Pharmacodynamics Study in vitro and in vivo of Zijin Huadu Suppository Treating Cervicitis

[Objectives] To observe the pharmaceutical effect of Zijin Huadu suppository on treatment of cervicitis induced by virus,bacteria and phenol in vivo and in vitro,and provide experimental basis for evaluating its therapeutic effects for cervicitis. [Methods]Real-time RT-PCR and CPE methods were used to observe the inhibition of Zijin Huadu suppository on virus,and turbidimetry was used to observe bacteriostatic action; HE pathological section was used to observe cervicitis of rat,and enzyme-linked immunosorbent assay was used to detect virus' s expression in cervical tissue.[Results]In cervicitis rat model infected by HPV16,Zijin Huadu suppositories at the doses of 1. 2,0. 6 and0. 3 g/( kg·d) had significant inhibitory effect on HPV16 expression in cervical tissues,and the doses of 1. 2 and 0. 6 g/( kg·d) had significantly inhibitory effect on pathological changes of cervical tissues. In cervicities rat models infected mixedly by Escherichia coli,Neisseria gonorrhoeae and Staphylococcus aureus,Zijin Huadu suppositories at the doses of 1. 2 and 0. 6 g/( kg·d) had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models induced by chemical substances,Zijin Huadu suppositories at the dose of1. 2 g/( kg·d) had significant inhibitory effect on vagina and cervix lesions in rats. In vitro,Zijin Huadu suppository showed obvious inhibitory effects on HSV-2,HPV16,Staphylococcus aureus,Staphylococcus albus,Staphylococcus epidermidis,group B Streptococcus,E. coli,Pseudomonas aeruginosa,Proteusbacillus vulgaris,Neisseria gonorrhoeae and Candida albicans.[Conclusions] Zijin Huadu suppository has obvious inhibitory effect in vitro on viruses and bacteria,and could obviously improve the phenol-induced cervicitis.

Antidepressant Effect of Shenwei Ningyu Tablets on Rat Chronic Stress Model and Mouse Tail Suspension Model

[Objectives]To explore the antidepressant effect of Shenwei Ningyu Tablet,a new antidepressant traditional Chinese medicine,on rat chronic stress depression model and mouse tail suspension models.[Methods]Rat chronic stress model:except for the normal group,the rats in other groups were given corresponding chronic stress,and administered by gavage 1 h before modeling,for a total of 21 d.The changes of each indicator before and after the experiment were observed through the body weight change,the sugar water test,and open field test.The relevant hormone levels were detected by radioimmunoassay.Mouse tail suspension depression model:after continuous administration for 7 d,the activity times was recorded with the mouse automatic recorder,and the mouse immobility time was recorded after tail suspension,to explore the effects of each administration group on the tail suspension immobility time of mice.[Results]Chronic stress depression model:21 d after modeling,compared with the normal group,rats in the model group exhibited significantly reduced body weight,sucrose preference index,and horizontal and vertical movement scores(P<0.05).Compared with the model group,the low-dose Shenwei Ningyu Tablets group had significant differences in the sugar water test,horizontal and vertical movement scores(P<0.05).In addition,all three dose groups of Shenwei Ningyu Tablets could effectively reduce the content of CRF in chronic stress model rats,and the low dose group could significantly reduce the ACTH level in model rats(P<0.05).Mouse tail suspension depression model:the immobility time after tail suspension in each administration group was significantly different from that in the model group(P<0.05).[Conclusions]Shenwei Ningyu Tablets has a certain anti-depression effect on both the rat chronic stress depression model and the mouse tail suspension depression model.

Feedforward loop profile among transcription factor, miRNA and mRNA in influenza A virus-infected mouse lungs

Purpose:In the present study,we focused on the 46 microRNAs and 719 genes in the microRNA-gene network,reported by us,and aimed to build a research blueprint of feedforward loops and reveal the key TFs in H1N1-infected mouse lung.Method:Based on microRNAs and genes in the microRNA-gene network previously reported by us,we used Jemboss software to find relationships between TFs and microRNAs(or genes),and then built a TF-microRNA-gene network exploiting the interactions between TFs and microRNAs(or genes).Next,we searched the sequences of above genes or microRNAs near the transcription start site(TSS)area,and then used the MatchTM algorithm to predict relevant TFs,and built the TF-Gene-Network.Result:We built a TF-microRNAgene network and exploreed eight key TFs,namely NF-AT1,GKLF,SRY,SOX10,AML1,MZF1,CRX and myogenin,in the network,and then constructed subgraphs of these eight TFs.Simultaneously,we predicted the possible target genes of microRNAs and identified the feedforward regulation relationship of possible TFs,microRNAs and mRNAs.The results showed that all eight factors with a score greater than 100 were TFs,namely NF-AT1,GKLF,SRY,SOX10,AML1,CRX,myogenin and MZF1.We then constructed subtables of the above eight TFs.Conclusion:In this study,TFs including NF-AT1,GKLF,SRY,SOX10,AML1,MZF1,CRX and myogenin showed the highest score(>100)not only in the TF-microRNA-gene network but also in feedforward loops,indicating that these eight TFs play the most important roles in mouse H1N1 influenza virus infection biology.

