Objective: The aim of the study was to improve the therapeutic effect for lung cancer using a synergetic strategy of adenovirus-based gene therapy combined with chemotherapy. Methods: A conditional replication adenovi...Objective: The aim of the study was to improve the therapeutic effect for lung cancer using a synergetic strategy of adenovirus-based gene therapy combined with chemotherapy. Methods: A conditional replication adenovirus(CRAd) was employed to treat the A549 lung cancer cells and cisplatin-resisted A549(A549-DDP) cells. In vitro MTS / PMS assay were used to evaluate the cell viability. PCR were used to detect expression of Coxsackie receptor (CAR) and Multidrug resistance(MDR) gene. The in vivo anti-tumor effect of CRAd and cisplatin was evaluated using a subcutaneous mouse model. Results: The CRAd sensitizes A549 cancer cells to cisplatin. The mechanism of enhanced cell growth inhibition is associated with the increased CAR and MDR expression. Conclusion: Our approach is better than the conventional gene therapy and chemotherapy strategy.展开更多
Object: To explore the therapeutic effects and therapeutic mechanisms of Conditional Replication Adenovirus(CRAd) in combination with cisplatin on lung cancer cells. Methods: Using MTS / PMS assay, in vitro cell inhib...Object: To explore the therapeutic effects and therapeutic mechanisms of Conditional Replication Adenovirus(CRAd) in combination with cisplatin on lung cancer cells. Methods: Using MTS / PMS assay, in vitro cell inhibition assay was performed to detect the cell viability of two lung cancer cell lines, NCI-H292 and NCI-H661. PCR was employed to detect the Coxsackie receptor(CAR) expression of cancer cells. The in vivo anti-tumor effect of CRAd and cisplatin was evaluated using a subcutaneous mouse model. Results: The CRAd with cisplatin is superior to the use of cisplatin or CRAd viruse alone on the suppression of lung cancer cell growth. The mechanism of inhibition is associated with the increased CAR expression. Conclusion: The application of CRAd in combination with cisplatin could play a better therapeutic effect on lung cancer cell growth inhibition.展开更多
Cancer stem cells(CSCs)are widely consid-ered to be a small cell population in leukemia and many solid cancers with the properties including self-renewal and differentiation to non-tumorigenic cancer cells.Identificati...Cancer stem cells(CSCs)are widely consid-ered to be a small cell population in leukemia and many solid cancers with the properties including self-renewal and differentiation to non-tumorigenic cancer cells.Identification and isolation of CSCs significantly depend on the special surface markers of CSCs.Aberrant gene expression and signal transduction contribute to malig-nancies of CSCs,which result in cancer initiation,progression and recurrence.The inefficient therapy of cancers is mainly attributed to the failure of elimination of the malignant CSCs.However,CSCs have not been detected in all cancers and hierarchical organization of tumors might challenge cancer stem cell models.Addi-tionally,opinions about the validity of the CSC hypothesis,the biological properties of CSCs,and the relevance of CSCs to cancer therapy differ widely.In this review,we discuss the debate of cancer stem cell model,the parameters by which CSCs can or cannot be defined,and the advances in the therapy of CSCs.展开更多
文摘Objective: The aim of the study was to improve the therapeutic effect for lung cancer using a synergetic strategy of adenovirus-based gene therapy combined with chemotherapy. Methods: A conditional replication adenovirus(CRAd) was employed to treat the A549 lung cancer cells and cisplatin-resisted A549(A549-DDP) cells. In vitro MTS / PMS assay were used to evaluate the cell viability. PCR were used to detect expression of Coxsackie receptor (CAR) and Multidrug resistance(MDR) gene. The in vivo anti-tumor effect of CRAd and cisplatin was evaluated using a subcutaneous mouse model. Results: The CRAd sensitizes A549 cancer cells to cisplatin. The mechanism of enhanced cell growth inhibition is associated with the increased CAR and MDR expression. Conclusion: Our approach is better than the conventional gene therapy and chemotherapy strategy.
文摘Object: To explore the therapeutic effects and therapeutic mechanisms of Conditional Replication Adenovirus(CRAd) in combination with cisplatin on lung cancer cells. Methods: Using MTS / PMS assay, in vitro cell inhibition assay was performed to detect the cell viability of two lung cancer cell lines, NCI-H292 and NCI-H661. PCR was employed to detect the Coxsackie receptor(CAR) expression of cancer cells. The in vivo anti-tumor effect of CRAd and cisplatin was evaluated using a subcutaneous mouse model. Results: The CRAd with cisplatin is superior to the use of cisplatin or CRAd viruse alone on the suppression of lung cancer cell growth. The mechanism of inhibition is associated with the increased CAR expression. Conclusion: The application of CRAd in combination with cisplatin could play a better therapeutic effect on lung cancer cell growth inhibition.
基金supported by National Basic Research Program(973 Project)of China(No.2009CB918902),Hundreds of Talents Program of Chinese Academy of Sciences 2009–2014,and a grant from Beijing Institutes of Life Science,Chinese Academy of Sciences.
文摘Cancer stem cells(CSCs)are widely consid-ered to be a small cell population in leukemia and many solid cancers with the properties including self-renewal and differentiation to non-tumorigenic cancer cells.Identification and isolation of CSCs significantly depend on the special surface markers of CSCs.Aberrant gene expression and signal transduction contribute to malig-nancies of CSCs,which result in cancer initiation,progression and recurrence.The inefficient therapy of cancers is mainly attributed to the failure of elimination of the malignant CSCs.However,CSCs have not been detected in all cancers and hierarchical organization of tumors might challenge cancer stem cell models.Addi-tionally,opinions about the validity of the CSC hypothesis,the biological properties of CSCs,and the relevance of CSCs to cancer therapy differ widely.In this review,we discuss the debate of cancer stem cell model,the parameters by which CSCs can or cannot be defined,and the advances in the therapy of CSCs.
