DNA nanotechnology has been widely employed for biomedical applications.However,most DNA nanomaterials rely on noncovalent complementary base pairing of short single-stranded DNA oligonucleotides.Herein,we describe a ...DNA nanotechnology has been widely employed for biomedical applications.However,most DNA nanomaterials rely on noncovalent complementary base pairing of short single-stranded DNA oligonucleotides.Herein,we describe a general strategy to construct a long and covalently conjugated branched DNA structure for fast and in situ gelation in vivo.In our design,a short and covalently conjugated branched DNA structure can normally be employed as the DNA primer in the terminal deoxynucleotidyl transferase-dependent enzymatic polymerization system.After enzymatic extension,the DNA aptamer-modified branched DNA structures with the sequences of poly T or poly A can immediately coassemble for in situ encapsulation of the target protein and tumor cell.The fast and in situ gelation system can function in a murine model of local tumor recurrence for targeting residual tumor cells to achieve long-term drug release for efficient tumor inhibition in vivo.This rationally developed DNA self-assembly strategy provides a new avenue for the development of multifunctional DNA nanomaterials.展开更多
Nucleic acids are natural macromolecules with the ability to store and transmit information based on the strict base-pairing principle.Beyond the natural nucleic acid double helixes,various DNA/RNA nanostructures with...Nucleic acids are natural macromolecules with the ability to store and transmit information based on the strict base-pairing principle.Beyond the natural nucleic acid double helixes,various DNA/RNA nanostructures with customized geometries and functionalities have been fabricated.Featured with programmability and sequence-dependent responsiveness,DNA/RNA nanostructures have been employed for the rational design and construction of logic devices.When stimulated by internal molecular triggers and/or external stimuli,these logic gate devices can operate at nanoscale level in complex biological environments,performing logic operations and producing corresponding outputs.In this minireview,we summarize the recent advances of nucleic acid logic devices,which are responsive to various stimuli,including DNA/RNA strands,metal ions,small molecules,peptides,proteins,photo-irradiation,pH changes,and so forth.The applications of these devices in biosensing and biofunction regulation are also included.In the last part of the present study,we discuss the remaining challenges and perspectives of nucleic acid logic devices.展开更多
基金the National Key R&D Program of China(grant nos.2021YFA1200302 and 2018YFA0208900)the National Natural Science Foundation of China(grant nos.22025201,22077023,and 21721002)+2 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(grant no.XDB36000000)the CAS Project for Young Scientists in Basic Research(grant no.YSBR-036)CAS Interdisciplinary Innovation Team,the Youth Innovation Promotion Association CAS,and the K.C.Wong Education Foundation(grant no.GJTD-2018-03).
文摘DNA nanotechnology has been widely employed for biomedical applications.However,most DNA nanomaterials rely on noncovalent complementary base pairing of short single-stranded DNA oligonucleotides.Herein,we describe a general strategy to construct a long and covalently conjugated branched DNA structure for fast and in situ gelation in vivo.In our design,a short and covalently conjugated branched DNA structure can normally be employed as the DNA primer in the terminal deoxynucleotidyl transferase-dependent enzymatic polymerization system.After enzymatic extension,the DNA aptamer-modified branched DNA structures with the sequences of poly T or poly A can immediately coassemble for in situ encapsulation of the target protein and tumor cell.The fast and in situ gelation system can function in a murine model of local tumor recurrence for targeting residual tumor cells to achieve long-term drug release for efficient tumor inhibition in vivo.This rationally developed DNA self-assembly strategy provides a new avenue for the development of multifunctional DNA nanomaterials.
基金National Natural Science Foundation of China,Grant/Award Numbers:22025201,21721002,32071389,21773044,51761145044K.C.Wong Education Foundation,Grant/Award Number:GJTD-2018-03+2 种基金Youth Innovation Promotion Association,CAS,Grant/Award Number:QYZDBSSW-SLH029Beijing Municipal Science&Technology Commission,Grant/Award Number:Z191100004819008National Basic Research Program of China,Grant/Award Numbers:2018YFA0208900,2016YFA0201601。
文摘Nucleic acids are natural macromolecules with the ability to store and transmit information based on the strict base-pairing principle.Beyond the natural nucleic acid double helixes,various DNA/RNA nanostructures with customized geometries and functionalities have been fabricated.Featured with programmability and sequence-dependent responsiveness,DNA/RNA nanostructures have been employed for the rational design and construction of logic devices.When stimulated by internal molecular triggers and/or external stimuli,these logic gate devices can operate at nanoscale level in complex biological environments,performing logic operations and producing corresponding outputs.In this minireview,we summarize the recent advances of nucleic acid logic devices,which are responsive to various stimuli,including DNA/RNA strands,metal ions,small molecules,peptides,proteins,photo-irradiation,pH changes,and so forth.The applications of these devices in biosensing and biofunction regulation are also included.In the last part of the present study,we discuss the remaining challenges and perspectives of nucleic acid logic devices.
