An edge Lyman-alpha-based beam emission spectroscopy(LyBES)diagnostic,using a heating NBI(neutral beam injection)system,is currently under development on the HL-2A tokamak.The 20-channel edge LyBES,which is intended t...An edge Lyman-alpha-based beam emission spectroscopy(LyBES)diagnostic,using a heating NBI(neutral beam injection)system,is currently under development on the HL-2A tokamak.The 20-channel edge LyBES,which is intended to measure the density fluctuation in plasma edge(from R=1960 mm to R=2026 mm)with an improved spatial resolution of 3.3 mm,is a complement to the existing conventional beam emission spectroscopy(BES)diagnostic.In this article,we introduce the progress of LyBES diagnostic,including the collection optics,the monochromator,and the detector system.The reflectance of the collection mirrors is measured to be~82%at 122 nm,and the aberration geometrical radius of the collection optics is tested to be~150μm in the aimed area.The linear dispersion of the LyBES monochromator is designed to be~0.09 nm mm^(-1).The bandwidth of the detector system with the 5×10^(7)V A^(-1)preamplifier gain is measured to be~280 kHz,and the peak-to-peak noise of the detector system is tested to be~16 mV.The finalized design,components development and testing of the LyBES diagnostic have been completed at present,and an overall performance of the LyBES diagnostic is to be confirmed in the next HL-2A campaign.展开更多
The optical design of near-infrared phase contrast imaging(NI-PCI)diagnosis on HL-2A is introduced in this paper.This scheme benefits from the great progress of near-infrared laser technology and is a broadening of tr...The optical design of near-infrared phase contrast imaging(NI-PCI)diagnosis on HL-2A is introduced in this paper.This scheme benefits from the great progress of near-infrared laser technology and is a broadening of traditional phase contrast technology.This diagnostic can work as a keen tool to measure plasma wavenumber spectra by inferring string-integrated plasma density fluctuations.Design of both the front optical path which is the path before the laser transmitting into the tokamak plasma and the rear optics which is the path after the laser passing through the plasma is detailed.The 1550 nm laser is chosen as the probe beam and highprecision optical components are designed to fit the laser beam,in which a phase plate with a 194-nm-deep silver groove is the key.Compared with the conventional 10.6μm laser-based PCI system on HL-2A,NI-PCI significantly overcomes the unwanted phase scintillation effect and promotes the measurement capability of high-wavenumber turbulence with an increased maximal measurable wavenumber from 15 cm^(-1)to 32.6 cm^(-1).展开更多
Melon(Cucumis melo L.)has a long history of cultivation worldwide.During cultivation,domestication,and selection breeding,the sugar content of mature melon fruits has been significantly increased.Compared with unsweet...Melon(Cucumis melo L.)has a long history of cultivation worldwide.During cultivation,domestication,and selection breeding,the sugar content of mature melon fruits has been significantly increased.Compared with unsweet melon and wild melon,rapid sucrose accumulation can occur in the middle and late stages of sweet melon fruit development.The phloem unloading pathway during the evolution and development of melon fruit has not been identified and analyzed.In this study,the phloem unloading pathway and the function of related sugar transporters in cultivated and wild melon fruits were analyzed by CFDA[5(6)-carbofluorescein diacetate]and esculin tracing,cytological pathway observation,qRT–PCR,and gene function analysis,etc.Results show that the phloem unloading pathway of wild melon fruit is largely symplastic,whereas the phloem unloading pathway of cultivated melon fruit shifts from symplastic to apoplasmic during development.According to a fruit grafting experiment,the fruit sink accumulates sugars independently.Correlation analysis showed that the expression amounts of several sucrose transporter genes were positively correlated with the sucrose content of melon fruit.Furthermore,CmSWEET10 was proved to be a sucrose transporter located on the plasma membrane of the phloem and highly expressed in the premature stage of sweet melon fruits,which means it may be involved in phloem apoplast unloading and sucrose accumulation in sweet melon fruits.Finally,we summarize a functional model of related enzymes and sugar transporters involved in the apoplast unloading of sweet melon fruits during enlargement and sucrose accumulation.展开更多
