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基于细胞自噬探讨缺氧条件下黄芪多糖促进血管新生的机制 被引量:1
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作者 周严东 高娜 +6 位作者 陈叶飞 孙英新 张瑞峰 郑宇佳 李雅文 杨琳 姜希娟 《阿尔茨海默病及相关病杂志》 2023年第1期35-42,共8页
目的:探究黄芪多糖(astragalus polysaccharide,APS)在缺氧条件下促进血管新生的作用机制与靶点。方法:于体外鼠尾胶基质上种植胸主动脉血管段构建新生血管三维培养模型,随机分为对照组、APS25 mg·L^(-1)组,利用倒置显微镜观察新... 目的:探究黄芪多糖(astragalus polysaccharide,APS)在缺氧条件下促进血管新生的作用机制与靶点。方法:于体外鼠尾胶基质上种植胸主动脉血管段构建新生血管三维培养模型,随机分为对照组、APS25 mg·L^(-1)组,利用倒置显微镜观察新生血管生长情况。体外培养大鼠脑微血管内皮细胞,以氯化钴400μmol·L^(-1)诱导24 h,形成可复制的缺氧模型,随机分为7组(n=6):对照组、缺氧模型组、APS25 mg·L^(-1)组、自噬激动剂雷帕霉素10 mg·L^(-1)组,阳性对照药巴弗洛霉素组20mg·L-1,APS加雷帕霉素组,APS加巴弗洛霉素组。然后利用CCK-8比色法检测各组细胞的增殖活性;蛋白印迹法检测各组细胞自噬相关蛋白Becline-1、LC3B、P62的表达。结果:(1)血管新生实验:与对照组相比,经APS干预新生血管数量明显增多(P<0.05)。(2)细胞实验:与模型组相比,经APS治疗内皮细胞增殖能力提高(P<0.05),Becline-1、LC3B蛋白表达量下调(P<0.01)。结论:在缺氧条件下,黄芪多糖可通过促进血管内皮细胞增殖,促进血管新生,其机制可能与下调内皮细胞Becline-1、LC3B的表达以抑制自噬发生有关。 展开更多
关键词 血管新生 脑微血管内皮细胞 细胞自噬 细胞迁移 黄芪多糖 缺血性脑卒中
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Ghost-Retina Net:Fast Shadow Detection Method for Photovoltaic Panels Based on Improved Retina Net 被引量:1
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作者 Jun Wu Penghui Fan +1 位作者 yingxin sun Weifeng Gui 《Computer Modeling in Engineering & Sciences》 SCIE EI 2023年第2期1305-1321,共17页
Based on the artificial intelligence algorithm of RetinaNet,we propose the Ghost-RetinaNet in this paper,a fast shadow detection method for photovoltaic panels,to solve the problems of extreme target density,large ove... Based on the artificial intelligence algorithm of RetinaNet,we propose the Ghost-RetinaNet in this paper,a fast shadow detection method for photovoltaic panels,to solve the problems of extreme target density,large overlap,high cost and poor real-time performance in photovoltaic panel shadow detection.Firstly,the Ghost CSP module based on Cross Stage Partial(CSP)is adopted in feature extraction network to improve the accuracy and detection speed.Based on extracted features,recursive feature fusion structure ismentioned to enhance the feature information of all objects.We introduce the SiLU activation function and CIoU Loss to increase the learning and generalization ability of the network and improve the positioning accuracy of the bounding box regression,respectively.Finally,in order to achieve fast detection,the Ghost strategy is chosen to lighten the size of the algorithm.The results of the experiment show that the average detection accuracy(mAP)of the algorithm can reach up to 97.17%,the model size is only 8.75 MB and the detection speed is highly up to 50.8 Frame per second(FPS),which can meet the requirements of real-time detection speed and accuracy of photovoltaic panels in the practical environment.The realization of the algorithm also provides new research methods and ideas for fault detection in the photovoltaic power generation system. 展开更多
关键词 Deep learning intensive object detection photovoltaic panel shadow Ghost module retinanet
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Application of Blended Learning Model to Computer Fundamentals Courses in an Online Environment
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作者 Xin Lv yingxin sun Gang Chen 《Journal of Contemporary Educational Research》 2020年第7期63-67,共5页
In the online environment,schools should keep up with the pace of development and strengthen the application of hybrid teaching mode,so as to strengthen the cultivation of students’comprehensive computer application ... In the online environment,schools should keep up with the pace of development and strengthen the application of hybrid teaching mode,so as to strengthen the cultivation of students’comprehensive computer application ability and computer subject literacy,and improve students’learning efficiency.The author explores the actual situation of teaching basic computer courses at this stage and the requirements of applying hybrid teaching mode to carry out computer basic course teaching under the network environment,and puts forward an effective strategy for the application of hybrid teaching mode in computer basic course under the network environment,hoping to contribute to the improvement of the teaching quality and quality of computer basic course. 展开更多
关键词 Online environment Blended learning Computer-based courses
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Raman signal enhancement for gas detection using a dual near-concentric cavities group
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作者 任一凡 杨德旺 +3 位作者 孙颖欣 许家旋 刘硕芳 陈月娥 《Chinese Optics Letters》 SCIE EI CAS CSCD 2024年第5期47-52,共6页
Effective methods are urgently required to optimize Raman spectroscopy technology to ameliorate its low detection sensitivity.Here,we superposed two near-concentric cavities to develop a dual near-concentric cavities ... Effective methods are urgently required to optimize Raman spectroscopy technology to ameliorate its low detection sensitivity.Here,we superposed two near-concentric cavities to develop a dual near-concentric cavities group(DNCCG)to assess its effect on gas Raman signal intensity,signal-to-noise ratio(SNR),and limit of detection(LOD).The results showed that DNCCG generally had higher CO_(2) Raman signal intensity than the sum of two near-concentric cavities.Meanwhile,the noise intensity of DNCCG was not enhanced by the superposition of near-concentric cavities.Accordingly,DNCCG increased the SNR.The LOD for CO_(2) was 24.6 parts per million.DNCCG could be an effective method to improve the detection capability of trace gases and broaden the dynamic detection range,which might aid the future development of innovative technology for multicomponent gas detection. 展开更多
关键词 Raman spectroscopy gas detection sensitivity dual near-concentric cavities group
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