VWF基因突变导致混合痔术后大出血一例报告并文献复习

本文报告以混合痔术后反复大出血,常规凝血检查无异常,最终依据出凝血基因检测以及家系调查得以确诊为遗传性血管性血友病的诊治过程,并文献复习。以期提高临床医生对血管性血友病的深层认知,开拓诊疗思路,降低不良事件的发生。

Antiviral activity of Jinchai capsule against influenza virus

OBJECTIVE:To evaluate the effect on influenza virus of Jinchai,a capsule made of Traditional Chinese Medicine.METHODS:Madin-darby canine kidney(MDCK) cells were infected with the FM1 strain of influenza virus A(subtype H1N1) in vitro.They were used to explore how Jinchai affected cell adsorption,cell membrane fusion,transcription and replication of the influenza virus.Hemagglutinin(HA) protein,intracellular pH,and influenza virus protein acid(PA) polymerase subunit were detected with confocal microscopy and real-time fluorescent quantitative polymerase chain reaction.RESULTS:Jinchai significantly reduced the expression of HA and PA polymerase subunit mRNA in infected MDCK cells.Jinchai also significantly decreased intracellular pH in infected cells.CONCLUSIONS:Jinchai had strong anti-influenza activity against the influenza virus.It weakened the ability of the influenza virus to adsorb to cell wall and fuse with cell membranes in the early infection stage,and inhibited the transcription and replication of the virus.

Degradation of tartrazine by peroxymonosulfate through magnetic Fe_(2)O_(3)/Mn_(2)O_(3) composites activation

In this study,Fe_(2)O_(3)/Mn_(2)O_(3) composite was synthesized by a facile two-step technique,and several methods were carried out to characterize it.Then,the decomposition experiments of tartrazine(TTZ),a kind of refractory o rganic pollutant,were conducted under various enviro nmental condition to detect the catalyst performance,such as reaction system,the dosage of catalyst,peroxymonosulfate(PMS)concentration,initial pH,different natural water substances.The results exhibited that Fe_(2)O_(3)/Mn_(2)O_(3) composite with the mole rate 2:3 had the best PMS activation performance and the removal efficiency was 97.3%within 30 min.Besides,the optimum degradation conditions of TTZ we re also discussed,that is catalyst dosage(0.6 g/L),PMS concentration(0.8 g/L)and the initial pH 11.In addition,proved by the natural water substances adding experiments,HPO_(4)^(2)-,HCO_(3)^-,NO_(3)^-and NOM(nature organic matter)could slow down the experiments progressing,but Cl^-could boost it.Then inhibitor experiments indicated both the HO^·and SO_(4)^·-played a vital role in the experiments.Reusability and ions leaching experiments as well as the used catalyst physical characte rization images exhibited the excellent stability and cyclicity of the Fe_(2)O_(3)/Mn_(2)O_(3) composite.Finally,based on the XPS(X-ray photoelectro n spectro scopy)and the experiments results,the possible mechanism of TTZ degradation was proposed.This system might provide a novel thought for the decomposition of refractory organic pollutant and had potential in promotion of actual sewage treatment technology.

CoFe_(2)O_(4)nanoparticles anchored on waste eggshell for catalytic oxidation of florfenicol via activating peroxymonosulfate

Eggshell-loaded CoFe_(2)O_(4) catalyst was synthesized via a convenient hydrothermal method during our work,then the surface morphology and elemental composition of the composites were systematically investigated.Performance of CoFe_(2)O_(4)/eggshell-activated peroxymonosulfate(PMS)system was evaluated by selecting florfenicol(FF)as the model pollutant,and effects of operating parameters and water matrices on the FF removal efficiency in this system were investigated.In addition,main radicals involved in FF degradation were identified by EPR tests and radical quenching experiments,and possible mechanism was proposed.The reduction of toxicity during FF degradation was confirmed,and in combination with HP-LC tests,it was found that dehalogenation and defluorination were effectively carried out during FF degradation.In addition,the prepared CoFe_(2)O_(4)polyvinylidene fluoride(PVDF)membrane effectively improved the stability of the material and reduced the precipitation of metals.