This paper focuses on the design of residential buildings oriented to the efficient use of solar energy,and selects the entries HUI HOUSE of Hefei University of Technology and Lille I University of France in the 3rd C...This paper focuses on the design of residential buildings oriented to the efficient use of solar energy,and selects the entries HUI HOUSE of Hefei University of Technology and Lille I University of France in the 3rd China International Solar Decathlon China Competition,based on the theory of the life cycle assessment(LCA)of buildings,and analyzes the carbon footprint from four aspects:building materials production and transportation stage,building construction stage,building operation stage,and building demolition stage.Through the calculation of the carbon footprint of buildings,the socio-economic benefits of HUI HOUSE in carbon reduction were analyzed;the result of the calculation was that HUI HOUSE achieved carbon neutrality in the ninth year,and continued carbon reduction after that,contributing a cumulative total of 947.54 tons of carbon negative in the life cycle of buildings.展开更多
目的:分析阿帕替尼治疗复发性和进展性韧带样纤维瘤(desmoid fibromatosis,DF)的疗效和安全性。方法:收集2017年10月至2020年9月就诊于四川大学华西医院的复发性或进展性韧带样纤维瘤患者37例,接受靶向药物阿帕替尼治疗,起始剂量250 mg...目的:分析阿帕替尼治疗复发性和进展性韧带样纤维瘤(desmoid fibromatosis,DF)的疗效和安全性。方法:收集2017年10月至2020年9月就诊于四川大学华西医院的复发性或进展性韧带样纤维瘤患者37例,接受靶向药物阿帕替尼治疗,起始剂量250 mg/d,根据药物不良反应调整剂量。结果:无完全缓解病例,17例(45.9%)为部分缓解,18例(48.6%)为疾病稳定,2例(5.4%)为疾病进展,客观有效率为45.9%,疾病控制率为94.6%。患者的关节活动度(rang of motion,ROM)、视觉模拟评分(visual analog scale,VAS)、上肢功能评定表(disabilities of the arm,shoulder and hand,DASH)评分、髋关节Harris评分及美国特种外科医院(HSS)评分均较治疗前有明显改善,且差异具有统计学意义(P<0.05)。常见的不良反应为手足综合征(37.8%)、胃肠反应(29.7%)和月经紊乱(21.6%)。结论:靶向药物阿帕替尼对复发性和进展性DF有一定的疗效,且不良反应可耐受,可作为复发性和进展性韧带样纤维瘤治疗的新途径。展开更多
Gut microbiota and circulating metabolite dysbiosis predate important pathological changes in glucose metabolic disorders;however,comprehensive studies on impaired glucose tolerance(IGT),a diabetes mellitus(DM)precurs...Gut microbiota and circulating metabolite dysbiosis predate important pathological changes in glucose metabolic disorders;however,comprehensive studies on impaired glucose tolerance(IGT),a diabetes mellitus(DM)precursor,are lacking.Here,we perform metagenomic sequencing and metabolomics on 47 pairs of individuals with IGT and newly diagnosed DM and 46 controls with normal glucose tolerance(NGT);patients with IGT are followed up after 4 years for progression to DM.Analysis of baseline data reveals significant differences in gut microbiota and serum metabolites among the IGT,DM,and NGT groups.In addition,13 types of gut microbiota and 17 types of circulating metabolites showed significant differences at baseline before IGT progressed to DM,including higher levels of Eggerthella unclassified,Coprobacillus unclassified,Clostridium ramosum,L-valine,L-norleucine,and L-isoleucine,and lower levels of Eubacterium eligens,Bacteroides faecis,Lachnospiraceae bacterium 3_1_46FAA,Alistipes senegalensis,Megaspaera elsdenii,Clostridium perfringens,α-linolenic acid,10E,12Z-octadecadienoic acid,and dodecanoic acid.A random forest model based on differential intestinal microbiota and circulating metabolites can predict the progression from IGT to DM(AUC=0.87).These results suggest that microbiome and metabolome dysbiosis occur in individuals with IGT and have important predictive values and potential for intervention in preventing IGT from progressing to DM.展开更多
The custom-tailored medicine requires a developmental strategy that integrates excellent osteogene-sis with mechanical stability to enhance the reconstruction of the critical-size bone defect(CSBD)and the healing proc...The custom-tailored medicine requires a developmental strategy that integrates excellent osteogene-sis with mechanical stability to enhance the reconstruction of the critical-size bone defect(CSBD)and the healing process in weight-bearing bone.We prepared three-dimensional(3D)printed biphasic cal-cium phosphate(BCP)scaffolds composited with nano-graphene oxide(GO).The biological effects of the GO/BCP composite scaffolds could induce the differentiation of rat bone marrow stem cells(BM-SCs)and the migration of human umbilical vein endothelial cells(HUVECs)for bone repair.The proper ratio of GO in the composite scaffold regulated the composites’surface roughness and hydrophilicity to a suitable range for the adhesion and proliferation of BMSCs and HUVECs.Besides,the GO/BCP composite scaffold increased osteogenesis and angiogenesis by activating BMP-2,RUNX-2,Smad1/4,and VEGF.The customized intramedullary nail combined with GO/BCP scaffold was applied to repair CSBD(2.0 cm in length)in a beagle femur model.This fixation strategy was confirmed by finite element analysis.In vivo,the results indicated that the custom-made internal fixation provided sufficient stability in the early stage,ensuring bone healing in a considerable mechanical environment.At 9 months postoperatively,longitudi-nal bony union and blood vessels in osteon were observed in the CSBD area with partial degradation in the 0.3%GO/BCP group.In the three-point bending test,the ultimate load of 0.3%GO/BCP group reached over 50%of the normal femur at 9 months after repair.These results showed a promising application of osteogenic GO/BCP scaffold and custom-made intramedullary nails in repairing CSBD of the beagle femur.This effective strategy could provide an option to treat the clinical CSBD in weight-bearing bones.展开更多