Tin halide perovskites recently have attracted extensive research attention due to their similar electronic and band structures but non-toxicity compared with their lead analogues. In this work, we prepare high-qualit...Tin halide perovskites recently have attracted extensive research attention due to their similar electronic and band structures but non-toxicity compared with their lead analogues. In this work, we prepare high-quality CsSnX_(3)(X=Br,I) microplates with lateral sizes of around 1–4 μm by chemical vapor deposition and investigate their low-temperature photoluminescence(PL) properties. A remarkable splitting of PL peaks of the CsSnBr_(3)microplate is observed at low temperatures. Besides the possible structural phase transition at below 70 K, the multi-peak fittings using Gauss functions and the power-dependent saturation phenomenon suggest that the PL could also be influenced by the conversion from the emission of bound excitons into free excitons. With the increase of temperature, the peak position shows a blueshift tendency for CsSnI_(3), which is governed by thermal expansion. However, the peak position of the CsSnBr3microplate exhibits a transition from redshift to blueshift at ~160 K. The full width at half maximum of CsSnX_(3)broadens with increasing temperature, and the fitting results imply that longitudinal optical phonons dominate the electron–phonon coupling and the coupling strength is much more robust in CsSnBr3than in CsSnI_(3). The PL intensity of CsSnX_(3)microplates is suppressed due to the enhanced non-radiative relaxation and exciton dissociation competing with radiative recombination. According to the Arrhenius law, the exciton binding energy of CsSnBr_(3)is ~38.4 meV, slightly smaller than that of CsSnI_(3).展开更多
In this article,the design of a Lyman-alpha-based beam emission spectroscopy(LAB)diagnostic on the HL-2A tokamak has been proposed for the first time.The purpose of this novel diagnostic is to measure density fluctuat...In this article,the design of a Lyman-alpha-based beam emission spectroscopy(LAB)diagnostic on the HL-2A tokamak has been proposed for the first time.The purpose of this novel diagnostic is to measure density fluctuations of tokamak plasma.The light-collection system of LAB,which consists of the first mirror and two groups of coaxial double-mirror telescopes,can realize a twosegmented viewing field ofρ=0–0.2 andρ=0.75–1,which is optimized to measure plasma density fluctuation,not only in the edge transport barrier region but also in the internal transport barrier region,to investigate the underlying physics of turbulence in tokamaks.Spectrometers are developed to separate out the Doppler-shifted target line(122.03 and 122.17 nm)from the background Lyman-alpha line(121.53 nm).Here,30 Core-LAB channels and 30 Edge-LAB channels are under development on the HL-2A tokamak.It has high radial spatial resolutions of about 2.7 mm and 3.3 mm for the core and edge channels,respectively.Taking the high light intensity of this Lyman-alpha line into account,temporal resolution of 200 k Hz can be ensured by broad bandwidth amplifiers.This high spatio-temporal resolution makes LAB a potential keen tool to experimentally investigate tokamak plasma physics.展开更多
A vertical edge Thomson scattering(ETS) diagnostic system on HL-2M tokamak has been designed.The ETS system collects the scattered light from Nd:YAG laser(1064 nm,2 J,30 Hz,15 ns).The laser beam propagates vertically ...A vertical edge Thomson scattering(ETS) diagnostic system on HL-2M tokamak has been designed.The ETS system collects the scattered light from Nd:YAG laser(1064 nm,2 J,30 Hz,15 ns).The laser beam propagates vertically through the plasma region and the polarization is parallel to the toroidal magnetic field.A special designed Galileo-type telescope with long Rayleigh length is applied to focus the laser size and ensure collimation.A group of doubleGaussian collection lenses image the 600 mm vertical scattered region onto rectangular fiber arrays with a spatial resolution of 10 mm.The 2.20 mm × 2.86 mm fiber optic bundle consists of 130 low hydroxyl(OH) 200/220 μm(core/cladding) diameter fibers with numerical aperture NA=0.22,carrying the light to remotely located multi-channel polychromators.Effect of oblique incidence on narrow band filter has been analyzed.The designed electron temperatures range from 5 to 1000 eV and electron densities from 5 × 10^(18) to 1×10^(20) m^(-3).展开更多
