[Objectives]Peptides was extracted from Haemadipsa hainana and its activity was studied.[Methods]Electric stimulation,water extraction and ultrasonic extraction were used to extract the peptides from H.hainana.Then th...[Objectives]Peptides was extracted from Haemadipsa hainana and its activity was studied.[Methods]Electric stimulation,water extraction and ultrasonic extraction were used to extract the peptides from H.hainana.Then the protein content and molecular weight distribution of H.hainana peptides were detected by the BCA method and SDS-PAGE method,respectively.The antithrombin activity and analgesic activity of the three peptide extracts of H.hainana were detected by Markwardt thrombin titration method and mouse hot plate experiment,respectively.[Results]There extraction methods of electric stimulation,water extraction and ultrasonic extraction were used to extract the peptide extract of H.hainana,and the yields were as follows:water extraction>electrical stimulation>ultrasonic extraction.The three peptide extracts from H.hainana had antithrombin activity,and the antithrombin activity was as follows:water extraction>ultrasonic extraction>electrical stimulation.Through the hot plate experiment in mice,it was verified that the three peptide extracts of H.hainana had analgesic activity,and the analgesic activity was water extraction>electric stimulation>ultrasonic extraction.The analgesic activity of high-dose(100 mg/kg)group of H.hainana obtained by water extraction was slightly weaker than that of tramadol.[Conclusions]This study confirmed that the peptide extract of H.hainana had certain antithrombin and analgesic activity,laying a foundation for the subsequent development and application of H.hainana.展开更多
Ocean waves can directly drive WECs （wave energy converters） to perform two types of motion--reciprocating motion and unidirectional rotary motion. In general, the efficiency of a reciprocating WEC is strongly wave-...Ocean waves can directly drive WECs （wave energy converters） to perform two types of motion--reciprocating motion and unidirectional rotary motion. In general, the efficiency of a reciprocating WEC is strongly wave-frequency dependent, whereas the efficiency of a rotary WEC can be somewhat wave-frequency independent. To date, a huge majority of WEC technologies under development in industry belong to the reciprocating class, and only a few WEC concepts fall in the unidirectional rotary class. In the present work, a wave-driven rotor for unidirectional rotary motion was proposed and characterized. A numerical tool has been developed for characterization of the rotor＇s unidirectional rotary tendency. The tool included a wave model and a drag force model. Simple circular tubes were used as blades in a basic rotor design. This basic design demonstrated strong potential for unidirectional rotary motion at a proper rotor submersion level and under various wave conditions. Two improved designs were yielded from the basic design. In one improved design, the original circular tubes were replaced with cylindrical shells of semicircular cross section as new blades. In another design, the semicircular shells were further modified to become one-way foldable. The two improvements significantly enhanced the rotors＇ unidirectional rotary tendency in waves, which has been verified by numerical simulation. Broad ranges of wave parameters and the submersion level have been numerically explored on the two improved rotor designs in conjunction with dimensional analysis.展开更多
基金Supported by"2020 Hainan Provincial Higher Education Teaching Reform Research and Scientific Research"Project of The Education Department of Hainan Province(Hnky2020-76)Research Fund of Hainan Health Management College(2020ZR01)。
文摘[Objectives]Peptides was extracted from Haemadipsa hainana and its activity was studied.[Methods]Electric stimulation,water extraction and ultrasonic extraction were used to extract the peptides from H.hainana.Then the protein content and molecular weight distribution of H.hainana peptides were detected by the BCA method and SDS-PAGE method,respectively.The antithrombin activity and analgesic activity of the three peptide extracts of H.hainana were detected by Markwardt thrombin titration method and mouse hot plate experiment,respectively.[Results]There extraction methods of electric stimulation,water extraction and ultrasonic extraction were used to extract the peptide extract of H.hainana,and the yields were as follows:water extraction>electrical stimulation>ultrasonic extraction.The three peptide extracts from H.hainana had antithrombin activity,and the antithrombin activity was as follows:water extraction>ultrasonic extraction>electrical stimulation.Through the hot plate experiment in mice,it was verified that the three peptide extracts of H.hainana had analgesic activity,and the analgesic activity was water extraction>electric stimulation>ultrasonic extraction.The analgesic activity of high-dose(100 mg/kg)group of H.hainana obtained by water extraction was slightly weaker than that of tramadol.[Conclusions]This study confirmed that the peptide extract of H.hainana had certain antithrombin and analgesic activity,laying a foundation for the subsequent development and application of H.hainana.
文摘Ocean waves can directly drive WECs （wave energy converters） to perform two types of motion--reciprocating motion and unidirectional rotary motion. In general, the efficiency of a reciprocating WEC is strongly wave-frequency dependent, whereas the efficiency of a rotary WEC can be somewhat wave-frequency independent. To date, a huge majority of WEC technologies under development in industry belong to the reciprocating class, and only a few WEC concepts fall in the unidirectional rotary class. In the present work, a wave-driven rotor for unidirectional rotary motion was proposed and characterized. A numerical tool has been developed for characterization of the rotor＇s unidirectional rotary tendency. The tool included a wave model and a drag force model. Simple circular tubes were used as blades in a basic rotor design. This basic design demonstrated strong potential for unidirectional rotary motion at a proper rotor submersion level and under various wave conditions. Two improved designs were yielded from the basic design. In one improved design, the original circular tubes were replaced with cylindrical shells of semicircular cross section as new blades. In another design, the semicircular shells were further modified to become one-way foldable. The two improvements significantly enhanced the rotors＇ unidirectional rotary tendency in waves, which has been verified by numerical simulation. Broad ranges of wave parameters and the submersion level have been numerically explored on the two improved rotor designs in conjunction with dimensional analysis.
