茶多酚的经皮渗透特征及其对UVA致HSF氧化损伤的保护作用

研究了茶多酚的经皮渗透特征,探究茶多酚对长波紫外线(UVA)诱导的人皮肤成纤维细胞氧化损伤的保护作用机理。采用Franz扩散池,以大鼠皮肤为屏障,建立了完善的检测方法,用高效液相色谱检测茶多酚中儿茶素、表儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯的经皮渗透指标;通过建立UVA损伤HSF细胞模型,探究了茶多酚对细胞活性氧含量、丙二醛水平、超氧化物歧化酶和谷胱甘肽过氧化物酶活力的影响。结果表明该检测方法具有较好的专属性、精密度和加样回收率,且茶多酚中的儿茶素、表儿茶素、表儿茶素没食子酸酯、表没食子儿茶素没食子酸酯4种物质均可较好地作用于皮肤,其中表儿茶素在透皮释放方面具有明显优势。茶多酚通过降低UVA辐照后HSF细胞中的ROS含量和MDA水平,可提高细胞内SOD和GSH-Px活力,对UVA诱导的HSF细胞氧化损伤具有良好的保护作用。

Simplified methods to isolate,culture and purify olfactory ensheathing cells

Conventional methods for harvesting, culturing and purifying olfactory ensheathing cells are complicated, time-consuming, and poorly reproducible. Olfactory bulbs were detached from adult Sprague Dawley rats and olfactory ensheathing cells were isolated using shearing, dispersion processes. After the primary cultures reached confluence, the cells were purified using a three-step process. The olfactory ensheathing cells attached and grew rapidly. The purity of the olfactory ensheathing cells increased following the three purification steps, eventually exceeding 95%. These cells could be maintained for an extended period time in culture. This simple, inexpensive, reproducible method of harvesting, culturing and purifying olfactory ensheathing cells shortens the culture cycle and provides sufficient olfactory ensheathing cells of controllable purity.

Influence of acupuncture with exercise training on learning and memory functions, as well as microtubule-associated protein-2 and synaptophysin expression in the hippocampal CA3 region, in a rat model of cerebral infarction

The present study was designed to determine microtubule-associated protein-2 and synaptophysin expression in the hippocampal CA3 region in a rat model of middle cerebral artery occlusion. The rats were treated with acupuncture at Baihui （GV 20）, Qubin （GB 7）, and Qianding （GV 21） points, in addition to exercise training. Results were compared with rats undergoing exercise training only. The Y-maze method and immunohistochemistry revealed decreased error frequency of passing through Y-maze, as well as significantly increased microtubule-associated protein-2 and synaptophysin expression, in the acupuncture with exercise training group compared with the model and exercise training groups after 5 weeks. Microtubule-associated protein-2 and synaptophysin expressions negatively correlated with error frequency of passing through the Y-maze. These results suggested that acupuncture combined with exercise training improved learning and memory functions in a rat model of cerebral infarction. The mechanisms of action were hypothesized to be associated with dendritic or synaptic plasticity in the ipsilateral hippocampal CA3 region.

Impact analysis of expressway construction on ecological carrying capacity in the Three-River Headwater Region

The construction of expressways has a variety of impacts on the ecologically fragile and sensitive Three-River Headwater Region and causes changes in the structure and carrying capacity of the ecosystem. To assess the impact of expressway construction on the ecological carrying capacity of the regional natural system, net primary productivity(NPP)was used as the quantitative index to indicate the efficiency of the regional ecological environment to fix and transform photosynthesis products. In this study, trend analysis and buffer zone analysis were used to quantitatively analyze the spatial and temporal characteristics of NPP in the study area from 2006 to 2015 based on the MOD17A3/NPP dataset. In addition, the spatial-temporal effects of expressway and interchange construction on NPP in buffer zones were studied using GIS spatial analysis. The summarized results are as follows.(1) The multi-year average NPP in the study area is 137.9 gC/m^2,indicating a slight downward trend;spatially, the overall NPP shows a downward trend from east to west.(2) The impact of expressway construction on NPP in buffer zones will last for more than two years, generally presenting greater NPP further from the expressway.(3) After completion of the interchange, the NPP decreases significantly within its radiation radius;at a distance of 6 km, the NPP gradually stabilizes.(4) The influence law of expressway construction and interchange construction on NPP is approximately the same in the respective buffer zones.

Concurrent validity of evaluating knee kinematics using Kinect system during rehabilitation exercise

Squats,ascending and descending stairs,and lunges are three functional movements popularly used in clinical rehabilitation.This study aimed to analyze the validity of the Kinect v2 for assessing the kinematics of three functional knee movements.An intelligent program was developed for real-time kinematic evaluation of the knee joint using the Kinect v2 system.Based on image recognition technology,images from the Kinect v2 depth camera were used to develop a kinematic evaluation program for knee joint movements.The kinematics of 30 healthy volunteers during stair ascent and descent,squatting,and lunging movements were collected synchronously with both VICON and Kinect systems.The peak knee angles in the sagittal and coronal planes were extracted and analyzed.A paired samples t-test and the relative coefficient within the groups were used for statistical analysis of the validity of the Kinect compared to the VICON.There was no significant difference(p>0.05)in bilateral knee flexion angle when lunging.No significant difference(p>0.05)was found in left knee flexion during squatting exercises.Although the Kinect generally underestimated knee flexion and valgus,it displayed excellent consistency and good to excellent agreement with the VICON motion analysis system.The results demonstrated that the Kinect based measurement tool for analyzing knee kinematics has the potential to be an effective clinical training device for maintaining knee alignment during exercise.