Ion temperature, as one of the most critical plasma parameters, can be diagnosed by charge exchange recombination spectroscopy (CXRS). Iterative least-squares fitting is conventionally used to analyze CXRS spectra to ...Ion temperature, as one of the most critical plasma parameters, can be diagnosed by charge exchange recombination spectroscopy (CXRS). Iterative least-squares fitting is conventionally used to analyze CXRS spectra to identify the active charge exchange component, which is the result of local interaction between impurity ions with a neutral beam. Due to the limit of the time consumption of the conventional approach (~100 ms per frame), the Experimental Advanced Superconducting Tokamak CXRS data is now analyzed in-between shots. To explore the feasibility of real-time measurement, neural networks are introduced to perform fast estimation of ion temperature. Based on the same four-layer neural network architecture, two neural networks are trained for two central chords according to the ion temperature data acquired from the conventional method. Using the TensorFlow framework, the training procedures are performed by an error back-propagation algorithm with the regularization via the weight decay method. Good agreement in the deduced ion temperature is shown for the neural networks and the conventional approach, while the data processing time is reduced by 3 orders of magnitude (~0.1 ms per frame) by using the neural networks.展开更多
In this article,we present the optical design of a novel diagnostic on the HL-2A tokamak,i.e.the 20-channel edge Lyman-alpha beam emission spectroscopy,which is a promising solution for edge density turbulence researc...In this article,we present the optical design of a novel diagnostic on the HL-2A tokamak,i.e.the 20-channel edge Lyman-alpha beam emission spectroscopy,which is a promising solution for edge density turbulence research on tokamaks,as it offers the possibility of density fluctuation measurement with a 3.3 mm spatial resolution while maintains a high temporal resolution of 1μs.The optical path,including the reflective collection optics,the high-dispersion spectrometer,and the linear detector array,is carefully optimized to obtain a good image quality and a high throughput.The maximum root mean square radius of the collection optics is 64μm.The detected photon flux is estimated to be about 1011 photons/s/channel.展开更多
【目的】丝氨酸/苏氨酸蛋白激酶K(Serine/Threonine protein kinases K)是分枝杆菌类似真核样的蛋白激酶,预测在分枝杆菌的生长和新陈代谢等生理过程中起着重要的作用,解析PknK的生物功能及作用机制,将为结核病的防治提供一定的理论基...【目的】丝氨酸/苏氨酸蛋白激酶K(Serine/Threonine protein kinases K)是分枝杆菌类似真核样的蛋白激酶,预测在分枝杆菌的生长和新陈代谢等生理过程中起着重要的作用,解析PknK的生物功能及作用机制,将为结核病的防治提供一定的理论基础。【方法】通过基因敲除等遗传方法获得结核分枝杆菌疫苗株BCG的pknK敲除菌株△pknK、回补菌株pMV361-pknK/△pknK和过表达菌株pMV261-pknK/BCG;对获得的菌株进行生长曲线测定和抗药性分析;通过pulldown-MS方法及生物信息学方法鉴定了PknK相互作用蛋白。【结果】监测各种分枝杆菌△pknK、pMV361-pknK/△pknK和pMV261-pknK/BCG生长,确定PknK负调控BCG生长;抗药性分析显示PknK降低BCG的耐药性;pulldown-MS方法显示PknK与丝氨酸/苏氨酸蛋白激酶PknA和双组分系统中的反应调节因子MtrA、TrcR、MoxR等蛋白相互作用。【结论】研究发现PknK调控分枝杆菌的生长和耐药性,我们的研究为深入研究PknK在结核分枝杆菌中的功能奠定了基础。展开更多
Understanding and controlling defect in two-dimensional materials is important for both linear and nonlinear optoelectronic devices,especially in terms of tuning nonlinear optical absorption.Taking advantage of an ato...Understanding and controlling defect in two-dimensional materials is important for both linear and nonlinear optoelectronic devices,especially in terms of tuning nonlinear optical absorption.Taking advantage of an atomic defect formed easily by smaller size,molybdenum disulfide nanosheet is prepared successfully with a different size by gradient centrifugation.Interestingly,size-dependent sulfur vacancies are observed by high-resolution X-ray photoelectron spectroscopy,atomic force microscopy,and transmission electron microscopy.The defect effect on nonlinear absorption is investigated by Z-scan measurement at the wavelength of 800 nm.The results suggest the transition from saturable absorption to reverse saturable absorption can be observed in both dispersions and films.First principle calculations suggest that sulfur vacancies act as the trap state to capture the excited electrons.Moreover,an energy-level model with the trap state is put forward to explain the role of the sulfur vacancy defect in nonlinear optical absorption.The results suggest that saturable absorption and reverse saturable absorption originate from the competition between the excited,defect state and ground state absorption.Our finding provides a way to tune the nonlinear optical performance of optoelectronic devices by defect engineering.展开更多
