The pathogenic effects of type 2 diabetes on bone tissue are gaining attention,but the cellular and molecular mechanisms underlying osteoimmunology are still unclear in diabetes-related bone diseases.We delineated the...The pathogenic effects of type 2 diabetes on bone tissue are gaining attention,but the cellular and molecular mechanisms underlying osteoimmunology are still unclear in diabetes-related bone diseases.We delineated the single-cell transcriptome of bone marrow cells from both wide type and type 2 diabetes mice,which provided the first detailed global profile of bone marrow cells and revealed a distinct bone immune microenvironment at the genetic level under type 2 diabetic condition.It was observed that osteoclast activity was inhibited due to a dysre-gulated cytokine network,which ultimately led to decreased osteoclast formation and differen-tiation.In type 2 diabetes mice,a specific Cd36+cluster(cluster 18,monocytes/macrophages 2)was identified as the precursor of osteoclasts with diminished differentiation potential.AP-1 was demonstrated to be the key transcription factor in the underlying mechanism.展开更多
基金supported by the National Natural Science Foundation of China (No.81972045)the Basic Applied Basic Research Foundation of Guangdong Province,China (No.2022A1515012373)the research fund from SUSTech Hospital.
文摘The pathogenic effects of type 2 diabetes on bone tissue are gaining attention,but the cellular and molecular mechanisms underlying osteoimmunology are still unclear in diabetes-related bone diseases.We delineated the single-cell transcriptome of bone marrow cells from both wide type and type 2 diabetes mice,which provided the first detailed global profile of bone marrow cells and revealed a distinct bone immune microenvironment at the genetic level under type 2 diabetic condition.It was observed that osteoclast activity was inhibited due to a dysre-gulated cytokine network,which ultimately led to decreased osteoclast formation and differen-tiation.In type 2 diabetes mice,a specific Cd36+cluster(cluster 18,monocytes/macrophages 2)was identified as the precursor of osteoclasts with diminished differentiation potential.AP-1 was demonstrated to be the key transcription factor in the underlying mechanism.