Wnt/β-catenin signaling has been broadly implicated in human cancers and experimental cancer models of animals.Aberrant activation of Wnt/β-catenin signaling is tightly linked with the increment of prevalence,advanc...Wnt/β-catenin signaling has been broadly implicated in human cancers and experimental cancer models of animals.Aberrant activation of Wnt/β-catenin signaling is tightly linked with the increment of prevalence,advancement of malignant progression,development of poor prognostics,and even ascendence of the cancer-associated mortality.Early experimental investigations have proposed the theoretical potential that efficient repression of this signaling might provide promising therapeutic choices in managing various types of cancers.Up to date,many therapies targeting Wnt/β-catenin signaling in cancers have been developed,which is assumed to endow clinicians with new opportunities of developing more satisfactory and precise remedies for cancer patients with aberrant Wnt/β-catenin signaling.展开更多
Digital light processing(DLP)-based 3D printing technique holds promise in fabricating scaffolds with high precision.Here raw calcium phosphate(CaP)powders were modified by 5.5%monoalcohol ethoxylate phosphate(MAEP)to...Digital light processing(DLP)-based 3D printing technique holds promise in fabricating scaffolds with high precision.Here raw calcium phosphate(CaP)powders were modified by 5.5%monoalcohol ethoxylate phosphate(MAEP)to ensure high solid loading and low viscosity.The rheological tests found that photocurable slurries composed of 50wt%modified CaP powders and 2wt%toners were suitable for DLP printing.Based on geometric models designed by computer-aided design(CAD)system,three printed CaP ceramics with distinct macroporous structures were prepared,including simple cube,octet-truss and inverse face-centered cube(fcc),which presented the similar phase composition and microstructure,but the different macropore geometries.Inverse fcc group showed the highest porosity and compressive strength.The in vitro and in vivo biological evaluations were performed to compare the bioactivity of three printed CaP ceramics,and the traditional foamed ceramic was used as control.It suggested that all CaP ceramics exhibited good biocompatibility,as evidence by an even bone-like apatite layer formation on the surface,and the good cell proliferation and spreading.A mouse intramuscular implantation model found that all of CaP ceramics could induce ectopic bone formation,and foam group had the strongest osteoinduction,followed by inverse fcc,while cube and octet-truss had the weakest one.It indicated that macropore geometry was of great importance to affect the osteoinductivity of scaffolds,and spherical,concave macropores facilitated osteogenesis.These findings provide a strategy to design and fabricate high-performance orthopedic grafts with proper pore geometry and desired biological performance via DLP-based 3D printing technique.展开更多
This study aimed at evaluating the possibility and effectiveness of osteoinductive bioceramics to fill the tumor cavity following the curettage of sacral giant cell tumor(GCT).Six patients(four females and two males,2...This study aimed at evaluating the possibility and effectiveness of osteoinductive bioceramics to fill the tumor cavity following the curettage of sacral giant cell tumor(GCT).Six patients(four females and two males,25–45 years old)underwent nervesparing surgery,in which the tumor was treated by denosumab,preoperative arterial embolization and extensive curettage.The remaining cavity was filled with commercial osteoinductive calcium phosphate(CaP)bioceramics,whose excellent osteoinductivity was confirmed by intramuscular implantation in beagle canine.All patients were followed by computed tomography(CT)scans postoperatively.According to the modified Neer criterion,five cases obtained Type I healing status,and one case had Type II.At the latest follow-up,no graft-related complications and local recurrence were found.The CT scan indicated a median time of healing initiation of 3months postoperatively,and the median time for relatively complete healing was 12months.The excellent bone regenerative ability of the ceramics was also confirmed by increased CT attenuation value,blurred boundary and cortical rim rebuilding.In conclusion,osteoinductive CaP bioceramics could be an ideal biomaterial to treat the large remaining cavity following extensive curettage of sacral GCT.However,further investigation with more cases and longer follow-up was required to confirm the final clinical effect.展开更多
文摘This paper focuses on the design of residential buildings oriented to the efficient use of solar energy,and selects the entries HUI HOUSE of Hefei University of Technology and Lille I University of France in the 3rd China International Solar Decathlon China Competition,based on the theory of the life cycle assessment(LCA)of buildings,and analyzes the carbon footprint from four aspects:building materials production and transportation stage,building construction stage,building operation stage,and building demolition stage.Through the calculation of the carbon footprint of buildings,the socio-economic benefits of HUI HOUSE in carbon reduction were analyzed;the result of the calculation was that HUI HOUSE achieved carbon neutrality in the ninth year,and continued carbon reduction after that,contributing a cumulative total of 947.54 tons of carbon negative in the life cycle of buildings.