Heterostructures based on new advanced materials offer a cornerstone for future optoelectronic devices with improved photoelectric performance.Band alignment is crucial for understanding the mechanism of charge carrie...Heterostructures based on new advanced materials offer a cornerstone for future optoelectronic devices with improved photoelectric performance.Band alignment is crucial for understanding the mechanism of charge carrier transportation and interface dynamics in heterostructures.Herein,we grew SnS_(2)/Bi_(2)X_(3)(X=Se,Te)van der Waals heterostructures by combining physical vapor deposition with chemical vapor deposition.The band alignment,measured by high-resolution X-ray photoelectron spectroscopy,suggested the successful design of type-Ⅰ SnS_(2)/Bi_(2)Te_(3) and type-Ⅱ SnS_(2)/Bi_(2)Te_(3) heterostructures.The SnS_(2)/Bi_(2)X_(3) heterostructure greatly improved the photoelectric response of a photoelectrochemical-type photodetector.The photocurrent densities in the type-Ⅰ SnS_(2)/Bi_(2)Te_(3) and type-Ⅱ SnS_(2)/Bi_(2)Te_(3) heterostructure-based devices were more than one order of magnitude higher than those of SnS_(2),Bi_(2)Te_(3),and Bi_(2)Te_(3).The improved photoelectric properties of the SnS_(2)/Bi_(2)X_(3) heterostructures can be explained as follows:(i)the photoexcited electrons and holes are effectively separated in the heterostructures;(ii)the charge-transfer efficiency and carrier density at the interface between the SnS_(2)/Bi_(2)X_(3) heterostructures and the electrolyte are greatly improved;(iii)the formed heterostructures expand the light absorption range.The photoelectric performance was further enhanced by efficient light trapping in the upright SnS_(2).The photoelectric response is higher in the type-Ⅰ SnS_(2)/Bi_(2)Te_(3) heterostructure than in the type-Ⅱ SnS_(2)/Bi_(2)Te_(3) heterostructure due to more efficient charge transportation at the type-Ⅰ SnS_(2)/Bi_(2)Te_(3) heterostructure/electrolyte interface.These results suggest that suitable type-Ⅰ and type-Ⅱ heterostructures can be developed for high-performance photodetectors and other optoelectronic devices.展开更多
基金supported by Sichuan Science and Technology Program(No.2022JDJQ0038)the National Key R&D Program of China(Nos.2022YFE03100002 and 2018YFE0303102)National Natural Science Foundation of China(Nos.12205087 and 12075241)。
文摘An edge Lyman-alpha-based beam emission spectroscopy(LyBES)diagnostic,using a heating NBI(neutral beam injection)system,is currently under development on the HL-2A tokamak.The 20-channel edge LyBES,which is intended to measure the density fluctuation in plasma edge(from R=1960 mm to R=2026 mm)with an improved spatial resolution of 3.3 mm,is a complement to the existing conventional beam emission spectroscopy(BES)diagnostic.In this article,we introduce the progress of LyBES diagnostic,including the collection optics,the monochromator,and the detector system.The reflectance of the collection mirrors is measured to be~82%at 122 nm,and the aberration geometrical radius of the collection optics is tested to be~150μm in the aimed area.The linear dispersion of the LyBES monochromator is designed to be~0.09 nm mm^(-1).The bandwidth of the detector system with the 5×10^(7)V A^(-1)preamplifier gain is measured to be~280 kHz,and the peak-to-peak noise of the detector system is tested to be~16 mV.The finalized design,components development and testing of the LyBES diagnostic have been completed at present,and an overall performance of the LyBES diagnostic is to be confirmed in the next HL-2A campaign.
基金supported by the National Key Research and Development Program of China(Nos.2019YFE03090100 and 2022YFE03100002)National Natural Science Foundation of China(No.12075241)。
文摘The optical design of near-infrared phase contrast imaging(NI-PCI)diagnosis on HL-2A is introduced in this paper.This scheme benefits from the great progress of near-infrared laser technology and is a broadening of traditional phase contrast technology.This diagnostic can work as a keen tool to measure plasma wavenumber spectra by inferring string-integrated plasma density fluctuations.Design of both the front optical path which is the path before the laser transmitting into the tokamak plasma and the rear optics which is the path after the laser passing through the plasma is detailed.The 1550 nm laser is chosen as the probe beam and highprecision optical components are designed to fit the laser beam,in which a phase plate with a 194-nm-deep silver groove is the key.Compared with the conventional 10.6μm laser-based PCI system on HL-2A,NI-PCI significantly overcomes the unwanted phase scintillation effect and promotes the measurement capability of high-wavenumber turbulence with an increased maximal measurable wavenumber from 15 cm^(-1)to 32.6 cm^(-1).
