We previously constructed a herpes simplex virus 1(HSV-1) UL7 mutant virus(M1) and showed that a partial deletion mutation of the UL7 gene led to a lower proliferative rate and an attenuated phenotype. Using the M1 mu...We previously constructed a herpes simplex virus 1(HSV-1) UL7 mutant virus(M1) and showed that a partial deletion mutation of the UL7 gene led to a lower proliferative rate and an attenuated phenotype. Using the M1 mutant, we further modified the UL41 gene, which encodes another tegument protein, and the latency-associated transcript(LAT) gene. Observations of the resulting mutants with modified UL7 and UL41(M2) or UL7, UL41 and LAT(M3) genes indicated attenuated phenotypes, with lower proliferative ratios in various cells, non-lethal infections in mice and lower viral loads in nervous tissues compared with the wild-type strain. Furthermore, no LAT stable intron could be detected in the trigeminal ganglion of M3-infected animals. The results obtained with the three HSV-1 mutants indicate that the M3 mutant is an attenuated strain with low pathogenicity during both acute and latent infections. Together, the results support the use of the M3 mutant as a candidate for the development of an HSV-1 vaccine.展开更多
Correction to:VIROLOGICA SINICA 2017,32(5):404-414 https://doi.org/10.1007/s12250-017-3947-1 The original version of this article,published online on September 29,2017,contained errors in Fig.4B.The correct Fig.4 is g...Correction to:VIROLOGICA SINICA 2017,32(5):404-414 https://doi.org/10.1007/s12250-017-3947-1 The original version of this article,published online on September 29,2017,contained errors in Fig.4B.The correct Fig.4 is given below.展开更多
基金supported by the National Basic Research Program (2012CB518901)Chinese academy of medical sciences (CAMS) Initiative for Innovative Medicine (2016I2M-1-019)+1 种基金the National Natural Science Foundation of China (31300143, 31100127)the Fundamental Research Funds for the Central Universities (2016ZX310047, 2016ZX350072)
文摘We previously constructed a herpes simplex virus 1(HSV-1) UL7 mutant virus(M1) and showed that a partial deletion mutation of the UL7 gene led to a lower proliferative rate and an attenuated phenotype. Using the M1 mutant, we further modified the UL41 gene, which encodes another tegument protein, and the latency-associated transcript(LAT) gene. Observations of the resulting mutants with modified UL7 and UL41(M2) or UL7, UL41 and LAT(M3) genes indicated attenuated phenotypes, with lower proliferative ratios in various cells, non-lethal infections in mice and lower viral loads in nervous tissues compared with the wild-type strain. Furthermore, no LAT stable intron could be detected in the trigeminal ganglion of M3-infected animals. The results obtained with the three HSV-1 mutants indicate that the M3 mutant is an attenuated strain with low pathogenicity during both acute and latent infections. Together, the results support the use of the M3 mutant as a candidate for the development of an HSV-1 vaccine.
文摘Correction to:VIROLOGICA SINICA 2017,32(5):404-414 https://doi.org/10.1007/s12250-017-3947-1 The original version of this article,published online on September 29,2017,contained errors in Fig.4B.The correct Fig.4 is given below.