Ultrasmall AuPd nanoclusters on amine-functionalized carbon blacks as high-performance bi-functional catalysts for ethanol electrooxidation and formic acid dehydrogenation

The synthesis of ultrasmall metal nanoclusters(NCs) with high catalytic activities is of great importance for the development of clean and renewable energy technologies but remains a challenge. Here we report a facile wet-chemical method to prepare ~1.0 nm Au Pd NCs supported on amine-functionalized carbon blacks. The Au Pd NCs exhibit a specific activity of 5.98 mA cm_(AuPd)^(-2)and mass activity of 5.25 A mg_(auPd)^(-1) for ethanol electrooxidation, which are far better than those of commercial Pd/C catalysts(1.74 mAcm_(AuPd)^(-2) and 0.54 A mg_(Pd)^(-1) ). For formic acid dehydrogenation, the Au Pd NCs have an initial turn over frequency of 49339 h^(-1) at 298 K without any additive, which is much higher than those obtained for most of reported Au Pd catalysts. The reported synthesis may represent a facile and low-cost approach to prepare other ultrasmall metal NCs with high catalytic activities for various applications.

Molecular Detection of FGFR2 Rearrangements in Resected Intrahepatic Cholangiocarcinomas:FISH Could Be An Ideal Method in Patients with Histological Small Duct Subtype

Background and Aims:Intrahepatic cholangiocarcinoma(ICC)is a subtype of primary liver cancer for which effective therapeutic agents are lacking.Fibroblast growth factor receptor 2(FGFR2)has become a promising therapeutic target in ICC;however,its incidence and optimum testing method have not been fully assessed.This study investigated the rearrangement of FGFR2 in intrahepatic cholangiocarcinoma using multiple molecular detection methods.Methods:The samples and clinical data of 167 patients who underwent surgical resection of intrahepatic cholangiocarcinoma in Zhongshan hospital,Fudan university were collected.The presence of FGFR2 gene rearrangement was confirmed using fluorescence in situ hybridization(FISH)and targeted next-generation sequencing(NGS).FGFR2 protein expression was determined using immunohistochemistry(IHC).The concordance between the methods was statistically compared.PD-L1 expression was also assessed in this cohort.The clinicopathological characteristics and genomic profile related to FGFR2 rearrangements were also analyzed to assist candidatescreening for targeted therapies.Results:FGFR2 rearrangement was detected in 21 of the 167 ICC cases(12.5%)using FISH.NGS analysis revealed that FGFR2 rearrangement was present in 16 of the 20 FISH-positive cases,which was consistent with the FISH results(kappa value=0.696,p<0.01).IHC showed that 80 of the 167 cases(48%)were positive for FGFR2 expression,which was discordant with both FISH and NGS results.By comparison,FGFR2-positivity tended to correlate with unique clinicopathological subgroups,featuring early clinical stage,histologically small duct subtype,and reduced mucus production(P<0.05),with improved overall survival(p<0.05).FGFR2-positivity was not associated with PD-L1 expression in ICCs.In genome research,we identified eight partner genes fused with FGFR2,among which FGFR2-BICC1 was the most common fusion type.BAP1,CDKN2A,and CDKN2B were the most common concomitant genetic alterations of FGFR2,whereas KRAS and IDH1 mutations were mutually exclusive to FGFR2 rearrangements.Conclusions:FISH achieved satisfactory concordance with NGS,has potential value for FGFR2 screening for targeted therapies.FGFR2 detection should be prioritized for unique clinical subgroups in ICC,which features a histological small duct subtype,early clinical stage,and reduced mucus production.