The content of type I collagen(COL-I)and type III collagen(COL-III)and the ratio between them not only affect the skin elasticity and mechanical strength,but also determine the fibril diameter.In this research,we inve...The content of type I collagen(COL-I)and type III collagen(COL-III)and the ratio between them not only affect the skin elasticity and mechanical strength,but also determine the fibril diameter.In this research,we investigated the age-related changes in COL-I/COL-III ratio with their formed fibril diameter.The experimental result was obtained from high performance liquid chromatography-mass spectrometer,hydroxyproline determination,picrosirius red staining and transmission electron microscopes(TEM),respectively.The result indicated that the COL-I/COL-III ratio in mouse skin increased with aging.From the 0th to 9th week,the COL-I/COLIII ratio increased from 1.3:1 to 4.5:1.From the 9th to the 18th week,it remained between 4.5:1 and 4.9:1.The total content of COL-I and COL-III firstly increased and then decreased with aging.The TEM result showed that the fibril diameter increased with aging.From the 0th to 9th week,the average fibril diameter increased from 40 to 112 nm;From the 9th to 18th weeks,it increased from 112 to 140 nm.After the 9th week.The fibril diameter showed obvious uneven distribution.Thus,the COL-I/COLIII ratio was proportional to the fibril diameter,but inversely proportional to the uniformity of fibril diameter.展开更多
Acellular matrix(ACM)has been widely used as a biomaterial.As the main component of ACM,collagen type and content show influence on the material properties.In this research,the collagen in ACM from different tissues o...Acellular matrix(ACM)has been widely used as a biomaterial.As the main component of ACM,collagen type and content show influence on the material properties.In this research,the collagen in ACM from different tissues of pig were determined by detection of marker peptides.The marker peptides of Type Ⅰ and Ⅲ collagen were identified from the digested collagen standards using ions trap mass spectrometry(LCQ).The relationship between the abundance of marker peptide and collagen concentration was established using triple quadrupole mass spectrometer(TSQ).The contents of Type Ⅰ and Ⅲ collagen in ACM from different tissues were determined.The method was further verified by hydroxyproline determination.The results showed that,the sum of Type Ⅰ and Ⅲ collagen contents in the ACM from small intestinal submucosa,dermis and Achilles tendon of pig were about 87.59,81.41 and 61.13%,respectively,which were close to the total collagen contents in these tissues.The results proved that this method could quantitatively detect the collagen with different types in the ACM of various tissues.展开更多
基金supported by the National Key Technology R&D Programs of China(2021YFC2400800)Science and Technology Program of Guangzhou,China(201803010086)+1 种基金Open Funding Project of the State Key Laboratory of Biochemical Engineering(2021KF-04)Independent Research Project of the State Key Laboratory of Biochemical Engineering(2021ZZ-03).
文摘The content of type I collagen(COL-I)and type III collagen(COL-III)and the ratio between them not only affect the skin elasticity and mechanical strength,but also determine the fibril diameter.In this research,we investigated the age-related changes in COL-I/COL-III ratio with their formed fibril diameter.The experimental result was obtained from high performance liquid chromatography-mass spectrometer,hydroxyproline determination,picrosirius red staining and transmission electron microscopes(TEM),respectively.The result indicated that the COL-I/COL-III ratio in mouse skin increased with aging.From the 0th to 9th week,the COL-I/COLIII ratio increased from 1.3:1 to 4.5:1.From the 9th to the 18th week,it remained between 4.5:1 and 4.9:1.The total content of COL-I and COL-III firstly increased and then decreased with aging.The TEM result showed that the fibril diameter increased with aging.From the 0th to 9th week,the average fibril diameter increased from 40 to 112 nm;From the 9th to 18th weeks,it increased from 112 to 140 nm.After the 9th week.The fibril diameter showed obvious uneven distribution.Thus,the COL-I/COLIII ratio was proportional to the fibril diameter,but inversely proportional to the uniformity of fibril diameter.
基金supported by the National Key Technology R&D Programs of China(2018YFA0108200 and 2018YFC1106400)Beijing Nova Program(Z181100006218013,XX2018008)from the Beijing Municipal Science and Technology Commission+1 种基金Key scientific research project plan of colleges and universities in Henan Province(17A530002)the Guangzhou People’s Livelihood Science and Technology Project of China(201803010086).
文摘Acellular matrix(ACM)has been widely used as a biomaterial.As the main component of ACM,collagen type and content show influence on the material properties.In this research,the collagen in ACM from different tissues of pig were determined by detection of marker peptides.The marker peptides of Type Ⅰ and Ⅲ collagen were identified from the digested collagen standards using ions trap mass spectrometry(LCQ).The relationship between the abundance of marker peptide and collagen concentration was established using triple quadrupole mass spectrometer(TSQ).The contents of Type Ⅰ and Ⅲ collagen in ACM from different tissues were determined.The method was further verified by hydroxyproline determination.The results showed that,the sum of Type Ⅰ and Ⅲ collagen contents in the ACM from small intestinal submucosa,dermis and Achilles tendon of pig were about 87.59,81.41 and 61.13%,respectively,which were close to the total collagen contents in these tissues.The results proved that this method could quantitatively detect the collagen with different types in the ACM of various tissues.
