The contact problem for the elastic sphere indenting a layered half-space is considered. Analytical methods for solving this problem have been developed on the basis of the 3-D fundamental solution of a half space wit...The contact problem for the elastic sphere indenting a layered half-space is considered. Analytical methods for solving this problem have been developed on the basis of the 3-D fundamental solution of a half space with a single coating layer under a normal concentrated force on the surface. The normal pressure distribution within the contact zone is assumed as Hertzian type. The solutions are constructed using superposition principle in the form of infinite series. Through comparing with the numerical results of FEM,it can be verified that the exact solutions have a rapid convergence rate and the stresses and displacements are mainly determined by the first term,which is corresponding to the solution of homogeneous half-space under Hertzian loading. The contact radius can be predicted applying the method.展开更多
As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen...As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen presentation.To elucidate the key inhibitory factor in regulating the interaction of infected dendritic cells(DCs)and T cells,DCs were pretreated with the NDV vaccine strain LaSota as an inhibitor and stimulated with lipopolysaccharide(LPS)for further detection by enzyme-linked immunosorbent assay(ELISA),flow cytometry,immunoblotting,and quantitative real-time polymerase chain reaction(qRT-PCR).The results revealed that NDV infection resulted in the inhibition of interleukin(IL)-12p40 in DCs through a p38 mitogen-activated protein kinase(MAPK)-dependent manner,thus inhibiting the synthesis of IL-12p70,leading to the reduction in T cell proliferation and the secretion of interferon-(IFN-),tumor necrosis factor-α(TNF-α),and IL-6 induced by DCs.Consequently,downregulated cytokines accelerated the infection and viral transmission from DCs to T cells.Furthermore,several other strains of NDV also exhibited inhibitory activity.The current study reveals that NDV can modulate the intensity of the innate-adaptive immune cell crosstalk critically toward viral invasion improvement,highlighting a novel mechanism of virus-induced immunosuppression and providing new perspectives on the improvement of NDV-vectored vaccine.展开更多
文摘The contact problem for the elastic sphere indenting a layered half-space is considered. Analytical methods for solving this problem have been developed on the basis of the 3-D fundamental solution of a half space with a single coating layer under a normal concentrated force on the surface. The normal pressure distribution within the contact zone is assumed as Hertzian type. The solutions are constructed using superposition principle in the form of infinite series. Through comparing with the numerical results of FEM,it can be verified that the exact solutions have a rapid convergence rate and the stresses and displacements are mainly determined by the first term,which is corresponding to the solution of homogeneous half-space under Hertzian loading. The contact radius can be predicted applying the method.
文摘As a potential vectored vaccine,Newcastle disease virus(NDV)has been subject to various studies for vaccine development,while relatively little research has outlined the immunomodulatory effect of the virus in antigen presentation.To elucidate the key inhibitory factor in regulating the interaction of infected dendritic cells(DCs)and T cells,DCs were pretreated with the NDV vaccine strain LaSota as an inhibitor and stimulated with lipopolysaccharide(LPS)for further detection by enzyme-linked immunosorbent assay(ELISA),flow cytometry,immunoblotting,and quantitative real-time polymerase chain reaction(qRT-PCR).The results revealed that NDV infection resulted in the inhibition of interleukin(IL)-12p40 in DCs through a p38 mitogen-activated protein kinase(MAPK)-dependent manner,thus inhibiting the synthesis of IL-12p70,leading to the reduction in T cell proliferation and the secretion of interferon-(IFN-),tumor necrosis factor-α(TNF-α),and IL-6 induced by DCs.Consequently,downregulated cytokines accelerated the infection and viral transmission from DCs to T cells.Furthermore,several other strains of NDV also exhibited inhibitory activity.The current study reveals that NDV can modulate the intensity of the innate-adaptive immune cell crosstalk critically toward viral invasion improvement,highlighting a novel mechanism of virus-induced immunosuppression and providing new perspectives on the improvement of NDV-vectored vaccine.
