Objective： The aim of this study was to observe the efficacy of air wave pressure therapeutic equipment in pre- vention of oxaliplatin-inducted neurotoxicity. Methods： Forty-five patients with colorectal cancer were...Objective： The aim of this study was to observe the efficacy of air wave pressure therapeutic equipment in pre- vention of oxaliplatin-inducted neurotoxicity. Methods： Forty-five patients with colorectal cancer were randomly divided into treatment group and control group, treatment group were given the treatment of air wave pressure therapeutic equipment during chemotherapy with oxaliplatin, the control group were given preventive treatment, the oxaliplatin-inducted neurotoxicity was evaluated after each cycle of chemotherapy. Evaluate the chemotherapy efficacy after the third cycle and sixth cycle of chemotherapy. Results： The treatment group have lower incidence of peripheral nerve toxicity than the control group, the difference was statistically significant （X2= 13.93; P 〈 0.01）. Chemotherapy effect between the 2 groups was no significant difference （P 〉 0.05）. Conclusion： Treatment with air wave pressure therapeutic equipment can reduce the incidence of peripheral nerve toxicity during oxaliplatin chemotherapy.展开更多
Objective:To establish a new genomic signature for the prognosis of survival in relation to the tumor microenvironment in esophageal adenocarcinoma.Methods:Data from The Cancer Genome Atlas(TCGA)were applied,and the s...Objective:To establish a new genomic signature for the prognosis of survival in relation to the tumor microenvironment in esophageal adenocarcinoma.Methods:Data from The Cancer Genome Atlas(TCGA)were applied,and the stromal and immune scores of patients with esophageal adenocarcinoma(EAC)were generated through the ESTIMATE algorithm.Differentially expressed genes were obtained,and genes concerning immune prognosis were identified on the basis of these scores.Functional analysis showed that these genes were primarily involved in immunobiological processes.Additionally,CIBERSORT was used to analyze 22 subgroups of tumor-infiltrating immune cells in the tumor microenvironment.Results:The results of the genomic assessment shown on the Kaplan-Meier curve revealed that EAC patients with high-risk scores have the worst survival.The risk score is valid as an independent prognostic factor for the overall survival in EAC patients.The tumor microenvironment was systematically analyzed,and the immune-related prognostic biomarkers of EAC have been proposed.Conclusion:The expression of tumor-infiltrating immune cells and immune-related genes in EAC have been identified.Some previously overlooked genes may be used as additional biomarkers for EAC in the future.展开更多
Background:The lens zonule,a circumferential system of fibres connecting the ciliary body to the lens,is responsible for centration of the lens.The structural,functional,and positional abnormalities of the zonular app...Background:The lens zonule,a circumferential system of fibres connecting the ciliary body to the lens,is responsible for centration of the lens.The structural,functional,and positional abnormalities of the zonular apparatus can lead to the abnormality of the intraocular structure,presenting a significant challenge to cataract surgery.Main text:The lens zonule is the elaborate system of extracellular fibers,which not only centers the lens in the eye but also plays an important role in accommodation and lens immunity,maintains the shape of the lens,and corrects spherical aberration.The zonules may directly participate in the formation of cataract via the immune mechanism.Abnormal zonular fibers that affect the position and shape of the lens may play an important role in the pathogenesis of angle closure disease and increase the complexity of the surgery.Capsular tension rings and related endocapsular devices are used to provide sufficient capsular bag stabilization and ensure the safety of cataract surgery procedures.Better preoperative and intraoperative evaluation methods for zonules are needed for clinicians.Conclusions:The microstructure,biomechanical properties,and physiological functions of the lens zonules help us to better understand the pathogenesis of cataract and glaucoma,facilitating the development of safer surgical procedures for cataract.Further studies are needed to carefully analyze the structure–function relationship of the zonular apparatus to explore new treatment strategies for cataract and glaucoma.展开更多
Background:Pressure ulcers(PUs)are a major clinical problem that constitutes a tremendous economic burden on healthcare systems.Deep tissue injury(DTI)is a unique serious type of pressure ulcer that arises in skeletal...Background:Pressure ulcers(PUs)are a major clinical problem that constitutes a tremendous economic burden on healthcare systems.Deep tissue injury(DTI)is a unique serious type of pressure ulcer that arises in skeletal muscle tissue.DTI arises in part because skeletal muscle tissues are more susceptible than skin to external compression.Unfortunately,few effective therapies are currently available for muscle injury.Basic fibroblast growth factor(bFGF),a potent mitogen and survival factor for various cells,plays a crucial role in the regulation of muscle development and homeostasis.The main purpose of this study was to test whether local administration of bFGF could accelerate muscle regeneration in a rat DTI model.Methods:Male Sprague Dawley(SD)rats(age 12 weeks)were individually housed in plastic cages and a DTI PU model was induced according to methods described before.Animals were randomly divided into three groups:a normal group,a PU group treated with saline,and a PU group treated with bFGF(10μg/0.1 ml)subcutaneously near the wound.Results:We found that application of bFGF accelerated the rate of wound closure and promoted cell proliferation and tissue angiogenesis.In addition,compared to saline administration,bFGF treatment prevented collagen deposition,a measure of fibrosis,and up-regulated the myogenic marker proteins MyHC and myogenin,suggesting bFGF promoted injured muscle regeneration.Moreover,bFGF treatment increased levels of myogenesis-related proteins p-Akt and p-mTOR.Conclusions:Our findings show that bFGF accelerated injured skeletal muscle regeneration through activation of the PI3K/Akt/mTOR signaling pathway and suggest that administration of bFGF is a potential therapeutic strategy for the treatment of skeletal muscle injury in PUs.展开更多
文摘Objective： The aim of this study was to observe the efficacy of air wave pressure therapeutic equipment in pre- vention of oxaliplatin-inducted neurotoxicity. Methods： Forty-five patients with colorectal cancer were randomly divided into treatment group and control group, treatment group were given the treatment of air wave pressure therapeutic equipment during chemotherapy with oxaliplatin, the control group were given preventive treatment, the oxaliplatin-inducted neurotoxicity was evaluated after each cycle of chemotherapy. Evaluate the chemotherapy efficacy after the third cycle and sixth cycle of chemotherapy. Results： The treatment group have lower incidence of peripheral nerve toxicity than the control group, the difference was statistically significant （X2= 13.93; P 〈 0.01）. Chemotherapy effect between the 2 groups was no significant difference （P 〉 0.05）. Conclusion： Treatment with air wave pressure therapeutic equipment can reduce the incidence of peripheral nerve toxicity during oxaliplatin chemotherapy.