基金This work was supported by the National Natural Science Foundation of China(31972435)the National Key Research and Development Program of China(2019YFD1000300)+1 种基金the Fundamental Research Funds for the Central Universities(2662018QD062)the China Agriculture Research System of MOF and MORA(CARS-25).
文摘Melon(Cucumis melo L.)has a long history of cultivation worldwide.During cultivation,domestication,and selection breeding,the sugar content of mature melon fruits has been significantly increased.Compared with unsweet melon and wild melon,rapid sucrose accumulation can occur in the middle and late stages of sweet melon fruit development.The phloem unloading pathway during the evolution and development of melon fruit has not been identified and analyzed.In this study,the phloem unloading pathway and the function of related sugar transporters in cultivated and wild melon fruits were analyzed by CFDA[5(6)-carbofluorescein diacetate]and esculin tracing,cytological pathway observation,qRT–PCR,and gene function analysis,etc.Results show that the phloem unloading pathway of wild melon fruit is largely symplastic,whereas the phloem unloading pathway of cultivated melon fruit shifts from symplastic to apoplasmic during development.According to a fruit grafting experiment,the fruit sink accumulates sugars independently.Correlation analysis showed that the expression amounts of several sucrose transporter genes were positively correlated with the sucrose content of melon fruit.Furthermore,CmSWEET10 was proved to be a sucrose transporter located on the plasma membrane of the phloem and highly expressed in the premature stage of sweet melon fruits,which means it may be involved in phloem apoplast unloading and sucrose accumulation in sweet melon fruits.Finally,we summarize a functional model of related enzymes and sugar transporters involved in the apoplast unloading of sweet melon fruits during enlargement and sucrose accumulation.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 11974279, 12074311, 12004310, and 12261141662)。
文摘Tin halide perovskites recently have attracted extensive research attention due to their similar electronic and band structures but non-toxicity compared with their lead analogues. In this work, we prepare high-quality CsSnX_(3)(X=Br,I) microplates with lateral sizes of around 1–4 μm by chemical vapor deposition and investigate their low-temperature photoluminescence(PL) properties. A remarkable splitting of PL peaks of the CsSnBr_(3)microplate is observed at low temperatures. Besides the possible structural phase transition at below 70 K, the multi-peak fittings using Gauss functions and the power-dependent saturation phenomenon suggest that the PL could also be influenced by the conversion from the emission of bound excitons into free excitons. With the increase of temperature, the peak position shows a blueshift tendency for CsSnI_(3), which is governed by thermal expansion. However, the peak position of the CsSnBr3microplate exhibits a transition from redshift to blueshift at ~160 K. The full width at half maximum of CsSnX_(3)broadens with increasing temperature, and the fitting results imply that longitudinal optical phonons dominate the electron–phonon coupling and the coupling strength is much more robust in CsSnBr3than in CsSnI_(3). The PL intensity of CsSnX_(3)microplates is suppressed due to the enhanced non-radiative relaxation and exciton dissociation competing with radiative recombination. According to the Arrhenius law, the exciton binding energy of CsSnBr_(3)is ~38.4 meV, slightly smaller than that of CsSnI_(3).