文摘目的:分析阿帕替尼治疗复发性和进展性韧带样纤维瘤(desmoid fibromatosis,DF)的疗效和安全性。方法:收集2017年10月至2020年9月就诊于四川大学华西医院的复发性或进展性韧带样纤维瘤患者37例,接受靶向药物阿帕替尼治疗,起始剂量250 mg/d,根据药物不良反应调整剂量。结果:无完全缓解病例,17例(45.9%)为部分缓解,18例(48.6%)为疾病稳定,2例(5.4%)为疾病进展,客观有效率为45.9%,疾病控制率为94.6%。患者的关节活动度(rang of motion,ROM)、视觉模拟评分(visual analog scale,VAS)、上肢功能评定表(disabilities of the arm,shoulder and hand,DASH)评分、髋关节Harris评分及美国特种外科医院(HSS)评分均较治疗前有明显改善,且差异具有统计学意义(P<0.05)。常见的不良反应为手足综合征(37.8%)、胃肠反应(29.7%)和月经紊乱(21.6%)。结论:靶向药物阿帕替尼对复发性和进展性DF有一定的疗效,且不良反应可耐受,可作为复发性和进展性韧带样纤维瘤治疗的新途径。
基金supported by the Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(ZYYCXTD-D-202001)the National Natural Science Foundation of China(82104835).
文摘Gut microbiota and circulating metabolite dysbiosis predate important pathological changes in glucose metabolic disorders;however,comprehensive studies on impaired glucose tolerance(IGT),a diabetes mellitus(DM)precursor,are lacking.Here,we perform metagenomic sequencing and metabolomics on 47 pairs of individuals with IGT and newly diagnosed DM and 46 controls with normal glucose tolerance(NGT);patients with IGT are followed up after 4 years for progression to DM.Analysis of baseline data reveals significant differences in gut microbiota and serum metabolites among the IGT,DM,and NGT groups.In addition,13 types of gut microbiota and 17 types of circulating metabolites showed significant differences at baseline before IGT progressed to DM,including higher levels of Eggerthella unclassified,Coprobacillus unclassified,Clostridium ramosum,L-valine,L-norleucine,and L-isoleucine,and lower levels of Eubacterium eligens,Bacteroides faecis,Lachnospiraceae bacterium 3_1_46FAA,Alistipes senegalensis,Megaspaera elsdenii,Clostridium perfringens,α-linolenic acid,10E,12Z-octadecadienoic acid,and dodecanoic acid.A random forest model based on differential intestinal microbiota and circulating metabolites can predict the progression from IGT to DM(AUC=0.87).These results suggest that microbiome and metabolome dysbiosis occur in individuals with IGT and have important predictive values and potential for intervention in preventing IGT from progressing to DM.
基金This work was financially supported,in part,by the Sci-ence and Technology Research Program of Sichuan Province(No.2020YFS0036,Dr.Tu)the 1·3·5 project for disciplines of excel-lence,West China Hospital,Sichuan University(No.ZYJC18036,Dr.Tu).
