Spatial distribution characteristics of bacterial community structure and gene abundance in sediments of the Bohai Sea

This study investigated differences in the community structure and environmental responses of the bacterial community in sediments of the Bohai Sea.Illumina high-throughput sequencing technology and real-time PCR were used to assay the bacterial 16S rRNA genes in the surface sediments of 13 sampling stations in the Bohai Sea.The results showed that sediments at the majority of the 13 sampling stations were contaminated by heavy metal mercury.The main phyla of bacteria recorded included Proteobacteria(52.92%),Bacteroidetes(11.76%),Planctomycetes(7.39%),Acidobacteria(6.53%)and Chloroflexi(4.97%).The genus with the highest relative abundance was Desulfobulbus(4.99%),which was the dominant genus at most sampling stations,followed by Lutimonas and Halioglobus.The main factors influencing bacterial community structure were total organic carbon,followed by depth and total phosphorus.The content of lead,cadmium,chromium,copper and zinc had a consistent effect on community structure.Arsenic showed a negative correlation with bacterial community structure in most samples,while the impact of mercury on community structure was not significant.The bacterial community in sediment samples from the Bohai Sea was rich in diversity and displayed an increase in diversity from high to low latitudes.The data indicated that the Bohai Sea had abundant microbial resources and was rich in bacteria with the potential to metabolize many types of pollutants.

Evaluation of the Safety of Mine Road Based on Fuzzy Analytic Hierarchy Process

Generally, mine roads are located in the mountain areas, as its complex topography, mostly along the river near the cliffs, steep bend anxious, the mine road design has to adopt lower technical standards relatively and usually is lack of traffic safety facilities. Especially, there are mainly medium-sized vehicles on mine road, under the heavy traffic vehicles affect repeatedly, high frequency of traffic accidents more easily happen in mine road area and cause serious effects on life or property. Combining with the particularity of mine road safety environment, this paper studies the basic theory of safety evaluation, analyses the factors of traffic safety design and special mine terrain conditions, and then establishes mine road safety index system and evaluation model based on the principles such as systematicness, independent indexes, qualitative and quantitative analysis, feasibility, scientificity and reliability. At last, the paper successfully evaluates the safety of road in Huang Mailing phosphate rock area with fuzzy AHP method based on engineering project.

A Review of Speech Rhythm Pattern in Second Language Acquisition

As an important prosodic feature in the processing of natural languages,speech rhythm refers to the temporal organization of language.The present study reviewed the literature related to speech rhythm pattern of second language.It is indicated most of the previous studies only focused on the ESL learners’speech rhythm patterns.However,there are a few studies on the rhythm patterns of Mandarin Chinese learners.Thus,it is necessary to investigate the rhythmic patterns of different second language learners because rhythmic cues are important to evaluate the degree of accentedness in speech.