The truncated form of fagellin (tFlic) provides the 2dCap subunit vaccine with better immunogenicity and protective efects in mice

Porcine circovirus type 2(PCV2)is the main causative agent of porcine circovirus-associated diseases,and it causes substantial economic losses in the swine industry each year.It is crucial to develop an efective vaccine against the circulating strain PCV2d,which is prone to substantial degrees of mutation.In this study,a truncated form of fagellin(tFlic:85-111 aa)was inserted into the C-terminal sequence of 2dCap,and Western blotting results showed that recombinant Cap-tFlic VLPs were successfully expressed.Transmission electron microscopy(TEM)and dynamic light scattering(DLS)data indicated that purifed recombinant Cap-tFlic fusion proteins existed in the form of polymers and that tFlic could not afect the formation and internalization of VLPs.Integrated Cap-tFlic VLPs induced the expression of antigen presentation-related factors(MHC-II and CD86)by bone marrow-derived dendritic cells(BM-DCs),and the expression of TLR5-related factors(TNF-α)was dramatically elevated.Mice intramuscularly immunized with Cap-tFlic VLPs exhibited signifcantly higher levels of Cap-specifc antibodies and neutralizing antibodies than mice immunized with wild-type Cap VLPs.The data obtained in the current study indicate that Cap-tFlic may be a candidate for a subunit vaccine against PCV2 in the future.

应急治理中的合作生产何以有效?——以中国灾害群测群防体系的实践为例

在利益诉求多元的应急治理实践中,公共应急产品的合作生产是应对“政府失灵”与“市场失灵”困境的有效手段,如何提升应急治理中合作生产的有效性已成为增强防灾减灾效能的前沿议题。研究以合作生产理论为基础,从主体能力与主体间关系的整体性视角出发,采用多元回归分析法对四川省汶川县899个家庭户的调研数据展开实证分析,以此识别影响应急治理中合作生产有效性的因素并阐明其内在机理。研究结果表明,主体能力维度中的领导力、学习能力以及主体间关系维度中的信任、凝聚力等要素均对公共应急服务的合作生产有效性产生显著的正向影响。同时,信任作为调节变量,分别强化了凝聚力与领导力对合作生产有效性的正向作用。由此可见,要想实现持续有效的应急公共产品供给,不仅应关注生产主体的能力建设,同时也要重视主体间的互动模式与关系网络,因地制宜地实现应急服务的优化供给。