文摘The custom-tailored medicine requires a developmental strategy that integrates excellent osteogene-sis with mechanical stability to enhance the reconstruction of the critical-size bone defect(CSBD)and the healing process in weight-bearing bone.We prepared three-dimensional(3D)printed biphasic cal-cium phosphate(BCP)scaffolds composited with nano-graphene oxide(GO).The biological effects of the GO/BCP composite scaffolds could induce the differentiation of rat bone marrow stem cells(BM-SCs)and the migration of human umbilical vein endothelial cells(HUVECs)for bone repair.The proper ratio of GO in the composite scaffold regulated the composites’surface roughness and hydrophilicity to a suitable range for the adhesion and proliferation of BMSCs and HUVECs.Besides,the GO/BCP composite scaffold increased osteogenesis and angiogenesis by activating BMP-2,RUNX-2,Smad1/4,and VEGF.The customized intramedullary nail combined with GO/BCP scaffold was applied to repair CSBD(2.0 cm in length)in a beagle femur model.This fixation strategy was confirmed by finite element analysis.In vivo,the results indicated that the custom-made internal fixation provided sufficient stability in the early stage,ensuring bone healing in a considerable mechanical environment.At 9 months postoperatively,longitudi-nal bony union and blood vessels in osteon were observed in the CSBD area with partial degradation in the 0.3%GO/BCP group.In the three-point bending test,the ultimate load of 0.3%GO/BCP group reached over 50%of the normal femur at 9 months after repair.These results showed a promising application of osteogenic GO/BCP scaffold and custom-made intramedullary nails in repairing CSBD of the beagle femur.This effective strategy could provide an option to treat the clinical CSBD in weight-bearing bones.
基金The work is supported by the National Natural Science Foundation of China 81825005State Key Laboratory of Oral Diseases SKLOD202007(L.Y.).
文摘Wnt/β-catenin signaling has been broadly implicated in human cancers and experimental cancer models of animals.Aberrant activation of Wnt/β-catenin signaling is tightly linked with the increment of prevalence,advancement of malignant progression,development of poor prognostics,and even ascendence of the cancer-associated mortality.Early experimental investigations have proposed the theoretical potential that efficient repression of this signaling might provide promising therapeutic choices in managing various types of cancers.Up to date,many therapies targeting Wnt/β-catenin signaling in cancers have been developed,which is assumed to endow clinicians with new opportunities of developing more satisfactory and precise remedies for cancer patients with aberrant Wnt/β-catenin signaling.
基金sponsored by the National Key Research and Development Program of China(2017YFB0702600)National Natural Science Foundation of China(31971283,31670985)Sichuan Science and Technology Programs(2019JDTD0008,2021YFS0032).
文摘Digital light processing(DLP)-based 3D printing technique holds promise in fabricating scaffolds with high precision.Here raw calcium phosphate(CaP)powders were modified by 5.5%monoalcohol ethoxylate phosphate(MAEP)to ensure high solid loading and low viscosity.The rheological tests found that photocurable slurries composed of 50wt%modified CaP powders and 2wt%toners were suitable for DLP printing.Based on geometric models designed by computer-aided design(CAD)system,three printed CaP ceramics with distinct macroporous structures were prepared,including simple cube,octet-truss and inverse face-centered cube(fcc),which presented the similar phase composition and microstructure,but the different macropore geometries.Inverse fcc group showed the highest porosity and compressive strength.The in vitro and in vivo biological evaluations were performed to compare the bioactivity of three printed CaP ceramics,and the traditional foamed ceramic was used as control.It suggested that all CaP ceramics exhibited good biocompatibility,as evidence by an even bone-like apatite layer formation on the surface,and the good cell proliferation and spreading.A mouse intramuscular implantation model found that all of CaP ceramics could induce ectopic bone formation,and foam group had the strongest osteoinduction,followed by inverse fcc,while cube and octet-truss had the weakest one.It indicated that macropore geometry was of great importance to affect the osteoinductivity of scaffolds,and spherical,concave macropores facilitated osteogenesis.These findings provide a strategy to design and fabricate high-performance orthopedic grafts with proper pore geometry and desired biological performance via DLP-based 3D printing technique.
文摘This study aimed at evaluating the possibility and effectiveness of osteoinductive bioceramics to fill the tumor cavity following the curettage of sacral giant cell tumor(GCT).Six patients(four females and two males,25–45 years old)underwent nervesparing surgery,in which the tumor was treated by denosumab,preoperative arterial embolization and extensive curettage.The remaining cavity was filled with commercial osteoinductive calcium phosphate(CaP)bioceramics,whose excellent osteoinductivity was confirmed by intramuscular implantation in beagle canine.All patients were followed by computed tomography(CT)scans postoperatively.According to the modified Neer criterion,five cases obtained Type I healing status,and one case had Type II.At the latest follow-up,no graft-related complications and local recurrence were found.The CT scan indicated a median time of healing initiation of 3months postoperatively,and the median time for relatively complete healing was 12months.The excellent bone regenerative ability of the ceramics was also confirmed by increased CT attenuation value,blurred boundary and cortical rim rebuilding.In conclusion,osteoinductive CaP bioceramics could be an ideal biomaterial to treat the large remaining cavity following extensive curettage of sacral GCT.However,further investigation with more cases and longer follow-up was required to confirm the final clinical effect.