基金supported by the National Key R&D Program of China(Nos.2022YFE03100002,2017YFE0301201 and 2018YFE0303102)Sichuan Science and Technology Program(Nos.2022JDJQ0038 and 2020JDTD0030)National Natural Science Foundation of China(No.12205087)。
文摘In this article,the design of a Lyman-alpha-based beam emission spectroscopy(LAB)diagnostic on the HL-2A tokamak has been proposed for the first time.The purpose of this novel diagnostic is to measure density fluctuations of tokamak plasma.The light-collection system of LAB,which consists of the first mirror and two groups of coaxial double-mirror telescopes,can realize a twosegmented viewing field ofρ=0–0.2 andρ=0.75–1,which is optimized to measure plasma density fluctuation,not only in the edge transport barrier region but also in the internal transport barrier region,to investigate the underlying physics of turbulence in tokamaks.Spectrometers are developed to separate out the Doppler-shifted target line(122.03 and 122.17 nm)from the background Lyman-alpha line(121.53 nm).Here,30 Core-LAB channels and 30 Edge-LAB channels are under development on the HL-2A tokamak.It has high radial spatial resolutions of about 2.7 mm and 3.3 mm for the core and edge channels,respectively.Taking the high light intensity of this Lyman-alpha line into account,temporal resolution of 200 k Hz can be ensured by broad bandwidth amplifiers.This high spatio-temporal resolution makes LAB a potential keen tool to experimentally investigate tokamak plasma physics.
基金supported by the National Key Research and Development Program of China (Nos. 2019YFE0302002, 2017YFE0301203 and 2017YFE0301202)National Natural Science Foundation of China (No. 12175055)。
文摘A vertical edge Thomson scattering(ETS) diagnostic system on HL-2M tokamak has been designed.The ETS system collects the scattered light from Nd:YAG laser(1064 nm,2 J,30 Hz,15 ns).The laser beam propagates vertically through the plasma region and the polarization is parallel to the toroidal magnetic field.A special designed Galileo-type telescope with long Rayleigh length is applied to focus the laser size and ensure collimation.A group of doubleGaussian collection lenses image the 600 mm vertical scattered region onto rectangular fiber arrays with a spatial resolution of 10 mm.The 2.20 mm × 2.86 mm fiber optic bundle consists of 130 low hydroxyl(OH) 200/220 μm(core/cladding) diameter fibers with numerical aperture NA=0.22,carrying the light to remotely located multi-channel polychromators.Effect of oblique incidence on narrow band filter has been analyzed.The designed electron temperatures range from 5 to 1000 eV and electron densities from 5 × 10^(18) to 1×10^(20) m^(-3).
基金supported by National Natural Science Foundation of China(No.11535013)the National Key Research and Development Program of China(Nos.2017YFA0402500,2018YFE0302100)the Users with Excellence Project of Hefei Science Center CAS(No.2018HSC-UE010)
文摘Ion temperature, as one of the most critical plasma parameters, can be diagnosed by charge exchange recombination spectroscopy (CXRS). Iterative least-squares fitting is conventionally used to analyze CXRS spectra to identify the active charge exchange component, which is the result of local interaction between impurity ions with a neutral beam. Due to the limit of the time consumption of the conventional approach (~100 ms per frame), the Experimental Advanced Superconducting Tokamak CXRS data is now analyzed in-between shots. To explore the feasibility of real-time measurement, neural networks are introduced to perform fast estimation of ion temperature. Based on the same four-layer neural network architecture, two neural networks are trained for two central chords according to the ion temperature data acquired from the conventional method. Using the TensorFlow framework, the training procedures are performed by an error back-propagation algorithm with the regularization via the weight decay method. Good agreement in the deduced ion temperature is shown for the neural networks and the conventional approach, while the data processing time is reduced by 3 orders of magnitude (~0.1 ms per frame) by using the neural networks.
基金supported by the National Key R&D Program of China(Nos.2017YFE0301201 and 2018YFE0303102)National Natural Science Foundation of China(Nos.11875124 and 12075241).
文摘In this article,we present the optical design of a novel diagnostic on the HL-2A tokamak,i.e.the 20-channel edge Lyman-alpha beam emission spectroscopy,which is a promising solution for edge density turbulence research on tokamaks,as it offers the possibility of density fluctuation measurement with a 3.3 mm spatial resolution while maintains a high temporal resolution of 1μs.The optical path,including the reflective collection optics,the high-dispersion spectrometer,and the linear detector array,is carefully optimized to obtain a good image quality and a high throughput.The maximum root mean square radius of the collection optics is 64μm.The detected photon flux is estimated to be about 1011 photons/s/channel.