基金College-Level Youth Fund Project(Project Number:ZZYQ2012).
文摘Objective:To establish a new genomic signature for the prognosis of survival in relation to the tumor microenvironment in esophageal adenocarcinoma.Methods:Data from The Cancer Genome Atlas(TCGA)were applied,and the stromal and immune scores of patients with esophageal adenocarcinoma(EAC)were generated through the ESTIMATE algorithm.Differentially expressed genes were obtained,and genes concerning immune prognosis were identified on the basis of these scores.Functional analysis showed that these genes were primarily involved in immunobiological processes.Additionally,CIBERSORT was used to analyze 22 subgroups of tumor-infiltrating immune cells in the tumor microenvironment.Results:The results of the genomic assessment shown on the Kaplan-Meier curve revealed that EAC patients with high-risk scores have the worst survival.The risk score is valid as an independent prognostic factor for the overall survival in EAC patients.The tumor microenvironment was systematically analyzed,and the immune-related prognostic biomarkers of EAC have been proposed.Conclusion:The expression of tumor-infiltrating immune cells and immune-related genes in EAC have been identified.Some previously overlooked genes may be used as additional biomarkers for EAC in the future.
文摘Background:The lens zonule,a circumferential system of fibres connecting the ciliary body to the lens,is responsible for centration of the lens.The structural,functional,and positional abnormalities of the zonular apparatus can lead to the abnormality of the intraocular structure,presenting a significant challenge to cataract surgery.Main text:The lens zonule is the elaborate system of extracellular fibers,which not only centers the lens in the eye but also plays an important role in accommodation and lens immunity,maintains the shape of the lens,and corrects spherical aberration.The zonules may directly participate in the formation of cataract via the immune mechanism.Abnormal zonular fibers that affect the position and shape of the lens may play an important role in the pathogenesis of angle closure disease and increase the complexity of the surgery.Capsular tension rings and related endocapsular devices are used to provide sufficient capsular bag stabilization and ensure the safety of cataract surgery procedures.Better preoperative and intraoperative evaluation methods for zonules are needed for clinicians.Conclusions:The microstructure,biomechanical properties,and physiological functions of the lens zonules help us to better understand the pathogenesis of cataract and glaucoma,facilitating the development of safer surgical procedures for cataract.Further studies are needed to carefully analyze the structure–function relationship of the zonular apparatus to explore new treatment strategies for cataract and glaucoma.
基金This study was supported by research grants from the Zhejiang Provincial Natural Science Funding(LY14H150008)the National Natural Science Funding of China(81372064,81472165,and 81572237)+2 种基金the Zhejiang Provincial Program of Medical and Health Science(2014KYA131)the Wenzhou Program of Science and Technology(Y20140003)the State Key Basic Research Development Program(2012CB518105)
文摘Background:Pressure ulcers(PUs)are a major clinical problem that constitutes a tremendous economic burden on healthcare systems.Deep tissue injury(DTI)is a unique serious type of pressure ulcer that arises in skeletal muscle tissue.DTI arises in part because skeletal muscle tissues are more susceptible than skin to external compression.Unfortunately,few effective therapies are currently available for muscle injury.Basic fibroblast growth factor(bFGF),a potent mitogen and survival factor for various cells,plays a crucial role in the regulation of muscle development and homeostasis.The main purpose of this study was to test whether local administration of bFGF could accelerate muscle regeneration in a rat DTI model.Methods:Male Sprague Dawley(SD)rats(age 12 weeks)were individually housed in plastic cages and a DTI PU model was induced according to methods described before.Animals were randomly divided into three groups:a normal group,a PU group treated with saline,and a PU group treated with bFGF(10μg/0.1 ml)subcutaneously near the wound.Results:We found that application of bFGF accelerated the rate of wound closure and promoted cell proliferation and tissue angiogenesis.In addition,compared to saline administration,bFGF treatment prevented collagen deposition,a measure of fibrosis,and up-regulated the myogenic marker proteins MyHC and myogenin,suggesting bFGF promoted injured muscle regeneration.Moreover,bFGF treatment increased levels of myogenesis-related proteins p-Akt and p-mTOR.Conclusions:Our findings show that bFGF accelerated injured skeletal muscle regeneration through activation of the PI3K/Akt/mTOR signaling pathway and suggest that administration of bFGF is a potential therapeutic strategy for the treatment of skeletal muscle injury in PUs.
