Organic solar cells(OSCs) especially non-fullerene OSCs(NF-OSCs) are promising to become the next-generation of commercial applications and have received great attention from many researchers due to their typical adva...Organic solar cells(OSCs) especially non-fullerene OSCs(NF-OSCs) are promising to become the next-generation of commercial applications and have received great attention from many researchers due to their typical advantages of low cost,light weight,and flexibility [1,2].展开更多
The quantitative realtime polymerase chain reaction(RT-qPCR)is a powerful and sensitive method to measure expression of targeted gene but it highly relies on the use of suitable reference genes for data normalization....The quantitative realtime polymerase chain reaction(RT-qPCR)is a powerful and sensitive method to measure expression of targeted gene but it highly relies on the use of suitable reference genes for data normalization.We evaluated the expressions of 8 housekeeping genes:18S ribosomal rDNA(18S rDNA),28S ribosomal r DNA(28S rDNA),rubisco large subunit(rbc-L),β-actin(ACT),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),elongation factor 1(EF1),β-tubulin(Tub B),and P-phycoerythrin B(PEB),to select the suitable reference genes for different life-history stages(tetrasporophyte,carposporophyte,and male/female gametophyte)of Gracilaria vermiculophylla by absolute quantitative method.Softwares geNorm and BestKeeper were used to verify the results acquired from copy number analysis.Results show that the expression of identified reference genes varied in comparing groups composed of different type of life stages.It is suggested that 18S rDNA and TubB could be used for highly complex samples composed of mixed ploidy and phases.18S rDNA and 28S rDNA were also preferred for using among the matured isomorphic samples.But for samples with different maturities,TubB and ACT were recommended for tetrasporophytes and gametophytes respectively.展开更多
A compact surface plasmon resonance(SPR) fiber optic sensor, being utilized to simultaneously measure refractive index(RI) and temperature, is proposed and experimentally demonstrated in this paper. One part of a no-c...A compact surface plasmon resonance(SPR) fiber optic sensor, being utilized to simultaneously measure refractive index(RI) and temperature, is proposed and experimentally demonstrated in this paper. One part of a no-core fiber(NCF)was coated with a silver(Ag) film, and the other part was coated with a silver/polydimethylsiloxane(Ag/PDMS) composite film to stimulate the SPR effect. Due to the two heterogeneous films, two dips appeared in the transmission spectrum and were used to achieve the dual-parameter measurements. The experimental results showed that the RI sensitivity reached 2121.43 nm/RIU and 0 nm/RIU, while the temperature sensitivity reached-0.32 nm/℃ and-2.21 nm/℃ for the two dips,respectively. Based on the obtained transfer matrix, the measurements of RI and temperature could be demodulated. This designed sensor showed the merits of simple structure, easy to implement, and high sensitivity, demonstrating application prospects in dual-parameter monitoring.展开更多
基金the Natural Science Foundation of China (52172048, 52103221, 51873172, 22205130, 12175298)the Shandon Provincial Natural Science Foundation of China (ZR2021QB024, ZR2021QB179, ZR2021ZD06)+2 种基金the Guangdong Natural Science Foundation of China (2023A1515012323, 2023A1515010943, 2022A1515110643)the National Key Research and Development Program of China (2022YFB4200400) funded by MOST of Chinathe Fundamental Research Funds of Shandong University from China。
文摘Organic solar cells(OSCs) especially non-fullerene OSCs(NF-OSCs) are promising to become the next-generation of commercial applications and have received great attention from many researchers due to their typical advantages of low cost,light weight,and flexibility [1,2].
基金Supported by the Key Program of Science and Technology Innovation in Ningbo (No.2019B10009)the National Natural Science Foundation of China (No.41476111)。
文摘The quantitative realtime polymerase chain reaction(RT-qPCR)is a powerful and sensitive method to measure expression of targeted gene but it highly relies on the use of suitable reference genes for data normalization.We evaluated the expressions of 8 housekeeping genes:18S ribosomal rDNA(18S rDNA),28S ribosomal r DNA(28S rDNA),rubisco large subunit(rbc-L),β-actin(ACT),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),elongation factor 1(EF1),β-tubulin(Tub B),and P-phycoerythrin B(PEB),to select the suitable reference genes for different life-history stages(tetrasporophyte,carposporophyte,and male/female gametophyte)of Gracilaria vermiculophylla by absolute quantitative method.Softwares geNorm and BestKeeper were used to verify the results acquired from copy number analysis.Results show that the expression of identified reference genes varied in comparing groups composed of different type of life stages.It is suggested that 18S rDNA and TubB could be used for highly complex samples composed of mixed ploidy and phases.18S rDNA and 28S rDNA were also preferred for using among the matured isomorphic samples.But for samples with different maturities,TubB and ACT were recommended for tetrasporophytes and gametophytes respectively.
基金the Natural Science Foundation of Hebei Province, China (Grant No. F2021203112)the National Natural Science Foundation of China (Grant No. 12074331)+1 种基金the National Key Research and Development Program of China (Grant No. 2019YFB2204001)Basic Scientific Research Funds for universities in Hebei Province, China (Grant No. JQN2021019)。
文摘A compact surface plasmon resonance(SPR) fiber optic sensor, being utilized to simultaneously measure refractive index(RI) and temperature, is proposed and experimentally demonstrated in this paper. One part of a no-core fiber(NCF)was coated with a silver(Ag) film, and the other part was coated with a silver/polydimethylsiloxane(Ag/PDMS) composite film to stimulate the SPR effect. Due to the two heterogeneous films, two dips appeared in the transmission spectrum and were used to achieve the dual-parameter measurements. The experimental results showed that the RI sensitivity reached 2121.43 nm/RIU and 0 nm/RIU, while the temperature sensitivity reached-0.32 nm/℃ and-2.21 nm/℃ for the two dips,respectively. Based on the obtained transfer matrix, the measurements of RI and temperature could be demodulated. This designed sensor showed the merits of simple structure, easy to implement, and high sensitivity, demonstrating application prospects in dual-parameter monitoring.