文摘【目的】丝氨酸/苏氨酸蛋白激酶K(Serine/Threonine protein kinases K)是分枝杆菌类似真核样的蛋白激酶,预测在分枝杆菌的生长和新陈代谢等生理过程中起着重要的作用,解析PknK的生物功能及作用机制,将为结核病的防治提供一定的理论基础。【方法】通过基因敲除等遗传方法获得结核分枝杆菌疫苗株BCG的pknK敲除菌株△pknK、回补菌株pMV361-pknK/△pknK和过表达菌株pMV261-pknK/BCG;对获得的菌株进行生长曲线测定和抗药性分析;通过pulldown-MS方法及生物信息学方法鉴定了PknK相互作用蛋白。【结果】监测各种分枝杆菌△pknK、pMV361-pknK/△pknK和pMV261-pknK/BCG生长,确定PknK负调控BCG生长;抗药性分析显示PknK降低BCG的耐药性;pulldown-MS方法显示PknK与丝氨酸/苏氨酸蛋白激酶PknA和双组分系统中的反应调节因子MtrA、TrcR、MoxR等蛋白相互作用。【结论】研究发现PknK调控分枝杆菌的生长和耐药性,我们的研究为深入研究PknK在结核分枝杆菌中的功能奠定了基础。
基金National Natural Science Foundation of China(11774288,11974279)Natural Science Foundation of Shaanxi Province(2019JC-25,2019-JM131,2019JM-236)。
文摘Understanding and controlling defect in two-dimensional materials is important for both linear and nonlinear optoelectronic devices,especially in terms of tuning nonlinear optical absorption.Taking advantage of an atomic defect formed easily by smaller size,molybdenum disulfide nanosheet is prepared successfully with a different size by gradient centrifugation.Interestingly,size-dependent sulfur vacancies are observed by high-resolution X-ray photoelectron spectroscopy,atomic force microscopy,and transmission electron microscopy.The defect effect on nonlinear absorption is investigated by Z-scan measurement at the wavelength of 800 nm.The results suggest the transition from saturable absorption to reverse saturable absorption can be observed in both dispersions and films.First principle calculations suggest that sulfur vacancies act as the trap state to capture the excited electrons.Moreover,an energy-level model with the trap state is put forward to explain the role of the sulfur vacancy defect in nonlinear optical absorption.The results suggest that saturable absorption and reverse saturable absorption originate from the competition between the excited,defect state and ground state absorption.Our finding provides a way to tune the nonlinear optical performance of optoelectronic devices by defect engineering.
基金supported by the National Natural Science Foundation of China(12074311,11774288,11974279)the Natural Science Foundation of Shaanxi Province(2019JC-25)。
文摘Heterostructures based on new advanced materials offer a cornerstone for future optoelectronic devices with improved photoelectric performance.Band alignment is crucial for understanding the mechanism of charge carrier transportation and interface dynamics in heterostructures.Herein,we grew SnS_(2)/Bi_(2)X_(3)(X=Se,Te)van der Waals heterostructures by combining physical vapor deposition with chemical vapor deposition.The band alignment,measured by high-resolution X-ray photoelectron spectroscopy,suggested the successful design of type-Ⅰ SnS_(2)/Bi_(2)Te_(3) and type-Ⅱ SnS_(2)/Bi_(2)Te_(3) heterostructures.The SnS_(2)/Bi_(2)X_(3) heterostructure greatly improved the photoelectric response of a photoelectrochemical-type photodetector.The photocurrent densities in the type-Ⅰ SnS_(2)/Bi_(2)Te_(3) and type-Ⅱ SnS_(2)/Bi_(2)Te_(3) heterostructure-based devices were more than one order of magnitude higher than those of SnS_(2),Bi_(2)Te_(3),and Bi_(2)Te_(3).The improved photoelectric properties of the SnS_(2)/Bi_(2)X_(3) heterostructures can be explained as follows:(i)the photoexcited electrons and holes are effectively separated in the heterostructures;(ii)the charge-transfer efficiency and carrier density at the interface between the SnS_(2)/Bi_(2)X_(3) heterostructures and the electrolyte are greatly improved;(iii)the formed heterostructures expand the light absorption range.The photoelectric performance was further enhanced by efficient light trapping in the upright SnS_(2).The photoelectric response is higher in the type-Ⅰ SnS_(2)/Bi_(2)Te_(3) heterostructure than in the type-Ⅱ SnS_(2)/Bi_(2)Te_(3) heterostructure due to more efficient charge transportation at the type-Ⅰ SnS_(2)/Bi_(2)Te_(3) heterostructure/electrolyte interface.These results suggest that suitable type-Ⅰ and type-Ⅱ heterostructures can be developed for high-performance photodetectors and other optoelectronic devices.