb explants were 100 and 30 mg/L, respectively. All explants of H. vittatum died on the medium supplemented with 100 mg/L kanamycin or 30 mg/L hygromycin at the induction stage, and callus proliferation was completely inhibited by 100 mg/L kanamycin or 25 mg,/L hygromyein, and all the calli died at last. These results will provide important reference for further studies of trausgenic H. vittaturn.展开更多
In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of ka...In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of kanamycin and hygromycin on survival rate of cell aggregates,somatic embryogenesis and plantlet regeneration of A.andraeanum.According to the results,at the embryonic cell propagation stage,lethal doses of kanamycin and hygromycin to embryogenic cell aggregates of A.andraeanum were 200 and 60 mg/L,respectively;at the differentiation stage,either 150 mg/L kanamycin or 40 mg/L hygromycin could inhibit somatic embryogenesis;either 100 mg/L kanamycin or 20 mg/L hygromycin could inhibit plantlet regeneration.These results provided important reference for further studies of transgenic A.andraeanum.展开更多
This paper proposes a novel method for calibrating error coefficients of the quartz accelerometer faster on the dynamic centrifuge,which can generate a continuous dynamic acceleration excitation.Firstly,working princi...This paper proposes a novel method for calibrating error coefficients of the quartz accelerometer faster on the dynamic centrifuge,which can generate a continuous dynamic acceleration excitation.Firstly,working principle and structure of the dynamic centrifuge are analyzed,the error sources and the uncertainty of the dynamic centrifuge are expounded,and relevant coordinate systems are established.Then,according to the characteristics of the input specific forces and the propagating methods of the error sources,the accurate input specific forces acted on the input reference axes of accelerometer are obtained.Based on the calibration error model of the accelerometer,the corresponding dynamic calibration method is proposed.Finally,the second-order and high-order coefficients of the acceleration error model are calibrated,and the calibration precision of the coefficients is analyzed.Compared with traditional calibration methods,the method proposed in this paper not only greatly enhances the calibration efficiency by reducing the installing positions of the accelerometer,but also separates the centrifuge errors from the outputs of the accelerometer,which improves the accuracy and efficiency of the calibration method simultaneously.展开更多
基金Supported by Special Foundation of President of Guangdong Academy of Agricultural Sciences(201217)
文摘At present, transgenic technologies have become important means of plant breeding, and the application and promotion of transgenic technologies have created huge economic and social benefits. Transgenic plant products have significantly affected human life. Anthurium andraeanum is the second major tropical potted flower and its transgenic breeding has a promising prospect of application. In this paper, acceptors, transformation methods and introduced exogenous genes （ including reporter genes, selectable marker genes and target genes） of Anthurium andraeanum were summarized; in addition, several issues related to transforma- tion of Anthurium andraeanum were analyzed, aiming at providing reference for transgenic breeding of Anthurium andraeanum.
基金Supported by Science and Technology Program of Guangzhou City(2016201604030076)Science and Technology Program of Guangdong Province(2014A020208062)+2 种基金Cooperation Project of Industry,Education and Academy of Dongguan City(2015509105105)the Fund for Demonstration and Extension of Introduced Intellectual Achievements in Guangdong "Forcing Cultivation and Flowering Regulation Technology Introduction,Demonstration and Promotion in Hippeastrum vittatum" and "The Characteristics of Taiwan Varieties and Cultivation Technology Introduction and Promotion in Hippeastrum vittatum"Open Project of Key Laboratory of Urban Agriculture in South China,Ministry of Agriculture,China
文摘In this study, an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established, with the tender bulbs as explants. And then the sensitivity of the explants and calli to kanamycin and hygromycin was evaluated. The results suggested that the embryogenic calli were induced from tender bulbs and cultured in Murashige and Skoog （MS） medium supplemented with 0.5 mg/L N-phenyl-N＇-1,2,3-thiadiazol-5-ylurea （TDZ）, 1.0 mg/L 2,4-diehloro- phenoxyacetic acid （2,4-D）, and 30 g/L sucrose （ pH5. 8） in the dark at 25 ±1℃. Further study of the influence of kanamyein and hygromycin on callus induction and multiplication showed that, the lethal doses of kanamycin and hygromycin to bulb explants were 100 and 30 mg/L, respectively. All explants of H. vittatum died on the medium supplemented with 100 mg/L kanamycin or 30 mg/L hygromycin at the induction stage, and callus proliferation was completely inhibited by 100 mg/L kanamycin or 25 mg,/L hygromyein, and all the calli died at last. These results will provide important reference for further studies of trausgenic H. vittaturn.
基金Supported by Special Fund of President of the Guangdong Academy of Agricultural Sciences(201217)
文摘In this study,embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum‘Alabama’were used as experimental materials to investigate the effects of kanamycin and hygromycin on survival rate of cell aggregates,somatic embryogenesis and plantlet regeneration of A.andraeanum.According to the results,at the embryonic cell propagation stage,lethal doses of kanamycin and hygromycin to embryogenic cell aggregates of A.andraeanum were 200 and 60 mg/L,respectively;at the differentiation stage,either 150 mg/L kanamycin or 40 mg/L hygromycin could inhibit somatic embryogenesis;either 100 mg/L kanamycin or 20 mg/L hygromycin could inhibit plantlet regeneration.These results provided important reference for further studies of transgenic A.andraeanum.
基金supported by the National Natural Science Foundation ofChina (Grant Nos. 60978014,61178022 and 11074027)the Basic Research Fund from Sci. & Tech. Department of Jilin Province (Grant Nos.20100521,20100168 and 20111812)
文摘This paper proposes a novel method for calibrating error coefficients of the quartz accelerometer faster on the dynamic centrifuge,which can generate a continuous dynamic acceleration excitation.Firstly,working principle and structure of the dynamic centrifuge are analyzed,the error sources and the uncertainty of the dynamic centrifuge are expounded,and relevant coordinate systems are established.Then,according to the characteristics of the input specific forces and the propagating methods of the error sources,the accurate input specific forces acted on the input reference axes of accelerometer are obtained.Based on the calibration error model of the accelerometer,the corresponding dynamic calibration method is proposed.Finally,the second-order and high-order coefficients of the acceleration error model are calibrated,and the calibration precision of the coefficients is analyzed.Compared with traditional calibration methods,the method proposed in this paper not only greatly enhances the calibration efficiency by reducing the installing positions of the accelerometer,but also separates the centrifuge errors from the outputs of the accelerometer,which improves the accuracy and efficiency of the calibration method simultaneously.
